CAJ | 학술논문

目的观察慢性砷暴露对雌鼠子宫和卵巢组织雌激素受体结合位点相关抗原9(Ebag9)和雌激.素反应指蛋白(efp)mRNA表达的影响.方法将50只雌性Wistar大鼠按体质量随机分成5组,每组10只,分别饮用含砷量(三氧化二砷,As2O3)为0.00(对照)、0.05、0.10、0.20、0.40 mg/L的水溶液,31周后通过实时定量PCR方法检测大鼠子宫和卵巢组织中Ebag9和efp mRNA的表达.结果对照、0.05、0.10、0.20、0.40 mg/L组大鼠子宫组织Ebag9和efp mRNA表达量分别为0.761±0.178、0.521±0.130、0.544±0.035、0.525±0.198、0.498±0.240和0.795±0.171、0.874±0.077、0.797±0.066、0.796±0.040、0.832±0.096.与对照组比较,染砷各组Ebag9 mRNA表达呈降低趋势,但差异无统计学意义(P均＞0.05),其中0.40 mg/L组P值为0.055;与对照组比较,染砷各组efp mRNA表达呈升高趋势,但差异无统计学意义(P均＞0.05).对照、0.05、0.10、0.20、0.40 mg/L组大鼠卵巢组织Ebag9和efp mRNA表达量分别为0.702±0.484、0.719±0.336、0.693±0.095、0.706±0.055、0.728±0.073和0.924±0.061、1.009±0.034、0.930±0.085、0.929±0.068、1.012±0.101. 0.05、0.20、0.40 mg/L组Ebag9 mRNA表达呈升高趋势,但与对照组比较,差异无统计学意义(P均＞0.05);染砷各组efp mRNA表达均呈升高趋势,其中0.05、0.40 mg/L组与对照组比较,差异有统计学意义(P均＜0.05).结论慢性砷暴露可引起雌性大鼠卵巢组织efp mRNA表达改变.砷可能对雌性大鼠卵巢组织存在内分泌干扰效应.
목적 관찰만성신폭로대자서자궁화란소조직자격소수체결합위점상관항원9(Ebag9)화자격.소반응지단백(efp)mRNA표체적영향.방법 장50지자성Wistar대서안체질량수궤분성5조,매조10지,분별음용함신량(삼양화이신,As2O3)위0.00(대조)、0.05、0.10、0.20、0.40 mg/L적수용액,31주후통과실시정량PCR방법검측대서자궁화란소조직중Ebag9화efp mRNA적표체.결과 대조、0.05、0.10、0.20、0.40 mg/L조대서자궁조직Ebag9화efp mRNA표체량분별위0.761±0.178、0.521±0.130、0.544±0.035、0.525±0.198、0.498±0.240화0.795±0.171、0.874±0.077、0.797±0.066、0.796±0.040、0.832±0.096.여대조조비교,염신각조Ebag9 mRNA표체정강저추세,단차이무통계학의의(P균＞0.05),기중0.40 mg/L조P치위0.055;여대조조비교,염신각조efp mRNA표체정승고추세,단차이무통계학의의(P균＞0.05).대조、0.05、0.10、0.20、0.40 mg/L조대서란소조직Ebag9화efp mRNA표체량분별위0.702±0.484、0.719±0.336、0.693±0.095、0.706±0.055、0.728±0.073화0.924±0.061、1.009±0.034、0.930±0.085、0.929±0.068、1.012±0.101. 0.05、0.20、0.40 mg/L조Ebag9 mRNA표체정승고추세,단여대조조비교,차이무통계학의의(P균＞0.05);염신각조efp mRNA표체균정승고추세,기중0.05、0.40 mg/L조여대조조비교,차이유통계학의의(P균＜0.05).결론 만성신폭로가인기자성대서란소조직efp mRNA표체개변.신가능대자성대서란소조직존재내분비간우효응.
Objective To observe the effects of chronic arsenic exposure on mRNA expression of estrogen receptor-binding fragment-associated gene 9 (Ebag9) and estrogen-responsive finger protein (efp) in uterus and ovary of female rats.Methods Fifty female Wistar rats were randomly divided into five groups according to arsenic (As2O3) concentrations given through drinking-water:0.00 (control),0.05,0.10,0.20,0.40 mg/L arsenic exposure groups and real-time RCR (RT-PCR) was used to detect the mRNA expression of Ebag9 and efp in uterus and ovary tissue at the 31 weeks of experiment.Results The mRNA expression levels of Ebag9 and efp of the 0.00,0.05,0.10,0.20,0.40 mg/L arsenic exposure groups were respectively as follows:0.761 ± 0.178,0.521 ± 0.130,0.544 ± 0.035,0.525 ± 0.198,0.498 ± 0.240 and 0.795 ± 0.171,0.874 ± 0.077,0.797 ± 0.066,0.796 ± 0.040,0.832 ± 0.096.Compared with control group,a decreased tendency was observed in Ebag9 mRNA level(with P value 0.055 in 0.40 mg/L arsenic exposure group) and increased tendency in efp mRNA level in experimental groups (all P ＞ 0.05).The mRNA expression levels of Ebag9 and efp in ovary of the five groups were by turns:0.702 ± 0.484,0.719 ± 0.336,0.693 ± 0.095,0.706 ± 0.055,0.728 ± 0.073 and 0.924 ± 0.061,1.009 ± 0.034,0.930 ± 0.085,0.929 ± 0.068,1.012 ± 0.101.Compared with control group,the expression level of Ebag9 mRNA showed a increased tendency in 0.05,0.20,0.40 mg/L arsenic exposure groups(all P ＞ 0.05).The efp mRNA level increased in experimental groups,with significant difference in 0.05,0.40 mg/L groups (all P ＜ 0.05).Conclusions The expression of efp mRNA has changed in ovary of female rats exposed to chronic arsenic.Arsenic may act as an environmental endocrine disruptor to exert its effect.