中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2013年
4期
362-364
,共3页
马军建%张浩军%张珂%王燕玲%张本忠%王俊玲
馬軍建%張浩軍%張珂%王燕玲%張本忠%王俊玲
마군건%장호군%장가%왕연령%장본충%왕준령
甲状腺功能减退症%精子能动性%精子计数
甲狀腺功能減退癥%精子能動性%精子計數
갑상선공능감퇴증%정자능동성%정자계수
Hypothyroidism%Sperm motility%Sperm count
目的 探讨甲状腺功能减退(甲减)对雄性大鼠精子活动度的影响.方法 健康雄性Wistar大鼠20只,体质量200～240 g,按体质量随机分为2组:生理盐水(对照)组和甲减组(按1 ml/100 g体质量灌服0.1％丙硫氧嘧啶),每组10只,共60d,每3天称体质量.灌胃结束次日处死大鼠,取全血,分离血清.放射免疫法检测血清中甲状腺激素水平:总三碘甲状腺素原氨酸(T3)、总甲状腺素(T4)、促甲状腺激素(TSH).取附睾游离精子,WLJY-9000型伟力彩色精子质量检测系统测定精子运动参数:平均路径速度、直线速度、前向性、侧摆幅度、精子密度、曲线速度、直线性、摆动性、平均移动角度、鞭打频率.结果 灌胃第30、60天甲减组大鼠体质量[(239.00±15.02)、(232.67±17.86)g]均低于对照组[(298.20±12.15)、(344.00±13.73)g,t值分别为7.704、11.380,P均＜0.05].甲状腺激素水平:甲减组大鼠T3[(373.3±101.3)ng/L]、T4[(4.00±0.89)×103 ng/L]水平低于对照组[(1000.0±273.5)ng/L、(44.33±7.84)×103 ng/L,t值分别为5.262、2.520,P均＜0.05],TSH[(5.77±0.89)×103 U/L]水平高于对照组[(1.87±0.70)×103 U/L,t=8.413,P＜0.05].精子参数:甲减组平均路径速度[(27.45±1.59)μm/s]、直线速度[(21.08±1.10)μm/s]、前向性[(70.53±3.48)％]、侧摆幅度[(1.96±0.26)μm]高于对照组[(24.38±2.59)μm/s、(17.99±2.06) μm/s、(65.93±2.71)％、(1.53±0.27)μm,t值分别为2.687、2.404、2.420、3.175,P均＜0.05].甲减组精子密度[(5.07±0.74)109/L]低于对照组[(8.76±1.01) 109/L,t=6.463,P＜ 0.05].甲减组曲线速度[(52.83±5.56)μm/s]、直线性[(38.58±3.41)％]、摆动性[(52.64±3.24)％]、平均移动角度[(64.21±6.71)度/s]、鞭打频率[(8.93±0.62)Hz]与对照组[(49.92±6.43)μm/s、(36.52±2.73)％、(52.49±3.49)％、(62.77±7.34)度/s、(9.32±0.61)Hz]比较差异无统计学意义(t值分别为0.805、1.089、0.037、0.341、1.033,P均＞0.05).结论 甲状腺功能减退可影响雄性大鼠精子活动度,降低精子密度,损害大鼠生殖系统.
目的 探討甲狀腺功能減退(甲減)對雄性大鼠精子活動度的影響.方法 健康雄性Wistar大鼠20隻,體質量200～240 g,按體質量隨機分為2組:生理鹽水(對照)組和甲減組(按1 ml/100 g體質量灌服0.1％丙硫氧嘧啶),每組10隻,共60d,每3天稱體質量.灌胃結束次日處死大鼠,取全血,分離血清.放射免疫法檢測血清中甲狀腺激素水平:總三碘甲狀腺素原氨痠(T3)、總甲狀腺素(T4)、促甲狀腺激素(TSH).取附睪遊離精子,WLJY-9000型偉力綵色精子質量檢測繫統測定精子運動參數:平均路徑速度、直線速度、前嚮性、側襬幅度、精子密度、麯線速度、直線性、襬動性、平均移動角度、鞭打頻率.結果 灌胃第30、60天甲減組大鼠體質量[(239.00±15.02)、(232.67±17.86)g]均低于對照組[(298.20±12.15)、(344.00±13.73)g,t值分彆為7.704、11.380,P均＜0.05].甲狀腺激素水平:甲減組大鼠T3[(373.3±101.3)ng/L]、T4[(4.00±0.89)×103 ng/L]水平低于對照組[(1000.0±273.5)ng/L、(44.33±7.84)×103 ng/L,t值分彆為5.262、2.520,P均＜0.05],TSH[(5.77±0.89)×103 U/L]水平高于對照組[(1.87±0.70)×103 U/L,t=8.413,P＜0.05].精子參數:甲減組平均路徑速度[(27.45±1.59)μm/s]、直線速度[(21.08±1.10)μm/s]、前嚮性[(70.53±3.48)％]、側襬幅度[(1.96±0.26)μm]高于對照組[(24.38±2.59)μm/s、(17.99±2.06) μm/s、(65.93±2.71)％、(1.53±0.27)μm,t值分彆為2.687、2.404、2.420、3.175,P均＜0.05].甲減組精子密度[(5.07±0.74)109/L]低于對照組[(8.76±1.01) 109/L,t=6.463,P＜ 0.05].甲減組麯線速度[(52.83±5.56)μm/s]、直線性[(38.58±3.41)％]、襬動性[(52.64±3.24)％]、平均移動角度[(64.21±6.71)度/s]、鞭打頻率[(8.93±0.62)Hz]與對照組[(49.92±6.43)μm/s、(36.52±2.73)％、(52.49±3.49)％、(62.77±7.34)度/s、(9.32±0.61)Hz]比較差異無統計學意義(t值分彆為0.805、1.089、0.037、0.341、1.033,P均＞0.05).結論 甲狀腺功能減退可影響雄性大鼠精子活動度,降低精子密度,損害大鼠生殖繫統.
목적 탐토갑상선공능감퇴(갑감)대웅성대서정자활동도적영향.방법 건강웅성Wistar대서20지,체질량200～240 g,안체질량수궤분위2조:생리염수(대조)조화갑감조(안1 ml/100 g체질량관복0.1％병류양밀정),매조10지,공60d,매3천칭체질량.관위결속차일처사대서,취전혈,분리혈청.방사면역법검측혈청중갑상선격소수평:총삼전갑상선소원안산(T3)、총갑상선소(T4)、촉갑상선격소(TSH).취부고유리정자,WLJY-9000형위력채색정자질량검측계통측정정자운동삼수:평균로경속도、직선속도、전향성、측파폭도、정자밀도、곡선속도、직선성、파동성、평균이동각도、편타빈솔.결과 관위제30、60천갑감조대서체질량[(239.00±15.02)、(232.67±17.86)g]균저우대조조[(298.20±12.15)、(344.00±13.73)g,t치분별위7.704、11.380,P균＜0.05].갑상선격소수평:갑감조대서T3[(373.3±101.3)ng/L]、T4[(4.00±0.89)×103 ng/L]수평저우대조조[(1000.0±273.5)ng/L、(44.33±7.84)×103 ng/L,t치분별위5.262、2.520,P균＜0.05],TSH[(5.77±0.89)×103 U/L]수평고우대조조[(1.87±0.70)×103 U/L,t=8.413,P＜0.05].정자삼수:갑감조평균로경속도[(27.45±1.59)μm/s]、직선속도[(21.08±1.10)μm/s]、전향성[(70.53±3.48)％]、측파폭도[(1.96±0.26)μm]고우대조조[(24.38±2.59)μm/s、(17.99±2.06) μm/s、(65.93±2.71)％、(1.53±0.27)μm,t치분별위2.687、2.404、2.420、3.175,P균＜0.05].갑감조정자밀도[(5.07±0.74)109/L]저우대조조[(8.76±1.01) 109/L,t=6.463,P＜ 0.05].갑감조곡선속도[(52.83±5.56)μm/s]、직선성[(38.58±3.41)％]、파동성[(52.64±3.24)％]、평균이동각도[(64.21±6.71)도/s]、편타빈솔[(8.93±0.62)Hz]여대조조[(49.92±6.43)μm/s、(36.52±2.73)％、(52.49±3.49)％、(62.77±7.34)도/s、(9.32±0.61)Hz]비교차이무통계학의의(t치분별위0.805、1.089、0.037、0.341、1.033,P균＞0.05).결론 갑상선공능감퇴가영향웅성대서정자활동도,강저정자밀도,손해대서생식계통.
Objective To study the impact of hypothyroidism on sperm motility in male rat.Methods According to body weight,20 male Wistar rats were randomly divided into control group and hypothyroidism group (1 ml/100 g/day,0.1％ propylthiouracil by intragastric administration for 60 days) 10 rats in eachgroup.Body weight of these rats was observed every 3 days.After the last intragastric administration,all rats were killed.The levels of thyroid hormones [total triiodothyronine (T3),total thyroxine (T4),and thyroid stimulating hormone (TSH)] were measured by radioimmunoassay.Sperm motility parameters[average path velocity(VAP),straight fine velocity (VSL),straightness (STR),amplitude of lateral head displacement (ALH),sperm density(p),curvilinear velocity (VCL),linearity(LIN),wobble (WOB),mean angular deviation (MAD) and beat cross frequency (BCF)] were measured by a WLJY-9000 color-detection system.Results Compared with the control groups[(298.20 ± 12.15) g,(344.00 ± 13.73)g],the weights of hypothyroidism group of the 30 days[(239.00 ± 15.02) g] and the 60 days [(232.67 ± 17.86)g] were significantly lower(t =7.704,11.380,all P ＜ 0.05).The levels of T3[(373.3 ± 101.3) ng/L] and T4 [(4.00 ± 0.89) × 103 ng/ml] of hypothyroidism group were significantly decreased compared with that of the control groups [(1000.0 ± 273.5)ng/L,(44.33 ± 7.84)× 103 ng/L,t =5.262,12.520,all P ＜ 0.05].Level of TSH[(5.77 ± 0.89) × 103 U/L] of hypothyroidism group was significantly increased compared with that of the control group[(1.87 ± 0.70) × 103 U/L,t =8.413,P ＜ 0.05].Values of VAP[(27.45 ± 1.59)μm/s],VSL [(21.08 ± 1.10)μm/s],STR[(70.53 ± 3.48)％] and ALH[(1.96 ± 0.26)μm] of hypothyroidism group were significantly increased compared with that of the control groups[(24.38 ± 2.59)μm/s,(17.99±2.06)μm/s,(65.93 ± 2.71)％,(1.53 ± 0.27)μm,t =2.687,2.404,2.420,3.175,all P ＜ 0.05].p[(5.07 ± 0.74)109/L] of hypothyroidism group was significantly decreased compared with that of the control group [(8.76 ± 1.01)109/L,t =6.463,P ＜ 0.05].VCL[(52.83 ± 5.56)μm/s],LIN[(38.58 ± 3.41)％],WOB[(52.64 ± 3.24)％],MAD [(64.21 ± 6.71) radian/s] and BCF [(8.93 ± 0.62) Hz] of hypothyroidism group were not significantly different compared with that of the control groups[(49.92 ± 6.43) μm/s,(36.52 ± 2.73)％,(52.49 ± 3.49)％,(62.77 ± 7.34)radia/s,(9.32 ± 0.61)Hz,t =0.805,1.089,0.037,0.341,1.033,all P ＞ 0.05].Conclusion Hypothyroidism can affect sperm activity in male rats,decrease sperm density and cause damage to the reproductive system.
