CAJ | 학술논문

目的检测5株人胰腺癌细胞株中TM4SF1 mRNA的表达,探讨TM4SF1基因对癌细胞增殖、迁移及侵袭能力的影响.方法采用实时PCR方法检测人胰腺癌细胞株MPanc96、MiaPaCa-2、HPAC、PANC1、AsPC-1的TM4SF1 mRNA表达,并与人正常胰腺导管上皮细胞(HPDE)的TM4SF1mRNA表达进行比较.应用RNA干扰方法将靶向TM4SF1的siRNA及阴性对照siRNA瞬时转染MPanc96、MiaPaCa-2细胞.采用MTS法检测转染细胞的增殖能力,Transwell小室法检测细胞的迁移及侵袭能力.结果胰腺癌细胞株MPanc96、MiaPaCa-2、PANC1、AsPC-1、HPAC的TM4SF1 mRNA相对表达量分别为1.205±0.073、1.096±0.260、1.382±0.075、1.374±0.363、0.744±0.096,均显著高于HPDE的0.020±0.003(F =22.26,P＜0.01).与转染阴性对照siRNA的细胞比较,TM4SF1基因表达沉默的MPanc96、MiaPaCa-2细胞的增殖无显著变化,但细胞的迁移力分别降低(62.5±7.6)％、(72.8±4.0)％,侵袭能力分别下降(69.5±5.7)％、(78.6±6.3)％.结论 TM4SF1在人胰腺癌细胞中高表达,并增强胰腺癌细胞的迁移和侵袭能力.
목적 검측5주인이선암세포주중TM4SF1 mRNA적표체,탐토TM4SF1기인대암세포증식、천이급침습능력적영향.방법 채용실시PCR방법검측인이선암세포주MPanc96、MiaPaCa-2、HPAC、PANC1、AsPC-1적TM4SF1 mRNA표체,병여인정상이선도관상피세포(HPDE)적TM4SF1mRNA표체진행비교.응용RNA간우방법장파향TM4SF1적siRNA급음성대조siRNA순시전염MPanc96、MiaPaCa-2세포.채용MTS법검측전염세포적증식능력,Transwell소실법검측세포적천이급침습능력.결과 이선암세포주MPanc96、MiaPaCa-2、PANC1、AsPC-1、HPAC적TM4SF1 mRNA상대표체량분별위1.205±0.073、1.096±0.260、1.382±0.075、1.374±0.363、0.744±0.096,균현저고우HPDE적0.020±0.003(F =22.26,P＜0.01).여전염음성대조siRNA적세포비교,TM4SF1기인표체침묵적MPanc96、MiaPaCa-2세포적증식무현저변화,단세포적천이력분별강저(62.5±7.6)％、(72.8±4.0)％,침습능력분별하강(69.5±5.7)％、(78.6±6.3)％.결론 TM4SF1재인이선암세포중고표체,병증강이선암세포적천이화침습능력.
Objective To determine the expression of TM4SF1 mRNA in 5 human pancreatic cancer cell lines,and investigate its effect on the proliferation,migration and invasion of pancreatic cancer cells.Methods The expression of TM4SF1 mRNA in MPanc96,MiaPaCa-2,PANC1,AsPC-1,HPAC cells was determined by qRT-PCR,and the results were compared with that of human pancreatic ductal epithelial (HPDE) cells.RNA interference method was used to transiently transfect siRNA targeting at TM4SF1 and negative control siRNA into MPanc96,MiaPaCa-2 cells.The proliferation of cells were measured by MTS method,and migration and invasion of cells were determined by Transwell.Results The expression levels of TM4SF1 mRNA in pancreatic cancer cell lines MPanc96,MiaPaCa-2,PANC1,AsPC-1 and HPAC were 1.205 ± 0.073,1.096 ± 0.260,1.382 ± 0.075,1.374 ± 0.363 and 0.744 ± 0.096,which were significantly highly than that in HPDE (0.020 ± 0.003,P ＜ 0.01).Compared with cells transfected with negative control siRNA,the proliferation of MPanc96 and MiaPaCa-2 cells transfected with siRNA targeting at TM4SF1 was not significantly changed,but the migration abilitiy was decreased by (62.5 ± 7.6) ％ and (72.8 ± 4.0) ％,and invasion abilitiy was decreased by (69.5 ± 5.7) ％ and (78.6 ± 6.3) ％.Conclusions TM4SF1 is highly expressed in pancreatic cancer cells and appears to promote the migration and invasion abilities of the cancer cells.