中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2012年
5期
486-489
,共4页
刘合智%周松%王海峰%白雪薇%胡乐乐%杨顺林%杨晓燕%张懿晖%王俊香
劉閤智%週鬆%王海峰%白雪薇%鬍樂樂%楊順林%楊曉燕%張懿暉%王俊香
류합지%주송%왕해봉%백설미%호악악%양순림%양효연%장의휘%왕준향
酶联免疫吸附测定%耶尔森菌,鼠疫%敏感性与特异性
酶聯免疫吸附測定%耶爾森菌,鼠疫%敏感性與特異性
매련면역흡부측정%야이삼균,서역%민감성여특이성
Enzyme-linked immunosorbent assay%Yersinia pestis%Sensitivity and specificity
目的 观察双单克隆抗体(F1-McAb)夹心酶联免疫试验(DMcAbS-ELISA)快速检测鼠疫F1抗原的敏感性和特异性.方法 采用鼠疫细菌学检验、DMcAbS-ELISA和反向间接血球凝集试验(RIHA)对比检测鼠疫感染鼠和阴性对照鼠脏器标本.结果 共检测225份阴性对照鼠脏器标本,鼠疫细菌学检验、DMcAbS-ELISA和RIHA法检测F1抗原均为阴性.共检测308只鼠疫感染鼠脏器标本,鼠疫细菌学检验、DMcAbS-ELISA、RIHA法阳性率分别为92.21%(284/308)、90.91%(280/308)和89.61%(276/308),3种方法比较,差异无统计学意义(x2=5.65,P>0.05).DMcAbS-ELISA法与鼠疫细菌学检验结果符合率为97.00%[(274+243)/533],Kappa值为0.940;与RIHA法符合率为99.25%[(276+253)/533],Kappa值为0.985.脏器标本F1抗原检测的真实性比较:DMcAbS-ELISA法敏感性为96.48%(274/284),特异性为97.59%(243/249),阳性预测值为97.86%(274/280),阴性预测值为96.05%(243/253),一致性为96.99%11/4×(274/280+274/284+ 243/253+243/249)|,Youden指数为0.9407;RIHA法的敏感性为96.13%(273/284),特异性为98.80%(246/249),阳性预测值为98.91%(273/276),阴性预测值为95.72%(246/257),一致性为97.39%[1/4×(273/276+273/284+246/257+246/249)],Youden指数为0.9492.DMcAbS-ELISA法对鼠疫菌检测灵敏度为2.7×104cfu/ml,RIHA法为2.2×105 cfu/ml;两种方法检测F1抗原灵敏度均为10 μg/L.结论 DMcAbS-ELISA法检测鼠疫F1抗原具有敏感、特异、简便、快速的特点,是有应用价值的鼠疫快速诊断技术.
目的 觀察雙單剋隆抗體(F1-McAb)夾心酶聯免疫試驗(DMcAbS-ELISA)快速檢測鼠疫F1抗原的敏感性和特異性.方法 採用鼠疫細菌學檢驗、DMcAbS-ELISA和反嚮間接血毬凝集試驗(RIHA)對比檢測鼠疫感染鼠和陰性對照鼠髒器標本.結果 共檢測225份陰性對照鼠髒器標本,鼠疫細菌學檢驗、DMcAbS-ELISA和RIHA法檢測F1抗原均為陰性.共檢測308隻鼠疫感染鼠髒器標本,鼠疫細菌學檢驗、DMcAbS-ELISA、RIHA法暘性率分彆為92.21%(284/308)、90.91%(280/308)和89.61%(276/308),3種方法比較,差異無統計學意義(x2=5.65,P>0.05).DMcAbS-ELISA法與鼠疫細菌學檢驗結果符閤率為97.00%[(274+243)/533],Kappa值為0.940;與RIHA法符閤率為99.25%[(276+253)/533],Kappa值為0.985.髒器標本F1抗原檢測的真實性比較:DMcAbS-ELISA法敏感性為96.48%(274/284),特異性為97.59%(243/249),暘性預測值為97.86%(274/280),陰性預測值為96.05%(243/253),一緻性為96.99%11/4×(274/280+274/284+ 243/253+243/249)|,Youden指數為0.9407;RIHA法的敏感性為96.13%(273/284),特異性為98.80%(246/249),暘性預測值為98.91%(273/276),陰性預測值為95.72%(246/257),一緻性為97.39%[1/4×(273/276+273/284+246/257+246/249)],Youden指數為0.9492.DMcAbS-ELISA法對鼠疫菌檢測靈敏度為2.7×104cfu/ml,RIHA法為2.2×105 cfu/ml;兩種方法檢測F1抗原靈敏度均為10 μg/L.結論 DMcAbS-ELISA法檢測鼠疫F1抗原具有敏感、特異、簡便、快速的特點,是有應用價值的鼠疫快速診斷技術.
목적 관찰쌍단극륭항체(F1-McAb)협심매련면역시험(DMcAbS-ELISA)쾌속검측서역F1항원적민감성화특이성.방법 채용서역세균학검험、DMcAbS-ELISA화반향간접혈구응집시험(RIHA)대비검측서역감염서화음성대조서장기표본.결과 공검측225빈음성대조서장기표본,서역세균학검험、DMcAbS-ELISA화RIHA법검측F1항원균위음성.공검측308지서역감염서장기표본,서역세균학검험、DMcAbS-ELISA、RIHA법양성솔분별위92.21%(284/308)、90.91%(280/308)화89.61%(276/308),3충방법비교,차이무통계학의의(x2=5.65,P>0.05).DMcAbS-ELISA법여서역세균학검험결과부합솔위97.00%[(274+243)/533],Kappa치위0.940;여RIHA법부합솔위99.25%[(276+253)/533],Kappa치위0.985.장기표본F1항원검측적진실성비교:DMcAbS-ELISA법민감성위96.48%(274/284),특이성위97.59%(243/249),양성예측치위97.86%(274/280),음성예측치위96.05%(243/253),일치성위96.99%11/4×(274/280+274/284+ 243/253+243/249)|,Youden지수위0.9407;RIHA법적민감성위96.13%(273/284),특이성위98.80%(246/249),양성예측치위98.91%(273/276),음성예측치위95.72%(246/257),일치성위97.39%[1/4×(273/276+273/284+246/257+246/249)],Youden지수위0.9492.DMcAbS-ELISA법대서역균검측령민도위2.7×104cfu/ml,RIHA법위2.2×105 cfu/ml;량충방법검측F1항원령민도균위10 μg/L.결론 DMcAbS-ELISA법검측서역F1항원구유민감、특이、간편、쾌속적특점,시유응용개치적서역쾌속진단기술.
Objective To study the sensitivity and specificity of a double monoclonal antibody sandwich enzyme-linked immunosorbent assay (DMcAbS-ELISA)for the detection of F1 antigen of Yersinia pestis (Y.pestis).Methods Viscera (viz.liver and spleen)specimens of infected mice with virulent Y.pestis and negative control mice were detected by bacteriological test,DMcAbS-ELISA and reverse indirect hemagglutination assay (RIHA) for the F1 antigen.Results The 225 control specimens were all negative tested by plague bacteriology testing,DMcAbS-ELISA and RIHA.A total of 308 plague-infected mouse organ specimens were tested,and the positive detection rate was 92.21% (284/308),90.91%(280/308) and 89.61% (276/308),respectively,with germiculture,DMcAbS-ELISA and RIHA,and the difference was not statistically significant(x2=5.65,P>0.05).The coincidence rate of DMcAbS-ELISA and bacterial culture was 97.00%[(274+243)/533],Kappa =0.940;RIHA in line with the rate was 99.25%[(276+253)/533],Kappa =0.985.Authenticity comparison of F1 antigen detection in viscera specimens:sensitivity,specificity,positive predictive value,negative predictive value,adjusted agreement and Youden's index was 96.48%(274/284),97.59%(243/249),97.86% (274/280),96.05 %(243/253),96.99%[1/4×(274/280+274/284+243/253+243/249)]and 0.9407,respectively,for DMcAbS-ELISA and 96.13%(273/284),98.80%(246/249),98.91%(273/276),95.72%(246/257),97.39%[1/4×(273/276+273/284+246/257±246/249)]and 0.9492,respectively,for RIHA.The detection sensitivity of DMcAbS-ELISA and RIHA was 2.7×104 cfu/ml and 2.2×105 cfu/ml,for Y.pestis,respectively,and was 10 μg/L for F1 antigen.Conclusions DMcAbS-ELISA assay is a sensitive,specific,simple and fast method for detection of the F1 antigen,and it has a potential application value in rapid diagnosis of plague.