中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2014年
4期
235-237
,共3页
吴汉青%王博%薛寅凯%郑海%陈立波%吴河水
吳漢青%王博%薛寅凱%鄭海%陳立波%吳河水
오한청%왕박%설인개%정해%진립파%오하수
胰腺肿瘤%Toll样受体9%CpG ODN2216%吉西他滨
胰腺腫瘤%Toll樣受體9%CpG ODN2216%吉西他濱
이선종류%Toll양수체9%CpG ODN2216%길서타빈
Pancreatic neoplasms%Toll like receptor 9%CpG ODN2216%Gemcitabine
目的 观察用Toll样受体9(TLR9)激动剂CpG ODN2216处理后胰腺癌PANC1细胞对吉西他滨化疗敏感性的影响.方法 采用免疫荧光化学法及蛋白质印迹法检测胰腺癌PANC1细胞TLR9蛋白的表达,应用四甲基偶氮唑蓝(MTT)比色法检测CpG ODN2216处理后PANC1细胞对不同浓度吉西他滨敏感性的变化.结果 胰腺癌PANC1细胞高表达TLR9蛋白.CpG ODN2216±吉西他滨组的PANC1细胞对吉西他滨的半数抑制剂量(IC50)为(0.28 ±0.13) mg/L,吉西他滨组PANC1细胞的IC5o为(1.23±0.14) mg/L,两组差异有统计学意义(P＜0.01).0.01、0.1、1、10 mg/L吉西他滨干预经CpGODN2216处理的PANC1细胞的生长抑制率分别为(34.1±1.3)％、(43.5±2.7)％、(76.3±2.5)％、(95.3±2.2)％;干预未经CpG ODN2216处理的PANC1细胞的生长抑制率分别为(14.5±0.9)％、(23.5±1.1)％、(44.8±1.4)％、(63.6±1.8)％,两组差异均有统计学意义(P值均＜0.01).结论 TLR9激动剂CpG ODN2216能增加人胰腺癌细胞PANC1对吉西他滨的敏感性.
目的 觀察用Toll樣受體9(TLR9)激動劑CpG ODN2216處理後胰腺癌PANC1細胞對吉西他濱化療敏感性的影響.方法 採用免疫熒光化學法及蛋白質印跡法檢測胰腺癌PANC1細胞TLR9蛋白的錶達,應用四甲基偶氮唑藍(MTT)比色法檢測CpG ODN2216處理後PANC1細胞對不同濃度吉西他濱敏感性的變化.結果 胰腺癌PANC1細胞高錶達TLR9蛋白.CpG ODN2216±吉西他濱組的PANC1細胞對吉西他濱的半數抑製劑量(IC50)為(0.28 ±0.13) mg/L,吉西他濱組PANC1細胞的IC5o為(1.23±0.14) mg/L,兩組差異有統計學意義(P＜0.01).0.01、0.1、1、10 mg/L吉西他濱榦預經CpGODN2216處理的PANC1細胞的生長抑製率分彆為(34.1±1.3)％、(43.5±2.7)％、(76.3±2.5)％、(95.3±2.2)％;榦預未經CpG ODN2216處理的PANC1細胞的生長抑製率分彆為(14.5±0.9)％、(23.5±1.1)％、(44.8±1.4)％、(63.6±1.8)％,兩組差異均有統計學意義(P值均＜0.01).結論 TLR9激動劑CpG ODN2216能增加人胰腺癌細胞PANC1對吉西他濱的敏感性.
목적 관찰용Toll양수체9(TLR9)격동제CpG ODN2216처리후이선암PANC1세포대길서타빈화료민감성적영향.방법 채용면역형광화학법급단백질인적법검측이선암PANC1세포TLR9단백적표체,응용사갑기우담서람(MTT)비색법검측CpG ODN2216처리후PANC1세포대불동농도길서타빈민감성적변화.결과 이선암PANC1세포고표체TLR9단백.CpG ODN2216±길서타빈조적PANC1세포대길서타빈적반수억제제량(IC50)위(0.28 ±0.13) mg/L,길서타빈조PANC1세포적IC5o위(1.23±0.14) mg/L,량조차이유통계학의의(P＜0.01).0.01、0.1、1、10 mg/L길서타빈간예경CpGODN2216처리적PANC1세포적생장억제솔분별위(34.1±1.3)％、(43.5±2.7)％、(76.3±2.5)％、(95.3±2.2)％;간예미경CpG ODN2216처리적PANC1세포적생장억제솔분별위(14.5±0.9)％、(23.5±1.1)％、(44.8±1.4)％、(63.6±1.8)％,량조차이균유통계학의의(P치균＜0.01).결론 TLR9격동제CpG ODN2216능증가인이선암세포PANC1대길서타빈적민감성.
Objective To investigate the effects of toll-like receptor 9 (TLR9) agonist CpG ODN2216 on the sensitivity of pancreatic cancer cell line PANC1's to gemcitabine.Methods The immunofluorescence staining method and Western blot method were used to examine the expression of TLR9 protein in PANC1 cells.The changes of sensitivity to gemcitabine after CpG ODN2216 treatment were examined by MTT assay.Results The TLR9 protein was highly expressed in PANC1 cells and the median inhibition concentration of gemcitabine against PANC1 cells was reduced from (1.23 ± 0.14) mg/L to (0.28 ± 0.13) mg/L after CpG ODN2216 treatment,and the difference between the two groups was statistically significant (P ＜0.01).After 0.01,0.10,1.00,10.00 mg/L gemcitabine treatment with CpG 0DN2216,the inhibition rates of PANC1 were (34.4 ±1.3)％,(43.5 ± 2.7)％,(76.3 ± 2.5)％,(95.3 ± 2.2)％ ; and without CpG ODN2216,the inhibition rates of PANC1 were (14.5 ± 0.9) ％,(23.5 ± 1.1) ％,(44.8 ± 1.4) ％,(63.6 ± 1.8) ％,and the difference between the two groups was statistically significant (P ＜ 0.01).Conclusions The sensitivity of PANC1 cells to gemcitabine can be enhanced by CpG ODN2216.
