CAJ | 학술논문

目的探讨大肠癌miR-195表达变化与临床病理学特征的关系.方法采用实时荧光定量PCR技术检测miR-195在50例大肠癌患者癌组织及对应癌旁正常组织(距离癌组织＞5 cm)中的表达水平,分析其表达情况与临床病理因素的相关性.结果 50例大肠癌患者中共检出miR-195 CpG岛甲基子表型阳性者29例,阳性率为58％(29/50).50例大肠癌患者中有34例出现miR-195在大肠癌组织中的表达较癌旁正常组织下调,即T/N＜2.miR-195在大肠癌组织中及癌旁正常组织中的表达水平分别为0.837±1.145和2.236±2.468,差异有统计学意义(P＜0.05).miR-195在大肠癌组织中的低表达与患者的年龄、性别、大肠癌部位、浸润深度无关(P＞0.05),与患者淋巴结转移、TNM分期有关(P＜0.05),在发生淋巴结转移或TNM分期晚的患者中表达明显下调.结论 miR-195在大肠癌组织中的表达呈普遍低表达的趋势,miR-195可能在大肠癌中扮演抑癌基因的角色.miR-195启动子区高甲基化可能是导致miR-195在大肠癌组织中表达下调及参与大肠癌发生发展的原因之一.
목적 탐토대장암miR-195표체변화여림상병이학특정적관계.방법 채용실시형광정량PCR기술검측miR-195재50례대장암환자암조직급대응암방정상조직(거리암조직＞5 cm)중적표체수평,분석기표체정황여림상병리인소적상관성.결과 50례대장암환자중공검출miR-195 CpG도갑기자표형양성자29례,양성솔위58％(29/50).50례대장암환자중유34례출현miR-195재대장암조직중적표체교암방정상조직하조,즉T/N＜2.miR-195재대장암조직중급암방정상조직중적표체수평분별위0.837±1.145화2.236±2.468,차이유통계학의의(P＜0.05).miR-195재대장암조직중적저표체여환자적년령、성별、대장암부위、침윤심도무관(P＞0.05),여환자림파결전이、TNM분기유관(P＜0.05),재발생림파결전이혹TNM분기만적환자중표체명현하조.결론 miR-195재대장암조직중적표체정보편저표체적추세,miR-195가능재대장암중분연억암기인적각색.miR-195계동자구고갑기화가능시도치miR-195재대장암조직중표체하조급삼여대장암발생발전적원인지일.
Objective To explore the relationship between miR-195 expression and clinical pathological characteristics of colorectal cancer.Methods The expression of miR-195 in 50 cases of colorectal cancer tissue and adjacent non-tumor mucosa (5 cm away from the cancer tissue) was detected by real time PCR.The relationship between miR-195 expression and clinical pathological characteristics of colorectal cancer was analyzed.Results miR-195 CpG island methylate phenotype was positive in 58％ (29/50) of patients with colorectal cancer.The expression level of miR-195 in 34 patients colorectal cancer tissue was lower than that in adjacent non-tumor mucosa,that was T/N ＜ 2.The expression level of miR-195 in carcinoma tissue was 0.837 ± 1.145,comparing with adjacent non-tumor mucosa (2.236 ± 2.468),showing significant difference (P ＜ 0.05).Down-regulated expression of miR-195 in carcinoma tissue was independent of age,gender,site(P ＞ 0.05),and related to lymphnode metastasis and TNM stage(P ＜ 0.05).Patients with lymph node metastasis and advanced TNM stage showed significantly reduced expression of miR-195.Conclusions The tendency of common low expression level of miR-195 in human colorectal cancer.MiR-195 maybe play as suppressor gene in human colon carcinoma.Hypermethylation of miR-195 promoter may be one of reasons leading to down-regulation of miR-195 in colorectal cancer tissues and participated in the occurrence and development of human colon carcinoma.