CAJ | 학술논문

目的探讨饮茶型氟中毒病区不同民族人群血清甲状旁腺激素(PTH)含量及其基因多态性的种族分布差异.方法在内蒙古、青海和新疆的饮茶型氟中毒重点病区乡,对16岁以上人群进行问卷调查,内容包括基本信息、膳食调查和总氟摄入情况,并采集外周静脉血,采用质谱法进行PTH基因单核苷酸多态性(SNP)分型,采用放射免疫法检测血清中PTH含量.结果共有1 175位受试者入选,其中蒙古族308位、哈萨克族295位、俄罗斯族155位、藏族381位、汉族36位.受试者全血的PIH基因分为AA、AG、GG基因型,基因分布符合Hardy-weinberg平衡.AA、AG、GG 3种基因型分布频率,蒙古族分别为0.6％ (2/308)、20.2％ (62/308)、79.2％ (244/308);哈萨克族分别为4.4％(13/295)、25.1％ (74/295)、70.5％(208/295);俄罗斯族分别为2.0％(3/155)、33.5％(52/155)、64.5％(100/155);藏族分别为2.4％(9/381)、31.2％(119/381)、66.4％(253/381);汉族分别为5.6％(2/36)、13.8％(5/36)、80.6％(29/36).不同民族之间基因型分布频率比较差异有统计学意义(x2=28.86,P＜0.05).共检测1 009份血清,其中蒙古族245份、哈萨克族282份、俄罗斯族105份、藏族345份、汉族32份,血清PIH含量分别为(16.36±2.48)、(14.30±3.76)、(15.22±3.92)、(13.26±3.66)、(15.42±1.12)mg/L,各民族间比较差异有统计学意义(F=31.25,P＜0.05).AA、AG和GG 3种基因型携带者(分别为27、270和712例)血清PTH含量分别为(13.75±2.05)、(14.30±3.80)、(14.67±3.65) mg/L,组间比较,差异无统计学意义(F=1.72,P＞0.05).结论在饮茶型氟中毒病区不同民族人群血清中PTH含量不同,PTH基因多态性分布存在种族差异,但携带不同PTH基因型人群的血清PTH水平未见明显的差别.
목적 탐토음다형불중독병구불동민족인군혈청갑상방선격소(PTH)함량급기기인다태성적충족분포차이.방법 재내몽고、청해화신강적음다형불중독중점병구향,대16세이상인군진행문권조사,내용포괄기본신식、선식조사화총불섭입정황,병채집외주정맥혈,채용질보법진행PTH기인단핵감산다태성(SNP)분형,채용방사면역법검측혈청중PTH함량.결과 공유1 175위수시자입선,기중몽고족308위、합살극족295위、아라사족155위、장족381위、한족36위.수시자전혈적PIH기인분위AA、AG、GG기인형,기인분포부합Hardy-weinberg평형.AA、AG、GG 3충기인형분포빈솔,몽고족분별위0.6％ (2/308)、20.2％ (62/308)、79.2％ (244/308);합살극족분별위4.4％(13/295)、25.1％ (74/295)、70.5％(208/295);아라사족분별위2.0％(3/155)、33.5％(52/155)、64.5％(100/155);장족분별위2.4％(9/381)、31.2％(119/381)、66.4％(253/381);한족분별위5.6％(2/36)、13.8％(5/36)、80.6％(29/36).불동민족지간기인형분포빈솔비교차이유통계학의의(x2=28.86,P＜0.05).공검측1 009빈혈청,기중몽고족245빈、합살극족282빈、아라사족105빈、장족345빈、한족32빈,혈청PIH함량분별위(16.36±2.48)、(14.30±3.76)、(15.22±3.92)、(13.26±3.66)、(15.42±1.12)mg/L,각민족간비교차이유통계학의의(F=31.25,P＜0.05).AA、AG화GG 3충기인형휴대자(분별위27、270화712례)혈청PTH함량분별위(13.75±2.05)、(14.30±3.80)、(14.67±3.65) mg/L,조간비교,차이무통계학의의(F=1.72,P＞0.05).결론 재음다형불중독병구불동민족인군혈청중PTH함량불동,PTH기인다태성분포존재충족차이,단휴대불동PTH기인형인군적혈청PTH수평미견명현적차별.
Objective In this study,the differences of serum parathyroid hormone(PTH) and its gene polymorphism in different ethnic groups in drinking brick-tea-borne endemic fluorosis areas were investigated.Methods Inhabitants over the age of 16 years old in Inner Mongolia,Qinghai and Xinjiang were investigated.The questionnaire survey included basic information,dietary survey and total fluoride intake,and peripheral venous blood was collected.The PTH gene single nucleotide polymorphism (SNP) genotyping was determined using mass spectrometry,and PTH levels in serum were determined by radioimmunoassay.Results In the 1 175 copies of whole blood samples (308 Mongolians,295 Kazakhs,155 Ethnic Russians,381 Tibetans and 36 Han people),PTH genes were typed into AA,AG and GG genotypes,and gene distribution met Hardy-weinberg equilibrium.The frequencies of genotypes AA,AG and GG in Mongolian were 0.6％ (2/308),20.2％ (62/308) and 79.2％ (244/308); the frequencies of the three genotypes in kazakh were 4.4％(13/295),25.1％(74/295) and 70.5％ (208/295); the frequencies of the three genotypes in Russians were 2.0％ (3/155),33.5％(52/155) and 64.5％ (100/155); the frequencies of the three genotypes in Tibetan were 2.4％ (9/381),31.2％ (119/381) and 66.4％ (253/381) ; the frequencies of genotypes in Han people were 5.6％(2/36),13.8％(5/36) and 80.6％(29/36).The differences of the three genotype frequencies between different ethnic groups were statistically significant(x2 =28.86,P ＜ 0.05).One thousand and nine peripheral venous blood were detected.The levels of PTH in the five ethnic groups(245,282,105,345 and 32) were (16.36 ± 2.48),(14.30 + 3.76),(15.22 ± 3.92),(13.26 ± 3.66) and (15.42 ± 1.12)mg/L,respectively,and the difference between various ethnic groups was statistically significant(F =31.25,P ＜ 0.05).Serum PTH levels in different genotypes(AA:27,AG:270,GG:712) were (13.75 ± 2.05),(14.30 ± 3.80) and (14.67 + 3.65)mg/L,and the difference was not statistically significant between the groups (F =1.72,P ＞ 0.05).Conclusion PTH gene polymorphisms have racial differences in different ethnic groups,and serum PTH levels are different,but the levels of serum PTH are not different in people with different genotypes.