CAJ | 학술논문

目的探讨鸟嘌呤核苷酸解离抑制因子2 (Rho-GDI2)基因在胰腺癌组织中的表达及其临床意义.方法运用RT-PCR技术和免疫组化技术检测60例胰腺癌组织及配对的癌旁组织中Rho-GDI2 mRNA和蛋白的表达,分析胰腺癌Rho-GDI2的表达与临床病理参数间的关系.结果胰腺癌组织及相应癌旁组织中Rho-GDI2 mRNA表达量分别为0.661±0.021和0.199±0.023;Rho-GDI2蛋白阳性表达率分别为73.3％ (44/60)和41.7％(25/60),癌组织的表达均显著高于癌旁组织,差异有统计学意义(P值均＜0.01).胰腺癌组织Rho-GDI2蛋白表达与患者性别、年龄及肿瘤部位无相关性,而与肿瘤大小、分化程度、病理分期、淋巴结转移、血管侵犯等相关(P值均＜0.05).结论 Rho-GDI2在胰腺癌组织中过表达,且与胰腺癌的恶性生物学行为密切相关.
목적 탐토조표령핵감산해리억제인자2 (Rho-GDI2)기인재이선암조직중적표체급기림상의의.방법 운용RT-PCR기술화면역조화기술검측60례이선암조직급배대적암방조직중Rho-GDI2 mRNA화단백적표체,분석이선암Rho-GDI2적표체여림상병리삼수간적관계.결과 이선암조직급상응암방조직중Rho-GDI2 mRNA표체량분별위0.661±0.021화0.199±0.023;Rho-GDI2단백양성표체솔분별위73.3％ (44/60)화41.7％(25/60),암조직적표체균현저고우암방조직,차이유통계학의의(P치균＜0.01).이선암조직Rho-GDI2단백표체여환자성별、년령급종류부위무상관성,이여종류대소、분화정도、병리분기、림파결전이、혈관침범등상관(P치균＜0.05).결론 Rho-GDI2재이선암조직중과표체,차여이선암적악성생물학행위밀절상관.
Objective To investigate the expression and clinical significance of Rho GDP dissociation inhibitor 2 (Rho-GDI2) in pancreatic cancer.Methods Immunohistochemistry and RT-PCR were used to evaluate the expression of Rho-GDI2 in 60 pairs of pancreatic cancer tissues and adjacent pancreatic tissues,and its correlation with clinicopathological parameter of pancreatic cancer was also analyzed.Results The expressions of Rho-GDI2 mRNA were 0.661 ± 0.021 and 0.199 ± 0.023 in pancreatic cancer tissues and adjacent pancreatic tissues,and the positive rate of Rho-GDI2 protein expression were 73.3％ (44/60) and 41.7％ (25/60),the positive rate of Rho-GDI2 expression in pancreatic cancer tissues was higher than that of adjacent normal tissues,and the difference between the two groups was statistically significant (P ＜ 0.01).The expression of Rho-GDI2 was strongly correlated with tumor size,differentiation,staging,lymph node metastasis,vascular invasion (P＜0.05),but it was not associated with gender,age,tumor location (P ＞0.05).Conclusions Rho-GDI2 expression is up-regulated in pancreatic cancer,and is strongly correlated with the malignant biological behavior of pancreatic cancer.