碘摄入过量对BALB/c小鼠白介素17、白介素23和干扰素-γ mRNA表达的影响
전섭입과량대BALB/c소서백개소17、백개소23화간우소-γ mRNA표체적영향
The effects of excessive iodine intake on mRNA expressions of interleukin-23, interleukin-17 and gamma interferon in BABL/c mice
  • 万方数据
    中华地方病学杂志 중화지방병학잡지
    Chinese Journal of Endemiology
    2014年 2期 145-149 ,共5页

    周晓丽%袁继红%郑楠%史亚男%胡志梅%刘欣%于秀杰%李兰英

    주효려%원계홍%정남%사아남%호지매%류흔%우수걸%리란영

    碘%甲状腺炎,自身免疫性%白介素17%白介素23%干扰素-γ 碘%甲狀腺炎,自身免疫性%白介素17%白介素23%榦擾素-γ
    전%갑상선염,자신면역성%백개소17%백개소23%간우소-γ
    Iodine%Thyroiditis,Autoimmune%Interleukin-17%Interleukin-23%Gamma interferon
    目的 观察碘摄入过量对小鼠自身免疫性甲状腺炎发病的影响以及白介素17(IL-17)、白介素23 (IL-23)、干扰素-γ(IFN-γ)在小鼠甲状腺、脾脏中mRNA表达的变化.方法 7～8周BALB/c小鼠48只,雌雄各半,体质量20～ 25 g,按体质量采用随机数字表法将小鼠分为4组(每组12只,雌雄各半).其中对照组饮去离子水;甲状腺球蛋白(TG)组饮去离子水,在小鼠8周龄时,给予0.1 mgTg皮下免疫,11周龄、15周龄时加强免疫2次;高碘(HI)组饮用0.05％碘化钠(NaI)水;甲状腺球蛋白+高碘(TG+ HI)组饮0.05％ NaI水,Tg免疫同TG组;4组动物均食用普通饲料.8周后处死小鼠,采用苏木精-伊红(HE)染色法观察甲状腺组织形态学变化;化学发光免疫分析法(CIA)检测小鼠血清中甲状腺激素;实时荧光定量PCR(RT-PCR)法检测小鼠甲状腺、脾脏中IL-17、IL-23、IFN-γmRNA的表达.结果 与对照组比较,IG组甲状腺滤泡较一致,HI、TG+ HI组甲状腺肿大,出现滤泡扩张、胶质潴留;IG组可见单个散在淋巴细胞,HI组少见淋巴细胞浸润,IG+HI组有局部淋巴细胞浸润、组织破坏.4组小鼠血清中游离三碘甲腺原氨酸(FT3)总甲状腺素(TT4)、游离甲状腺素(FT4)含量组间比较差异均有统计学意义(F值分别为12.54、4.00、10.62,P均＜0.05).4组小鼠甲状腺中IL-17、IL-23、IFN-γ mRNA表达组间比较差异均有统计学意义(F值分别为9.01、9.19、4.79,P均＜0.01),其中TG、TG+HI组IL-23 (0.257 6±0.169 3、0.262 0±0.126 3)、IFN-γ(0.185 0±0.171 0、0.144 8±0.078 4)mRNA表达均高于对照组(0.114 5±0.073 2、0.057 4±0.032 9,P均＜0.05),TG+ HI组IL-17 mRNA(0.017 4±0.017 8)表达高于对照组(0.000 6±0.000 3,P＜ 0.05).4组小鼠脾脏中IL-17、IL-23、IFN-γ mRNA表达组间比较差异均有统计学意义(F值分别为9.01、9.14、356,P均＜0.05),其中HI、TG+ HI组IL-23 (0.050 7±0.042 6、0.117 5±0.083 1)mRNA表达均高于对照组(0.004 8±0.005 2,P均＜0.05),TG、TG+ HI组IL-17 (0.007 8±0.006 6、0.009 6±0.010 0)、IFN-γ(0.219 3±0.061 1、0.222 8±0.208 0)mRNA表达均高于对照组(0.001 8±0.001 1、0.076 1±0.040 6,P均＜0.05).结论 碘摄入过量可导致小鼠发生甲状腺肿;Tg诱发小鼠发生自身免疫性甲状腺炎症后,碘过量可使IL-23、IL-17和IFN-γmRNA在小鼠甲状腺、脾脏中的表达量增加.
    목적 관찰전섭입과량대소서자신면역성갑상선염발병적영향이급백개소17(IL-17)、백개소23 (IL-23)、간우소-γ(IFN-γ)재소서갑상선、비장중mRNA표체적변화.방법 7～8주BALB/c소서48지,자웅각반,체질량20～ 25 g,안체질량채용수궤수자표법장소서분위4조(매조12지,자웅각반).기중대조조음거리자수;갑상선구단백(TG)조음거리자수,재소서8주령시,급여0.1 mgTg피하면역,11주령、15주령시가강면역2차;고전(HI)조음용0.05％전화납(NaI)수;갑상선구단백+고전(TG+ HI)조음0.05％ NaI수,Tg면역동TG조;4조동물균식용보통사료.8주후처사소서,채용소목정-이홍(HE)염색법관찰갑상선조직형태학변화;화학발광면역분석법(CIA)검측소서혈청중갑상선격소;실시형광정량PCR(RT-PCR)법검측소서갑상선、비장중IL-17、IL-23、IFN-γmRNA적표체.결과 여대조조비교,IG조갑상선려포교일치,HI、TG+ HI조갑상선종대,출현려포확장、효질저류;IG조가견단개산재림파세포,HI조소견림파세포침윤,IG+HI조유국부림파세포침윤、조직파배.4조소서혈청중유리삼전갑선원안산(FT3)총갑상선소(TT4)、유리갑상선소(FT4)함량조간비교차이균유통계학의의(F치분별위12.54、4.00、10.62,P균＜0.05).4조소서갑상선중IL-17、IL-23、IFN-γ mRNA표체조간비교차이균유통계학의의(F치분별위9.01、9.19、4.79,P균＜0.01),기중TG、TG+HI조IL-23 (0.257 6±0.169 3、0.262 0±0.126 3)、IFN-γ(0.185 0±0.171 0、0.144 8±0.078 4)mRNA표체균고우대조조(0.114 5±0.073 2、0.057 4±0.032 9,P균＜0.05),TG+ HI조IL-17 mRNA(0.017 4±0.017 8)표체고우대조조(0.000 6±0.000 3,P＜ 0.05).4조소서비장중IL-17、IL-23、IFN-γ mRNA표체조간비교차이균유통계학의의(F치분별위9.01、9.14、356,P균＜0.05),기중HI、TG+ HI조IL-23 (0.050 7±0.042 6、0.117 5±0.083 1)mRNA표체균고우대조조(0.004 8±0.005 2,P균＜0.05),TG、TG+ HI조IL-17 (0.007 8±0.006 6、0.009 6±0.010 0)、IFN-γ(0.219 3±0.061 1、0.222 8±0.208 0)mRNA표체균고우대조조(0.001 8±0.001 1、0.076 1±0.040 6,P균＜0.05).결론 전섭입과량가도치소서발생갑상선종;Tg유발소서발생자신면역성갑상선염증후,전과량가사IL-23、IL-17화IFN-γmRNA재소서갑상선、비장중적표체량증가.
    Objective To observe the effects of excessive iodine intake on autoimmune thyroiditis and mRNA expressions of interleukin-17 (IL-17),interleukin-23 (IL-23) and gamma interferon (IFN-γ) in thyroid gland and spleen of mice and to further explore the pathogenesis of iodine overdose induced autoimmune thyroiditis.Methods According to body mass,seven to eight weeks old BALB/c mice (body mass 20-25 g,half male and half female) were selected and divided into 4 groups (12 mice in each group,half male and half female) by random number table.Control group(CG) drank deionized water; thyroglobulin group(TG) drank deionized water,and were immunized with 0.1 mg thyroglobulin(Tg) subcutaneously at the age of 8 weeks and with two booster immunization at the age of 11 and 15 weeks,respectively; iodine excess group (HI) drank water containing 0.05％ sodium iodide (NaI); thyroglobulin + iodine excess(TG + HI) group drank water containing 0.05％ NaI,and were immunized the same way as TG group did.The 4 groups were fed with normal diet.After 8 weeks,hematoxylin-eosin (HE) staining was employed to observe the histopathology of thyroid; chemiluminescence immunoassay (CIA) was used to observe the level of thyroid hormone and real-time PCR (RT-PCR) was used to detect the level of inflammation related genes(IL-17,IL-23 and IFN-γ) in thyroid and spleen.Results Compared with CG group,goiter,follicular expansion and glial retention were observed in HI and TG + HI groups,but thyroid follicular was consistent in TG group.There was rare lymphocyte infiltration in HI group,but single scattered lymphocyte infiltration was detected in TG group,and local lymphocyte infiltration and destructed tissue were observed in TG + HI group.Among the 4 groups,the content of free triiodothyronine (FT3),total thyroxine (TT4) and free thyroxine (FT4) were significantly different between groups(F =12.54,4.00,10.62,all P ＜ 0.05).RT-PCR results showed that in thyroid,the relative expressions of IL-23,IL-17 and IFN-γ in the 4 groups were significantly different between groups(F =9.01,9.19,4.79,all P ＜ 0.01).The mRNA expressions of IL-23(0.257 6 + 0.169 3,0.262 0 + 0.126 3) and IFN-γ(0.185 0 + 0.170 1,0.144 8 ± 0.078 4) in TG and TG + HI groups were significantly increased compared with those of CG group (0.114 5 ± 0.073 2,0.057 4 + 0.032 9,all P ＜ 0.05) ; the expression of IL-17(0.017 4 ± 0.017 8) in TG + HI group was higher than that of CG group(0.000 6 ± 0.00 3,P＜ 0.05).In spleen,the expressions of IL-23,IL-17 and IFN-γ in the 4 groups were significantly different between groups (F =9.01,9.14,3.56,all P ＜ 0.05).In TG and TG + HI groups,the mRNA expressions of IL-17(0.007 8 ± 0.006 6,0.009 6 + 0.010 0) and IFN-γ(0.219 3 + 0.061 1,0.222 8 + 0.208 0) were higher than those of CG group(0.001 8 ± 0.001 1,0.076 1 ± 0.046 0,all P ＜ 0.05); the mRNA expressions of IL-23(0.050 7 ± 0.042 6,0.117 5 ± 0.083 1) in HI and TG + HI groups were higher than that of CG group(0.004 8 ± 0.005 2,all P ＜ 0.05).Conclusions Excessive iodine intake can lead to mice goiter; in TG-induced autoimmune thyroid inflammation,iodine excess can lead to increased mRNA expressions of IL-23,IL-17 and IFN-γ in thyroid gland and spleen of mice.
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