CAJ | 학술논문

目的应用高分辨魔角旋转磁共振(HR-MAS NMR)波谱分析巴马小型猪阻塞性慢性胰腺炎(CP)早期的代谢变化特征.方法 39头巴马小型猪按随机数字表法分为对照组和CP组.采用主胰管近端不全结扎法制备CP模型,对照组仅开腹翻动胰腺及肠道后关腹.造模后第4、8、12周分批处死动物,获取胰腺组织.根据胰腺纤维化程度,将CP分为轻、中、重度.应用HR-MAS NMR波谱分析轻度CP组和对照组胰腺组织的代谢特征.结果制模成功的24头巴马小型猪的胰腺组织经病理检查为轻度10头,中度5头,重度7头.经HR-MAS NMR波谱分析,轻度CP组胰腺组织的代谢产物中胆碱、甘氨酸、甜菜碱、乳酸、脂肪酸、磷酸胆碱/甘油磷酸胆碱峰值与对照组有差异,其中胆碱波峰显著增高,差异有统计学意义(5.02 ±1.96比1.56 ±0.79,P＜0.01),而其他5种代谢产物峰的差异均无统计学意义.结论与对照组比较,巴马小型猪轻度CP胰腺组织的胆碱波峰强度显著增高,这可能是区分正常和早期CP的标志.
목적 응용고분변마각선전자공진(HR-MAS NMR)파보분석파마소형저조새성만성이선염(CP)조기적대사변화특정.방법 39두파마소형저안수궤수자표법분위대조조화CP조.채용주이관근단불전결찰법제비CP모형,대조조부개복번동이선급장도후관복.조모후제4、8、12주분비처사동물,획취이선조직.근거이선섬유화정도,장CP분위경、중、중도.응용HR-MAS NMR파보분석경도CP조화대조조이선조직적대사특정.결과 제모성공적24두파마소형저적이선조직경병리검사위경도10두,중도5두,중도7두.경HR-MAS NMR파보분석,경도CP조이선조직적대사산물중담감、감안산、첨채감、유산、지방산、린산담감/감유린산담감봉치여대조조유차이,기중담감파봉현저증고,차이유통계학의의(5.02 ±1.96비1.56 ±0.79,P＜0.01),이기타5충대사산물봉적차이균무통계학의의.결론 여대조조비교,파마소형저경도CP이선조직적담감파봉강도현저증고,저가능시구분정상화조기CP적표지.
Objective To investigate the metabolic characteristics of early stage of obstructive chronic pancreatitis (CP) in swine by high-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy.Methods A total of 39 Bama miniature swine were randomly divided into CP group and control group.The pancreatic main duct (PMD) of the swine in the experimental group underwent partial proximal ligation procedure.The swine in the control group underwent opening the abdomen and rubbing the pancreas and intestine.At the 4th,8th and 12th week after the operation,the swine were sacrificed to harvest the pancreas tissue for pathologically examination,which were classified as mild,moderate and severe according to the degree of fibrosis.The metabolic profiles from the pancreas of normal swine and mild CP swine were examined by using HR-MAS NMR spectroscopy.Results CP model was successfully established in 24 Bama miniature swine,and pathologically examination showed 10 was mild,5 was moderate and 7 was severe.Among the metabolites in mild CP,HR-MAS NMR analysis suggested that the peak of choline,glycine,betaine,lactate,fatty acid,phosphorylcholine/choline glycerophosphate was different with those in normal pancreas,and only the difference of choline was statistically significant (5.02 ± 1.96 vs 1.56 ± 0.79,P ＜ 0.O1),while the difference of other 5 metabolites was not statistically significant.Conclusions Compared with control group,mild CP in Bama miniature swine shows significantly increased choline,and this may be a marker for discrimination of normal and early CP.