CAJ | 학술논문

目的分离鉴定内蒙古乌兰察布市人间布鲁杆菌病(简称布病)病原菌,为乌兰察布市布病防控提供参考.方法采集布病患者的血液样本,用双相培养瓶进行布鲁杆菌分离培养,获得4株可疑布鲁杆菌菌株.以牛种标准菌株544A、羊种标准菌株16M、猪种菌标准株1330S为阳性对照,以大肠埃希菌O:157为阴性对照,用传统的布鲁杆菌鉴定方法、布鲁杆菌属特异性聚合酶链式反应(BCSP31-PCR)和多重荧光定量PCR方法进行待测菌株种型鉴定.结果本次试验分离到4株可疑布鲁杆菌菌株,经传统方法鉴定为布鲁杆菌,BCSP31-PCR和多重荧光定量PCR扩增均为阳性,其中3株为羊3型,1株为羊1型.结论从乌兰察布市布病患者体内成功地分离出布病病原菌,证实了乌兰察布市人间布病的主要传染源为染疫的羊.
목적 분리감정내몽고오란찰포시인간포로간균병(간칭포병)병원균,위오란찰포시포병방공제공삼고.방법 채집포병환자적혈액양본,용쌍상배양병진행포로간균분리배양,획득4주가의포로간균균주.이우충표준균주544A、양충표준균주16M、저충균표준주1330S위양성대조,이대장애희균O:157위음성대조,용전통적포로간균감정방법、포로간균속특이성취합매련식반응(BCSP31-PCR)화다중형광정량PCR방법진행대측균주충형감정.결과 본차시험분리도4주가의포로간균균주,경전통방법감정위포로간균,BCSP31-PCR화다중형광정량PCR확증균위양성,기중3주위양3형,1주위양1형.결론 종오란찰포시포병환자체내성공지분리출포병병원균,증실료오란찰포시인간포병적주요전염원위염역적양.
Objectve To isolate and identify human brucellosis pathogens in Ulanqab,and to provide a reference for prevention and control of brucellosis.Methods Blood samples of patients with brucellosis were collected; Brucella was isolated by two phase culture bottles.Three standard Brucella strains (Brucella aborttus 544A,Brucella melitensis 16M and Brucella suis 1330S) were used as positive controls and Escherichia coli O:157 was used as a negative control,and 4 Brucella strains were tested.Traditional Brucella identification method,genus specific Brucella surface protein 31 PCR(BCSP31-PCR) and multiplex fluorescence quantitative PCR were applied for isotype identification.Results Four Brucella strains were isolated,which were identified as Brucella via traditional identification method; both BCSP31-PCR amplification and multiplex quantitative PCR were positive,including 3 strains of melitensis 3 type and 1 type strain of melitensis 1 type were identified.Conclusion Brucellosis bacteria have been isolated,which has confirmed Brucella melitensis is the main source of infection for human brucellosis.