我国鼠疫耶尔森菌单核苷酸多态性特征研究
아국서역야이삼균단핵감산다태성특정연구
Single nucleotide polymorphisms characteristics of the Yersinia Pestis in China
  • 万方数据
    中国地方病学杂志 중국지방병학잡지
    CHINESE JOURNAL OF ENDEMIOLOGY
    2012年 5期 476-481 ,共6页

    王娜%申小娜%俞东征%夏连续%魏建春%蔡虹%徐冬蕾%陈晨%崔志刚%梁莹%徐大琴%雒涛%海荣

    왕나%신소나%유동정%하련속%위건춘%채홍%서동뢰%진신%최지강%량형%서대금%락도%해영

    耶尔森菌,鼠疫%多态性,单核苷酸%聚类分析 耶爾森菌,鼠疫%多態性,單覈苷痠%聚類分析
    야이삼균,서역%다태성,단핵감산%취류분석
    Yersinia pestis%Polymorphism,single nucleotide%Cluster analysis
    目的 了解我国不同鼠疫自然疫源地鼠疫耶尔森菌的单核苷酸多态性(SNP)特征.方法 利用Mummer程序对已知全基因组序列的9株鼠疫菌进行全基因组比对,选择其中的13个基因片段(19个SNP位点)在国内133株鼠疫菌中进行PCR扩增、测序,并对序列进行UPGMA聚类分析.结果 全基因组比对共筛查出3780处序列差异位点,通过对不同SNP位点组合的聚类分析发现我国鼠疫菌具有明显的地理区域性和生态型聚集性特征.结论 SNP作为一种比较稳定的遗传标记,可以较好地反映我国不同鼠疫自然疫源地耶尔森鼠疫菌的基因组特征.
    목적 료해아국불동서역자연역원지서역야이삼균적단핵감산다태성(SNP)특정.방법 이용Mummer정서대이지전기인조서렬적9주서역균진행전기인조비대,선택기중적13개기인편단(19개SNP위점)재국내133주서역균중진행PCR확증、측서,병대서렬진행UPGMA취류분석.결과 전기인조비대공사사출3780처서렬차이위점,통과대불동SNP위점조합적취류분석발현아국서역균구유명현적지리구역성화생태형취집성특정.결론 SNP작위일충비교은정적유전표기,가이교호지반영아국불동서역자연역원지야이삼서역균적기인조특정.
    Objective To study the single nucleotide polymorphisms (SNP) characteristics of Yersinia pestis strains from different natural foci in China.Methods Genome-wide comparison was done to find SNP sites by the Mummer program among 9 Yersinia pestis genome which was downloaded from NCBI.Then 13 genic fragments including 19 SNP sites were amplified by PCR and sequenced in 133 Yersinia pestis strains,and the results were cluster analyzed with the BioNumerics software.Results Three thousand seven hundred and eighty sequence variation sites were found by genome-wide comparison.Using the different combinations of SNP sites,UPGMA cluster analysis revealed obvious geographic regional and eco-aggregation characteristics of Yersinia pestis strains isolated from China.Conclusions As relatively stable genetic markers,SNP can better reflect the genome characteristics of Yersinia pestis in different plague natural foci of China.
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