中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2014年
25期
7-9
,共3页
任兆强%王相海%林存智%朱新红%刘菲
任兆彊%王相海%林存智%硃新紅%劉菲
임조강%왕상해%림존지%주신홍%류비
胸腔积液%结核,肺%肿瘤%趋化因子
胸腔積液%結覈,肺%腫瘤%趨化因子
흉강적액%결핵,폐%종류%추화인자
Pleural effusion%Tuberculosis,pulmonary%Neoplasm%Chemokine
目的 探讨趋化因子γ-干扰素诱导蛋白-10(IP-10)、巨噬细胞炎性蛋白-1 α(MIP-1 α)、单核细胞趋化因子-l(MCP-1)对结核性和恶性胸腔积液的鉴别诊断价值.方法 采用双抗体夹心酶联免疫吸附测定法(ELISA)分别检测43例结核性胸腔积液(结核性胸腔积液组)和45例恶性胸腔积液(恶性胸腔积液组)中IP-10、MIP-1 α、MCP-1的表达.应用ROC曲线分析两组胸腔积液中IP-10、MIP-1 α、MCP-1的表达差异及意义.结果结核性胸腔积液组IP-10、MIP-1α、MCP-1水平显著高于恶性胸腔积液组,差异有统计学意义(t=4.931,3.106,2.385;P=0.000,0.004,0.041).ROC曲线分析结果 显示胸腔积液中IP-10、MIP-l α、MCP-1的诊断临界值为1 589.73,213.50,1452.63 ng/L;诊断敏感度分别为68.8％,81.3％,87.5％;特异度分别为87.5％,68.8％,56.3％.结论 胸腔积液中IP-10、MIP-1 α、MCP-1表达对结核性和肿瘤性胸腔积液诊断与鉴别诊断具有重要参考价值.
目的 探討趨化因子γ-榦擾素誘導蛋白-10(IP-10)、巨噬細胞炎性蛋白-1 α(MIP-1 α)、單覈細胞趨化因子-l(MCP-1)對結覈性和噁性胸腔積液的鑒彆診斷價值.方法 採用雙抗體夾心酶聯免疫吸附測定法(ELISA)分彆檢測43例結覈性胸腔積液(結覈性胸腔積液組)和45例噁性胸腔積液(噁性胸腔積液組)中IP-10、MIP-1 α、MCP-1的錶達.應用ROC麯線分析兩組胸腔積液中IP-10、MIP-1 α、MCP-1的錶達差異及意義.結果結覈性胸腔積液組IP-10、MIP-1α、MCP-1水平顯著高于噁性胸腔積液組,差異有統計學意義(t=4.931,3.106,2.385;P=0.000,0.004,0.041).ROC麯線分析結果 顯示胸腔積液中IP-10、MIP-l α、MCP-1的診斷臨界值為1 589.73,213.50,1452.63 ng/L;診斷敏感度分彆為68.8％,81.3％,87.5％;特異度分彆為87.5％,68.8％,56.3％.結論 胸腔積液中IP-10、MIP-1 α、MCP-1錶達對結覈性和腫瘤性胸腔積液診斷與鑒彆診斷具有重要參攷價值.
목적 탐토추화인자γ-간우소유도단백-10(IP-10)、거서세포염성단백-1 α(MIP-1 α)、단핵세포추화인자-l(MCP-1)대결핵성화악성흉강적액적감별진단개치.방법 채용쌍항체협심매련면역흡부측정법(ELISA)분별검측43례결핵성흉강적액(결핵성흉강적액조)화45례악성흉강적액(악성흉강적액조)중IP-10、MIP-1 α、MCP-1적표체.응용ROC곡선분석량조흉강적액중IP-10、MIP-1 α、MCP-1적표체차이급의의.결과결핵성흉강적액조IP-10、MIP-1α、MCP-1수평현저고우악성흉강적액조,차이유통계학의의(t=4.931,3.106,2.385;P=0.000,0.004,0.041).ROC곡선분석결과 현시흉강적액중IP-10、MIP-l α、MCP-1적진단림계치위1 589.73,213.50,1452.63 ng/L;진단민감도분별위68.8％,81.3％,87.5％;특이도분별위87.5％,68.8％,56.3％.결론 흉강적액중IP-10、MIP-1 α、MCP-1표체대결핵성화종류성흉강적액진단여감별진단구유중요삼고개치.
Objective To study the differential diagnostic value of interferon-γ inducible protein 10 (IP-10),macrophage inflammatory protein-1 α (MIP-1 α) and monocyte chemoattractant-1 (MCP-1) level in the tuberculous,malignant pleural effusion.Methods Enzyme-linked immunosorbent assay was used to detect the level of IP-10,MIP-1 α and MCP-1 in tuberculous pleural fluid (tuberculous pleural fluid group,43 cases) and malignant pleural fluid (malignant pleural fluid group,45 cases).The level of IP-10,MIP-1 α and MCP-1 and the significance were analyzed by ROC curve.Results The level of IP-10,MIP-1 α and MCP-1 were significantly higher in tuberculous pleural fluid group than those in malignant pleural fluid group,and there were significant differences(t =4.931,3.106,2.385 ; P =0.000,0.004,0.041).ROC curve analysis showed that the critical value of IP-10,MIP-1 α and MCP-1 in diagnosis of pleural effusion was respectively 1 589.73,213.50,1 452.63 ng/L.The sensitivity and specificity of IP-10,MIP-1 α and MCP-1 in pleural fluid were 68.8％,81.3％,87.5％ and 87.5％,68.8％,56.3％,respectively.Conclusion The level of IP-10,MIP-1 α and MCP-1 in tuberculous and malignant pleural fluid are significant for the early diagnosis and differential diagnosis.
