中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2014年
1期
7-10
,共4页
甲基化%砷%数据收集
甲基化%砷%數據收集
갑기화%신%수거수집
Methylation%Arsenic%Data collection
目的 探讨慢性高砷水暴露人群尿砷甲基化代谢的影响因素.方法 选取内蒙古自治区1个饮水型地方性砷中毒(地砷病)病区村(水砷0.167 mg/L)居民作为观察对象,其中男73人,女72人,采集晨尿,同时测量身高和体质量,计算体质指数(BMI),并问卷调查吸烟和饮酒情况.采用高效液相色谱-氢化物发生-原子荧光光谱法测定尿样中无机砷(iAs)、一甲基砷(MMA)、二甲基砷(DMA)含量.以iAs、MMA、DMA之和表示近似总砷(T-As)水平,以iAs/T-As、MMA/T-As、DMA/T-As分别计算iAs、MMA、DMA占总砷的百分比(iAs％、MMA％、DMA％);以MMA/iAs、DMA/MMA分别计算一甲基化率(PMI)和二甲基化率(SMI).结果 女性SMI水平高于男性,二者比较差异有统计学意义[中位数(M值)分别为4.18、3.38,x2=11.219,P＜ 0.05].女性MMA％水平低于男性,二者比较差异有统计学意义(M值分别为15.19、18.06,x2-9.272,P＜0.05).女性DMA％水平高于男性,二者比较差异有统计学意义(M值分别为65.61、59.47,x2=11.728,P＜0.05).吸烟、饮酒、BMI均未见明显影响各砷形态指标(P均＞ 0.05).BMI与MMA％之间呈负相关关系,相关系数(r)为-0.17 (P＜0.05).结论 在相同砷暴露情况下,女性砷的甲基化能力高于男性.随着BMI指数降低砷的甲基化能力增强.
目的 探討慢性高砷水暴露人群尿砷甲基化代謝的影響因素.方法 選取內矇古自治區1箇飲水型地方性砷中毒(地砷病)病區村(水砷0.167 mg/L)居民作為觀察對象,其中男73人,女72人,採集晨尿,同時測量身高和體質量,計算體質指數(BMI),併問捲調查吸煙和飲酒情況.採用高效液相色譜-氫化物髮生-原子熒光光譜法測定尿樣中無機砷(iAs)、一甲基砷(MMA)、二甲基砷(DMA)含量.以iAs、MMA、DMA之和錶示近似總砷(T-As)水平,以iAs/T-As、MMA/T-As、DMA/T-As分彆計算iAs、MMA、DMA佔總砷的百分比(iAs％、MMA％、DMA％);以MMA/iAs、DMA/MMA分彆計算一甲基化率(PMI)和二甲基化率(SMI).結果 女性SMI水平高于男性,二者比較差異有統計學意義[中位數(M值)分彆為4.18、3.38,x2=11.219,P＜ 0.05].女性MMA％水平低于男性,二者比較差異有統計學意義(M值分彆為15.19、18.06,x2-9.272,P＜0.05).女性DMA％水平高于男性,二者比較差異有統計學意義(M值分彆為65.61、59.47,x2=11.728,P＜0.05).吸煙、飲酒、BMI均未見明顯影響各砷形態指標(P均＞ 0.05).BMI與MMA％之間呈負相關關繫,相關繫數(r)為-0.17 (P＜0.05).結論 在相同砷暴露情況下,女性砷的甲基化能力高于男性.隨著BMI指數降低砷的甲基化能力增彊.
목적 탐토만성고신수폭로인군뇨신갑기화대사적영향인소.방법 선취내몽고자치구1개음수형지방성신중독(지신병)병구촌(수신0.167 mg/L)거민작위관찰대상,기중남73인,녀72인,채집신뇨,동시측량신고화체질량,계산체질지수(BMI),병문권조사흡연화음주정황.채용고효액상색보-경화물발생-원자형광광보법측정뇨양중무궤신(iAs)、일갑기신(MMA)、이갑기신(DMA)함량.이iAs、MMA、DMA지화표시근사총신(T-As)수평,이iAs/T-As、MMA/T-As、DMA/T-As분별계산iAs、MMA、DMA점총신적백분비(iAs％、MMA％、DMA％);이MMA/iAs、DMA/MMA분별계산일갑기화솔(PMI)화이갑기화솔(SMI).결과 녀성SMI수평고우남성,이자비교차이유통계학의의[중위수(M치)분별위4.18、3.38,x2=11.219,P＜ 0.05].녀성MMA％수평저우남성,이자비교차이유통계학의의(M치분별위15.19、18.06,x2-9.272,P＜0.05).녀성DMA％수평고우남성,이자비교차이유통계학의의(M치분별위65.61、59.47,x2=11.728,P＜0.05).흡연、음주、BMI균미견명현영향각신형태지표(P균＞ 0.05).BMI여MMA％지간정부상관관계,상관계수(r)위-0.17 (P＜0.05).결론 재상동신폭로정황하,녀성신적갑기화능력고우남성.수착BMI지수강저신적갑기화능력증강.
Objective To explore the factors affecting urinary arsenic methylated metabolites in population exposed to elevated arsenic through drinking water.Methods Residents,including 73 males and 72 females,in a endemic village located in Inner Mongolia Autonomous Region where the concentration of arsenic in drinking water was 0.167 mg/L,were included in the study; moming urine samples were collected; individual height and weight were measured which could be used to calculate body mass index(BM庆) ; and smoking and drinking habit was investigated by a questionnaire survey.Urinary concentrations of inorganic arsenic (iAs),monomethylarsenic acid(MMA) and dimethylarsenic acid(DMA) were determined using high performance liquid chromatography hydride generation atomic fluorescence spectrometry (HPLC-HG-AFC).Total arsenic (T-As) level was calculated by the sum of iAs,MMA and DMA; iAs％,MMA％ and DMA％ was calculated based on iAs/T-As,MMA/T-As and DMA/T-As.One methylation ratio(PMI) and dimethyl rate(SMI) were calculated by MMA/iAs and DMA/MMA.Results SMI level of female was significantly higher than that of male [median (M) =4.18,3.38,x2 =11.219,P ＜ 0.05] ; MMA％ of female was significantly lower than that of male(M =15.1 9,18.06,x2 =9.272,P ＜ 0.05) ; DMA％ of female was significantly higher than that of male (M =65.61,59.47,x2 =11.728,P ＜ 0.05).Various forms of arsenic indexes were not affected by smoking,alcohol consumption and BMI(P ＞ 0.05).BMI was negatively correlated with MMA％,with correlation coefficient (r)-0.17 (P ＜ 0.05).Conclusions Under the same condition of arsenic exposure,arsenic methylation capacity of women is higher than that of men.Lower BMI is significantly associated with higher methylation capability of arsenic,but this correlation may be affected by other confounding factors.
