中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2014年
2期
138-140
,共3页
王世君%夏曙华%余司文%徐静峰%喻茂娟%杨月婷
王世君%夏曙華%餘司文%徐靜峰%喻茂娟%楊月婷
왕세군%하서화%여사문%서정봉%유무연%양월정
氟中毒,牙%煤%雌二醇%鼠
氟中毒,牙%煤%雌二醇%鼠
불중독,아%매%자이순%서
Fluorosis,dental%Coal%Estradiol%Rats
目的 了解食用煤氟污染的粮食对雌鼠阴道开张日龄、动情周期及生殖内分泌功能的影响.方法 选用90只断乳2周、体质量60～ 80g的SD雌性大鼠,将大鼠按体质量采用随机数字表法分为对照组、中氟组、高氟组,每组30只.各染氟组通过喂饲含不同比例的燃煤型氟中毒病区煤烘烤玉米(含氟量分别为47.8、96.0 mg/kg)复制燃煤型氟中毒模型,对照组的玉米饲料来自于非病区(含氟量为5.2 mg/kg).分别于染氟60、120、180 d后,分3批动情期处死大鼠,每组每批10只.喂养初期,每天早上8:00观察并记录雌鼠阴道开张情况.处死前连续10 d,雌鼠行阴道脱落细胞涂片,HE染色后判别动情周期.于动情期股动脉取血检测雌激素(E2)水平.结果 高氟组雌鼠阴道开张日龄[(49.0±4.5)d]长于对照组、中氟组[(45.4±2.3)、(46.7±1.9)d,P均＜0.05].染氟120 d,高氟组的雌鼠动情周期[(5.8±0.8)d]长于对照组、中氟组[(4.4±0.9)、(4.9±0.7)d,P均＜0.05].染氟180 d,对照组、中氟组、高氟组的雌鼠动情周期[(4.3±0.7)、(5.4±1.1)、(6.7±0.9)d]依次延长(P均＜ 0.05).在高氟组,染氟180 d雌鼠的动情周期[(6.7±0.9)d]高于染氟60、120 d[(5.2±1.1)、(5.8±0.8)d,P均＜0.05].染氟60 d时,中、高氟组雌鼠E2含量[(438.75±71.80)、(456.25±57.80) ng/L]均高于对照组[(301.25±94.17)ng/L,P均＜0.05],随染氟剂量增加而逐渐升高;仅高氟组在180 d E2水平[(275.00±127.83)ng/L]低于60、120 d[(456.25±57.80)、(392.50±102.50)ng/L,P均＜0.05].结论 燃煤型氟中毒可导致雌鼠开张日龄和动情周期延长及生殖内分泌水平改变,且染氟剂量越多、染毒时间越长,影响越重.
目的 瞭解食用煤氟汙染的糧食對雌鼠陰道開張日齡、動情週期及生殖內分泌功能的影響.方法 選用90隻斷乳2週、體質量60～ 80g的SD雌性大鼠,將大鼠按體質量採用隨機數字錶法分為對照組、中氟組、高氟組,每組30隻.各染氟組通過餵飼含不同比例的燃煤型氟中毒病區煤烘烤玉米(含氟量分彆為47.8、96.0 mg/kg)複製燃煤型氟中毒模型,對照組的玉米飼料來自于非病區(含氟量為5.2 mg/kg).分彆于染氟60、120、180 d後,分3批動情期處死大鼠,每組每批10隻.餵養初期,每天早上8:00觀察併記錄雌鼠陰道開張情況.處死前連續10 d,雌鼠行陰道脫落細胞塗片,HE染色後判彆動情週期.于動情期股動脈取血檢測雌激素(E2)水平.結果 高氟組雌鼠陰道開張日齡[(49.0±4.5)d]長于對照組、中氟組[(45.4±2.3)、(46.7±1.9)d,P均＜0.05].染氟120 d,高氟組的雌鼠動情週期[(5.8±0.8)d]長于對照組、中氟組[(4.4±0.9)、(4.9±0.7)d,P均＜0.05].染氟180 d,對照組、中氟組、高氟組的雌鼠動情週期[(4.3±0.7)、(5.4±1.1)、(6.7±0.9)d]依次延長(P均＜ 0.05).在高氟組,染氟180 d雌鼠的動情週期[(6.7±0.9)d]高于染氟60、120 d[(5.2±1.1)、(5.8±0.8)d,P均＜0.05].染氟60 d時,中、高氟組雌鼠E2含量[(438.75±71.80)、(456.25±57.80) ng/L]均高于對照組[(301.25±94.17)ng/L,P均＜0.05],隨染氟劑量增加而逐漸升高;僅高氟組在180 d E2水平[(275.00±127.83)ng/L]低于60、120 d[(456.25±57.80)、(392.50±102.50)ng/L,P均＜0.05].結論 燃煤型氟中毒可導緻雌鼠開張日齡和動情週期延長及生殖內分泌水平改變,且染氟劑量越多、染毒時間越長,影響越重.
목적 료해식용매불오염적양식대자서음도개장일령、동정주기급생식내분비공능적영향.방법 선용90지단유2주、체질량60～ 80g적SD자성대서,장대서안체질량채용수궤수자표법분위대조조、중불조、고불조,매조30지.각염불조통과위사함불동비례적연매형불중독병구매홍고옥미(함불량분별위47.8、96.0 mg/kg)복제연매형불중독모형,대조조적옥미사료래자우비병구(함불량위5.2 mg/kg).분별우염불60、120、180 d후,분3비동정기처사대서,매조매비10지.위양초기,매천조상8:00관찰병기록자서음도개장정황.처사전련속10 d,자서행음도탈락세포도편,HE염색후판별동정주기.우동정기고동맥취혈검측자격소(E2)수평.결과 고불조자서음도개장일령[(49.0±4.5)d]장우대조조、중불조[(45.4±2.3)、(46.7±1.9)d,P균＜0.05].염불120 d,고불조적자서동정주기[(5.8±0.8)d]장우대조조、중불조[(4.4±0.9)、(4.9±0.7)d,P균＜0.05].염불180 d,대조조、중불조、고불조적자서동정주기[(4.3±0.7)、(5.4±1.1)、(6.7±0.9)d]의차연장(P균＜ 0.05).재고불조,염불180 d자서적동정주기[(6.7±0.9)d]고우염불60、120 d[(5.2±1.1)、(5.8±0.8)d,P균＜0.05].염불60 d시,중、고불조자서E2함량[(438.75±71.80)、(456.25±57.80) ng/L]균고우대조조[(301.25±94.17)ng/L,P균＜0.05],수염불제량증가이축점승고;부고불조재180 d E2수평[(275.00±127.83)ng/L]저우60、120 d[(456.25±57.80)、(392.50±102.50)ng/L,P균＜0.05].결론 연매형불중독가도치자서개장일령화동정주기연장급생식내분비수평개변,차염불제량월다、염독시간월장,영향월중.
Objective To observe the impact of coal-burning-borne fluorosis on the opening day of vagina,and estrogen cycle and endocrinology of female rat.Methods Ninety weaned two weeks and healthy female SD rats,weighing 60-80 g were selected,and divided into three groups by body weight using random number table:control group,moderate fluoride exposed group,and higher fluoride exposed group,thirty rates in each group.Rats in fluoride exposed groups were fed with baked corn collected from coal-burning-borne fluorosis areas containing different levels of fluorine(fluorine contents were 47.8 and 96.0 mg/kg,respectively) to establish coal-burning-borne fluorosis models.The corn fed to control group rats were collected from non-endemic areas (fluoride content was 5.2 mg/kg).After exposed to fluoride for 60,120 and 180 days,the rats were put to death during estrus in three batches (10 rats in every batche).The situation of female vaginal opening was observed and recorded every morning at 8:00 at the beginning of the experiment.Ten female rats in each group were randomly selected to observe vaginal exfoliated cells for 10 days continuously every morning,and HE staining was selected to discriminate estrogen cycle.Femoral artery blood was collected to observe the impact of estradiol(E2) in estrus.Results The vaginal opening day age of the longer fluoride exposed group [(49.0 ± 4.5)d] was longer than that of the control group[(45.4 ± 2.3)d] and the moderate fluoride exposed group[(46.7 ± 1.9)d,all P ＜ 0.05].Estrogen cycle of the longer fluoride exposed group[(5.8 ± 0.8)d] after fluoride exposure for 120 d was higher than that of the control group [(4.4 + 0.9)d] and the moderate fluoride exposed group [(4.9 ± 0.7)d,all P ＜ 0.05].Estrogen cycle increased with increasing dosage of fluoride after fluoride exposure for 180 d in three groups [control group:(4.3 ± 0.7)d,moderate fluoride exposed group:(5.4 ± 1.1)d,higher fluoride exposed group:(6.7 + 0.9)d,all P ＜ 0.05].Estrogen cycle in higher fluoride exposed group at 180 d[(6.7 ± 0.9)d] was higher than that of 60 d and 120 d[(5.2 ± 1.1),(5.8 ± 0.8)d,all P ＜ 0.05].At 60 d after exposed to fluoride,E2 levels of fluoride exposed groups were increased[control group:(30.125 ± 94.17)ng/L,moderate fluoride exposed group:(438.75 ± 71.80) ng/L],higher fluoride exposed group (456.25 ± 57.80)ng/L with increasing dosage of fluoride (all P ＜ 0.05).E2 level in rats exposed to fluoride for 180 d [(275.00 ± 127.83)ng/L] was lower than that of 60 d and 120 d [(456.25 ± 57.80),(392.50 ± 102.50)ng/L,all P ＜ 0.05] in higher fluoride exposed groups.Conclusions Coal-burning-borne fluorosis has a significant effect on vaginal opening day age,estrogen cycle and endocrinology of female rats.The higher the fluoride dose,and the longer the exposure time,the more severe the impact.
