CAJ | 학술논문

目的观察参麦注射液对急性水肿性胰腺炎(AEP)大鼠的治疗作用,探讨其作用机制.方法采用腹腔内注射雨蛙素连续7次、间隔1h的方法建立大鼠AEP模型.制模后1h,参麦组给予静脉滴注参麦注射液(5 ml·kg-1 ·d-1)治疗,AEP组给予等容积生理盐水.建模后1h及1、3、5、7d眼内眦交替采血,采用酶联免疫吸附法检测血清淀粉酶、血小板活化因子(PAF)、血管内皮生长因子(VEGF)的含量.术后7、14 d分两批处死大鼠,取胰腺组织.采用实时RT-PCR和蛋白质印迹法检测胰腺组织NF-κB mRNA和蛋白的表达量,以正常胰腺组织为对照.采用免疫组化法检测胰腺组织CD31的表达,计算微血管密度(MVD).结果两组造模后血清淀粉酶活性及PAF含量均升高,3d后逐渐下降,参麦组的下降较AEP组显著.造模后5d参麦组、AEP组的淀粉酶活性分别为(4569±158)、(5056±198) U/L,PAF分别为(25.30±4.76)、(36.06±5.57) μg/L,两组的差异均具有统计学意义(P值均＜0.05).两组大鼠血清VEGF含量于造模后1d开始逐渐升高,3d后参麦组的升高较AEP组显著,5d时分别为(139.78±24.30)、(118.51±21.70) pg/ml,两组差异具有统计学意义(P＜0.05).术后7d参麦组胰腺组织NF-κB mRNA和蛋白相对表达量分别为0.834±0.031和0.49±0.24,MVD为6.41±1.14;AEP组分别为1.000±0.059、0.93 ±0.45、3.62±0.89,两组差异均具有统计学意义(P＜0.05或＜0.01).结论参麦注射液治疗急性胰腺炎具有促进血管生成、改善微循环、减轻炎症递质瀑布反应的作用.
목적 관찰삼맥주사액대급성수종성이선염(AEP)대서적치료작용,탐토기작용궤제.방법 채용복강내주사우와소련속7차、간격1h적방법건립대서AEP모형.제모후1h,삼맥조급여정맥적주삼맥주사액(5 ml·kg-1 ·d-1)치료,AEP조급여등용적생리염수.건모후1h급1、3、5、7d안내자교체채혈,채용매련면역흡부법검측혈청정분매、혈소판활화인자(PAF)、혈관내피생장인자(VEGF)적함량.술후7、14 d분량비처사대서,취이선조직.채용실시RT-PCR화단백질인적법검측이선조직NF-κB mRNA화단백적표체량,이정상이선조직위대조.채용면역조화법검측이선조직CD31적표체,계산미혈관밀도(MVD).결과 량조조모후혈청정분매활성급PAF함량균승고,3d후축점하강,삼맥조적하강교AEP조현저.조모후5d삼맥조、AEP조적정분매활성분별위(4569±158)、(5056±198) U/L,PAF분별위(25.30±4.76)、(36.06±5.57) μg/L,량조적차이균구유통계학의의(P치균＜0.05).량조대서혈청VEGF함량우조모후1d개시축점승고,3d후삼맥조적승고교AEP조현저,5d시분별위(139.78±24.30)、(118.51±21.70) pg/ml,량조차이구유통계학의의(P＜0.05).술후7d삼맥조이선조직NF-κB mRNA화단백상대표체량분별위0.834±0.031화0.49±0.24,MVD위6.41±1.14;AEP조분별위1.000±0.059、0.93 ±0.45、3.62±0.89,량조차이균구유통계학의의(P＜0.05혹＜0.01).결론 삼맥주사액치료급성이선염구유촉진혈관생성、개선미순배、감경염증체질폭포반응적작용.
Objective To observe the therapeutic effect of Shenmai injection on rats with acute edematous pancreatitis (AEP) and its mechanism.Methods The model of AEP in rats was induced by intraperitoneal injection of caerulein every one hour for 7 times.One hour after last injection,intravenous Shenmai injection (5 ml · kg-1 · d-1) was given to rats in Shenmai injection treatment (SI) group,while same amount of normal saline was given to rats in control (AEP) group.One hour and 1,3,5,7 d after AEP induction,the blood was taken,and the method of double antibody sandwich enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of amylase,platelet activating factor (PAF),vascular endothelial growth factor (VEGF).The rats were sacrificed at 7 and 14 d,and pancreatic tissue samples were harvested.RT-PCR and Western Blot were used to determine the expression of NF-κB mRNA and protein,and normal pancreatic tissue samples were used as control.The positive expression of CD31 in pancreatic tissue was determined by immunohistochemical method to calculate microvascular density (MVD).Results The serum levels of amylase and PAF in both groups were increased and gradually decreased 3 days later,while the decrement in SI group was greater than that of AEP group.At the 5th day after AEP induction,the serum levels of amylase in SI group and AEP group were (4569 ± 158),(5056 ± 198) U/L,and the serum levels of PAF were (25.30 ± 4.76),(36.06 ± 5.57)μg/L,and the difference between the two groups was statistically significant (P ＜ 0.05).The serum levels of VEGF were increased 1 d after AEP induction,and the increase in SI group was greater than that of AEP group 3 d later,and the serum levels of VEGF were (139.78 ±24.30),(118.51 ±21.70)pg/ml at 5th day,and the difference between the two groups was statistically significant (P ＜ 0.05).The expression levels of NF-κB mRNA and protein of pancreatic tissue in SI group were O.834 ±0.031 and 0.49±0.24,and MVD was 6.41 ± 1.14,while the corresponding values in AEP group were 1.000 ± 0.059,0.93 ± 0.45,3.62 ± 0.89,and the difference between the two groups was statistically significant (P ＜ 0.05 or P ＜ 0.01).Conclusions In the course of acute pancreatitis,Shenmai injection has the therapeutic effects of promoting new angiogenesis,improving the microcirculation,reducing the inflammatory cascade.