CAJ | 학술논문

目的研究大连地区人乳头瘤病毒(HPV)感染率、基因分型和年龄分布特征,确定本地区的优势型别及高风险人群,同时验证HPV基因检测方法与细胞学检测方法的差异.方法采用实时荧光定量PCR和基因芯片杂交分型技术对4177例门诊妇女进行HPV基因分型检测.分析HPV基因型感染分布特点,计算各年龄段妇女高危型HPV的感染率.对HPV病毒阳性患者进行细胞学检测,分析不同感染类型患者的细胞学阳性率差异.结果大连地区妇女HPV感染率为17.09％(711/4160),高危型HPV(hr-HPV)感染率为13.73％(572/4160),低危型HPV感染率为3.36％ (140/4160),感染率最高基因型是HPV16型(13.50％),其次为58、56、33、35、和68型.不同年龄妇女hr-HPV阳性率比较差异有统计学意义(P＜0.05),＜30岁妇女阳性率最高.高危型混合感染组的细胞学阳性率显著高于单一型hr-HPV感染组和低危型感染组.结论大连地区女性HPV感染率为17.09％,其中hr-HPV感染率为13.73％,低危型HPV感染率为3.36％.人群感染HPV优势型别为HPV16.年龄＜30岁是hr-HPV感染的高峰期.高危型混合感染的细胞学阳性率高于其他感染类型.
목적 연구대련지구인유두류병독(HPV)감염솔、기인분형화년령분포특정,학정본지구적우세형별급고풍험인군,동시험증HPV기인검측방법여세포학검측방법적차이.방법 채용실시형광정량PCR화기인심편잡교분형기술대4177례문진부녀진행HPV기인분형검측.분석HPV기인형감염분포특점,계산각년령단부녀고위형HPV적감염솔.대HPV병독양성환자진행세포학검측,분석불동감염류형환자적세포학양성솔차이.결과 대련지구부녀HPV감염솔위17.09％(711/4160),고위형HPV(hr-HPV)감염솔위13.73％(572/4160),저위형HPV감염솔위3.36％ (140/4160),감염솔최고기인형시HPV16형(13.50％),기차위58、56、33、35、화68형.불동년령부녀hr-HPV양성솔비교차이유통계학의의(P＜0.05),＜30세부녀양성솔최고.고위형혼합감염조적세포학양성솔현저고우단일형hr-HPV감염조화저위형감염조.결론 대련지구녀성HPV감염솔위17.09％,기중hr-HPV감염솔위13.73％,저위형HPV감염솔위3.36％.인군감염HPV우세형별위HPV16.년령＜30세시hr-HPV감염적고봉기.고위형혼합감염적세포학양성솔고우기타감염류형.
Objective Research Dalian region of human papillomavirus infection rate,genotype and age distribution.Determine the area advantage type and high risk population,at the same time to verify that the HPV gene detection method and method of cytological examination of differences.Methods The real-time fluorescence quantitative PCR and gene chip hybridization typing technique in 4177 cases of Clinic Women for HPV genotyping assay.Analysis of genotype HPV infection distribution characteristics,calculation of the age in women at high risk of HPV infection rate.On HPV virus positive patients for cytology test,analysis of different types of infection patient cytology positive rate difference.Results Dalian region women's HPV infection rate was 17.09％ (711/4160),hr-HPV infection rate was 13.73％ (572/4160),low risk type HPV infection rate was 3.36％ (140/4160),the highest infection rate in genotype HPV16 (13.50％),followed by 58,56,33,35,and 68 type.The positive rate of hr-HPV between women of different age groups showed significant difference (P＜ 0.05),the positive rate was the highest in ＜ 30 years old.High risk group of mixed infection of cytology positive rate was higher than that of single hr-HPV infection group and the low risk of infection group.Conclusions Local people infected with HPV advantage of type HPV16.The positive rate of hr-HPV was associated with age,＜ 30 years is the peak of infection of high risk hr-HPV.The mixed infection of cytologically positive rate than other types of infection.