中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2014年
2期
150-154
,共5页
于慧敏%王茜%夏荣香%魏洁群%吴军%郑玉建
于慧敏%王茜%夏榮香%魏潔群%吳軍%鄭玉建
우혜민%왕천%하영향%위길군%오군%정옥건
亚砷酸钠%砷酸钠%大鼠%肾%代谢
亞砷痠鈉%砷痠鈉%大鼠%腎%代謝
아신산납%신산납%대서%신%대사
Sodium arsenite%Sodium dihydrogen arsenate%Rats%Kidney%Metabolism
目的 探讨不同价态砷染毒雄性大鼠肾脏砷代谢产物与相关代谢酶之间的关系.方法 Wistar 雄性大鼠35只,体质量150 ～ 190 g,按体质量采用随机数字表法分为7组,每组5只大鼠.其中亚砷酸钠低、中、高剂量组染砷(亚砷酸钠)剂量分别为2.2、6.7、20.0 mg/kg;砷酸钠低、中、高剂量组染砷(砷酸钠)剂量分别为2.2、6.7、20.0 mg/kg;对照组饮用去离子水.染砷3个月后,处死动物,取大鼠肾脏,-80℃冷冻保存.采用高效液相色谱-氢化物发生原子荧光光谱法(HPLC-HGAFS)检测大鼠肾脏中各形态砷代谢产物,酶联免疫法检测并分析相关代谢酶的含量、活力,并探讨砷代谢产物与相关代谢酶之间的关系.结果 各组大鼠肾脏总砷(TAs)、二甲基砷(DMA)、一甲基砷(MMA)、甲基转移酶活力组间比较差异有统计学意义(F值为1874.672,H值分别为33513、31.002,F值为79.607,P均＜0.01).其中亚砷酸钠低、中、高剂量组TAs[(526.52±25.56)、(1 654.00±101.55)、(1 904.24±104.76) μg/kg]和DMA[(323.20±16.13)、(1 444.40±113.81)、(1 765.40±104.39)μg/kg]均高于砷酸钠相应各剂量组[(235.70±6.23)、(471.05±18.32)、(1 677.40±83.29) μg/kg,(0.00±0.00)、(1.75±0.16)、(410.50±19.76)μg/kg,P＜0.0024或＜0.05];亚砷酸钠低、中、高剂量组MMA[(4.02±0.86)、(4.20±0.65)、(4.04±0.80)μg/kg]均低于砷酸钠相应各剂量组[(98.90±9.59)、(376.50±15.41)、(1 131.90±74.26)μg/kg,P均＜0.05];亚砷酸钠低、中、高剂量组甲基转移酶活力[(7.80±0.93)、(5.55±0.49)、(3.56±0.26)U/g]均低于砷酸钠相应各剂量组[(11.59±0.93)、(8.93±0.88)、(6.52±1.04)U/g,P均＜0.0024].亚砷酸钠低、中、高剂量组DMA和砷酸钠中、高剂量组MMA与相应各组TAs均呈正相关[相关系数(r)值分别为0.970、0.984、0.997、0.947、0.961,P均＜0.05].结论 不同价态砷大鼠肾脏砷代谢产物与相关代谢酶的作用不同,砷酸钠在肾脏的代谢产物对甲基转移酶活力的促进作用强于亚砷酸钠,影响砷甲基化代谢产物在肾脏部位的数量及分布.
目的 探討不同價態砷染毒雄性大鼠腎髒砷代謝產物與相關代謝酶之間的關繫.方法 Wistar 雄性大鼠35隻,體質量150 ～ 190 g,按體質量採用隨機數字錶法分為7組,每組5隻大鼠.其中亞砷痠鈉低、中、高劑量組染砷(亞砷痠鈉)劑量分彆為2.2、6.7、20.0 mg/kg;砷痠鈉低、中、高劑量組染砷(砷痠鈉)劑量分彆為2.2、6.7、20.0 mg/kg;對照組飲用去離子水.染砷3箇月後,處死動物,取大鼠腎髒,-80℃冷凍保存.採用高效液相色譜-氫化物髮生原子熒光光譜法(HPLC-HGAFS)檢測大鼠腎髒中各形態砷代謝產物,酶聯免疫法檢測併分析相關代謝酶的含量、活力,併探討砷代謝產物與相關代謝酶之間的關繫.結果 各組大鼠腎髒總砷(TAs)、二甲基砷(DMA)、一甲基砷(MMA)、甲基轉移酶活力組間比較差異有統計學意義(F值為1874.672,H值分彆為33513、31.002,F值為79.607,P均＜0.01).其中亞砷痠鈉低、中、高劑量組TAs[(526.52±25.56)、(1 654.00±101.55)、(1 904.24±104.76) μg/kg]和DMA[(323.20±16.13)、(1 444.40±113.81)、(1 765.40±104.39)μg/kg]均高于砷痠鈉相應各劑量組[(235.70±6.23)、(471.05±18.32)、(1 677.40±83.29) μg/kg,(0.00±0.00)、(1.75±0.16)、(410.50±19.76)μg/kg,P＜0.0024或＜0.05];亞砷痠鈉低、中、高劑量組MMA[(4.02±0.86)、(4.20±0.65)、(4.04±0.80)μg/kg]均低于砷痠鈉相應各劑量組[(98.90±9.59)、(376.50±15.41)、(1 131.90±74.26)μg/kg,P均＜0.05];亞砷痠鈉低、中、高劑量組甲基轉移酶活力[(7.80±0.93)、(5.55±0.49)、(3.56±0.26)U/g]均低于砷痠鈉相應各劑量組[(11.59±0.93)、(8.93±0.88)、(6.52±1.04)U/g,P均＜0.0024].亞砷痠鈉低、中、高劑量組DMA和砷痠鈉中、高劑量組MMA與相應各組TAs均呈正相關[相關繫數(r)值分彆為0.970、0.984、0.997、0.947、0.961,P均＜0.05].結論 不同價態砷大鼠腎髒砷代謝產物與相關代謝酶的作用不同,砷痠鈉在腎髒的代謝產物對甲基轉移酶活力的促進作用彊于亞砷痠鈉,影響砷甲基化代謝產物在腎髒部位的數量及分佈.
목적 탐토불동개태신염독웅성대서신장신대사산물여상관대사매지간적관계.방법 Wistar 웅성대서35지,체질량150 ～ 190 g,안체질량채용수궤수자표법분위7조,매조5지대서.기중아신산납저、중、고제량조염신(아신산납)제량분별위2.2、6.7、20.0 mg/kg;신산납저、중、고제량조염신(신산납)제량분별위2.2、6.7、20.0 mg/kg;대조조음용거리자수.염신3개월후,처사동물,취대서신장,-80℃냉동보존.채용고효액상색보-경화물발생원자형광광보법(HPLC-HGAFS)검측대서신장중각형태신대사산물,매련면역법검측병분석상관대사매적함량、활력,병탐토신대사산물여상관대사매지간적관계.결과 각조대서신장총신(TAs)、이갑기신(DMA)、일갑기신(MMA)、갑기전이매활력조간비교차이유통계학의의(F치위1874.672,H치분별위33513、31.002,F치위79.607,P균＜0.01).기중아신산납저、중、고제량조TAs[(526.52±25.56)、(1 654.00±101.55)、(1 904.24±104.76) μg/kg]화DMA[(323.20±16.13)、(1 444.40±113.81)、(1 765.40±104.39)μg/kg]균고우신산납상응각제량조[(235.70±6.23)、(471.05±18.32)、(1 677.40±83.29) μg/kg,(0.00±0.00)、(1.75±0.16)、(410.50±19.76)μg/kg,P＜0.0024혹＜0.05];아신산납저、중、고제량조MMA[(4.02±0.86)、(4.20±0.65)、(4.04±0.80)μg/kg]균저우신산납상응각제량조[(98.90±9.59)、(376.50±15.41)、(1 131.90±74.26)μg/kg,P균＜0.05];아신산납저、중、고제량조갑기전이매활력[(7.80±0.93)、(5.55±0.49)、(3.56±0.26)U/g]균저우신산납상응각제량조[(11.59±0.93)、(8.93±0.88)、(6.52±1.04)U/g,P균＜0.0024].아신산납저、중、고제량조DMA화신산납중、고제량조MMA여상응각조TAs균정정상관[상관계수(r)치분별위0.970、0.984、0.997、0.947、0.961,P균＜0.05].결론 불동개태신대서신장신대사산물여상관대사매적작용불동,신산납재신장적대사산물대갑기전이매활력적촉진작용강우아신산납,영향신갑기화대사산물재신장부위적수량급분포.
Objective To investigate the relationship between metabolites of sodium arsenite and sodium dihydrogen arsenate with related metabolic enzymes in kidney of male rats.Methods According to body mass,thirty-five male Wistar rats(body mass 150-190 g) were divided into 7 groups by random number table.Control group drank deionized water; the contents of iAsⅢ in low,medium and high arsenite groups and the contents of iAsv in low,medium and high of sodium dihydrogen arsenate groups were 2.2,6.7 and 20.0 mg/kg,respectively.After 3 months,kidneys were collected and stored at-80 C; high performance liquid chromatography and hydride genesis atomic fluorescence spectroscopy (HPLC-HGAFS) was used to determine the level of arsenic metabolites in kidney,and enzyme-linked immunosorbent assay was used to detect and analyze the content or the activity of metabolic enzymes,meanwhile correlation studies between the level of metabolites and the activity of metabolic enzymes were carried out.Results The differences of total arsenic (TAs),dimethyl arsenic acid (DMA),monomethyl arsenic acid (MMA) and methyl transferase enzyme activity in kidneys of rats between groups were statistically significant (F =1874.672,H =33.513,31.002,F =79.607,all P ＜ 0.01).The TAs[(526.52 ± 25.56),(1 654.00 ± 101.55),(1 904.24 ± 104.76)μg,/kg] and DMA[(323.20 + 16.13),(1 444.40 ± 113.81),(1 765.40 ± 104.39)μg/kg] of sodium arsenite in low,medium and high dose groups were higher than those of the corresponding sodium dihydrogen arsenate groups [(235.70 ± 6.23),(471.05 ± 18.32),(1 677.40 ± 83.29)μg/kg,and(0.00 ± 0.00),(1.75 ± 0.16),(410.50 ± 19.76)μg/kg,P ＜ 0.0024 or ＜ 0.05] ; the MMA[(4.02 + 0.86),(4.20 ± 0.65),(4.04 ± 0.80)μg/kg] of sodium arsenite in low,medium and high dose groups were lower than those of the corresponding sodium dihydrogen arsenate groups[(98.90 ± 9.59),(376.50 ± 15.41),(1 131.90 ± 74.26) μg/kg,all P＜ 0.05]; the methyl transferase enzyme activities[(7.80 ± 0.93),(5.55 ± 0.49),(3.56 ± 0.26)U/g] of sodium arsenite in low,medium and high dose groups were lower than those of the corresponding sodium dihydrogen arsenate group[(11.59 ± 0.93),(8.93 ± 0.88),(6.52 ± 1.04)U/g,all P ＜ 0.0024].The DMA of sodium arsenite in low,medium and high dose groups,the MMA of sodium dihydrogen arsenate in medium and high dose groups were positively correlated with those of TAs in each group(r =0.970,0.984,0.997,0.947,0.961,all P ＜ 0.05).Conclusions Effects of sodium arsenite and sodium dihydrogen arsenate on arsenic metobdites and related metabolic enzymes in kidney of rats are different.The function of sodium dihydrogen arsenate in promoting methyl transferase activity is stronger than that of sodium arsenite,which affects the amount and distribution of arsenic methylation metabolites in kidney.
