中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2013年
4期
453-457
,共5页
色谱法,高压液相%儿茶素%生物碱类%正交试验
色譜法,高壓液相%兒茶素%生物堿類%正交試驗
색보법,고압액상%인다소%생물감류%정교시험
Chromatography,high pressure liquid%Catechin%Alkaloids%Orthogonal test
目的 建立测定砖茶中儿茶素和生物碱的色谱分析方法,同时寻找出砖茶中儿茶素和生物碱的较优提取条件.方法 采用高效液相色谱法分离检测砖茶中儿茶素和生物碱,并进行回收率、精密度、稳定性实验.分别采用水、乙醇作为提取溶剂对砖茶中儿茶素和生物碱提取情况进行正交实验,再比较水和醇的提取效果,以获得提取砖茶中儿茶素和生物碱的较优提取条件.结果 高效液相色谱法可同时分离并检测出砖茶中儿茶素、表儿茶素、表没食子儿茶素、表没食子儿茶素没食子酸酯、没食子儿茶素、表儿茶素没食子酸酯6种儿茶素以及咖啡碱、可可碱2种生物碱.各组分的峰面积和浓度之间具有良好的线性关系,决定系数(R2)范围为0.9990 ～ 0.9999；加标回收率范围为83.78％～ 106.35％.精密度较好[峰面积相对标准偏差(RSD)范围为0.50％～1.51％].砖茶中儿茶素和生物碱较优提取条件为:乙醇浓度80％、料液比1∶10、温度80℃、时间30 min.结论 乙醇作为提取剂的高效液相色谱法的较优提取条件,对砖茶中6种儿茶素和2种生物碱分离度较好,定性准确,快速灵敏.
目的 建立測定磚茶中兒茶素和生物堿的色譜分析方法,同時尋找齣磚茶中兒茶素和生物堿的較優提取條件.方法 採用高效液相色譜法分離檢測磚茶中兒茶素和生物堿,併進行迴收率、精密度、穩定性實驗.分彆採用水、乙醇作為提取溶劑對磚茶中兒茶素和生物堿提取情況進行正交實驗,再比較水和醇的提取效果,以穫得提取磚茶中兒茶素和生物堿的較優提取條件.結果 高效液相色譜法可同時分離併檢測齣磚茶中兒茶素、錶兒茶素、錶沒食子兒茶素、錶沒食子兒茶素沒食子痠酯、沒食子兒茶素、錶兒茶素沒食子痠酯6種兒茶素以及咖啡堿、可可堿2種生物堿.各組分的峰麵積和濃度之間具有良好的線性關繫,決定繫數(R2)範圍為0.9990 ～ 0.9999；加標迴收率範圍為83.78％～ 106.35％.精密度較好[峰麵積相對標準偏差(RSD)範圍為0.50％～1.51％].磚茶中兒茶素和生物堿較優提取條件為:乙醇濃度80％、料液比1∶10、溫度80℃、時間30 min.結論 乙醇作為提取劑的高效液相色譜法的較優提取條件,對磚茶中6種兒茶素和2種生物堿分離度較好,定性準確,快速靈敏.
목적 건립측정전다중인다소화생물감적색보분석방법,동시심조출전다중인다소화생물감적교우제취조건.방법 채용고효액상색보법분리검측전다중인다소화생물감,병진행회수솔、정밀도、은정성실험.분별채용수、을순작위제취용제대전다중인다소화생물감제취정황진행정교실험,재비교수화순적제취효과,이획득제취전다중인다소화생물감적교우제취조건.결과 고효액상색보법가동시분리병검측출전다중인다소、표인다소、표몰식자인다소、표몰식자인다소몰식자산지、몰식자인다소、표인다소몰식자산지6충인다소이급가배감、가가감2충생물감.각조분적봉면적화농도지간구유량호적선성관계,결정계수(R2)범위위0.9990 ～ 0.9999；가표회수솔범위위83.78％～ 106.35％.정밀도교호[봉면적상대표준편차(RSD)범위위0.50％～1.51％].전다중인다소화생물감교우제취조건위:을순농도80％、료액비1∶10、온도80℃、시간30 min.결론 을순작위제취제적고효액상색보법적교우제취조건,대전다중6충인다소화2충생물감분리도교호,정성준학,쾌속령민.
Objective To develop a method for determination of catechins and alkaloids in brick-tea by high performance liquid chromatography(HPLC) with diode array detection(DAD),and to explore optimum extraction conditions for catechins and alkaloids components in brick-tea.Methods Catechins and alkaloids were separated and detected by HPLC.Orthogonal experiment and paired t test was carried out to compare the effect of water and alcohol as extraction solvents and to obtain optimum extraction conditions for extracting catechins and alkaloids components in brick-tea.Results Six kinds of catechins of catechin,epicatechin,gallocatechin,epigallocatechin,epicatechin gallate,epigallocatechin gallate and two kinds of alkaloids of caffeine and theobromine were separated and detected simultaneously.Calibration curves between peak areas and concentration of each component in bricktea were linear within a suitable concentration range,and coefficients of determination (R2) were between 0.9990-0.9999; spiked recoveries were from 83.78％ to 106.35％,and relative standard deviations(RSD) were between 0.50％-1.51％.The optimum extraction condition for catechins and alkaloids was 80％ ethanol,solid-liquid ratio of 1:10,temperature 80 ℃ and extraction time 30 min.Conclusion Alcohol as extraction solvents with optimal combination on HPLC,six kinds of catechins and two kinds of alkaloids are separated accurately,qualitatively,rapidly and sensitively.
