中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2012年
22期
3361-3364,后插1
,共5页
付兴华%张萍%邓艳丽%卢鹤翔%孙瑞军%刘颖%赵君晖%顾洪兰%韩莉%张再兴
付興華%張萍%鄧豔麗%盧鶴翔%孫瑞軍%劉穎%趙君暉%顧洪蘭%韓莉%張再興
부흥화%장평%산염려%로학상%손서군%류영%조군휘%고홍란%한리%장재흥
弥漫性脑外伤%锌原卟啉%血红素%耳蜗%血红素加氧酶%听性脑干反应
瀰漫性腦外傷%鋅原卟啉%血紅素%耳蝸%血紅素加氧酶%聽性腦榦反應
미만성뇌외상%자원계람%혈홍소%이와%혈홍소가양매%은성뇌간반응
Diffuse traumatic brain injury%Zinc Protoporphyrin%Heme%Cochlear%Heme oxygenase%Auditory Brainstem Response
目的 探讨锌原卟啉(Znpp)与血红素(Heme)对弥漫性脑外伤大鼠耳蜗血红素加氧酶(HO-1)表达及听性脑干反应(ABR)的影响.方法 建立弥漫性脑外伤大鼠模型并随机分组,采用ABR测定、光镜、免疫组织化学等方法,观察各组动物耳蜗HO-1表达及ABR的变化.结果 外伤后各组ABR阈值及各波潜伏期差异均有统计学意义(均P＜0.05).对照组大鼠耳蜗HO-1无表达,外伤后各组耳蜗HO-1表达有明显变化(P＜0.01).正常对照组与DBI组、Znpp、Heme各组灰度值差异均有统计学意义(均P＜0.05).结论 锌原卟啉与血红素对弥漫性脑外伤大鼠耳蜗HO-1表达及ABR均有不同程度的影响,血红素对听功能的保护作用可能与HO-1表达升高有关.
目的 探討鋅原卟啉(Znpp)與血紅素(Heme)對瀰漫性腦外傷大鼠耳蝸血紅素加氧酶(HO-1)錶達及聽性腦榦反應(ABR)的影響.方法 建立瀰漫性腦外傷大鼠模型併隨機分組,採用ABR測定、光鏡、免疫組織化學等方法,觀察各組動物耳蝸HO-1錶達及ABR的變化.結果 外傷後各組ABR閾值及各波潛伏期差異均有統計學意義(均P＜0.05).對照組大鼠耳蝸HO-1無錶達,外傷後各組耳蝸HO-1錶達有明顯變化(P＜0.01).正常對照組與DBI組、Znpp、Heme各組灰度值差異均有統計學意義(均P＜0.05).結論 鋅原卟啉與血紅素對瀰漫性腦外傷大鼠耳蝸HO-1錶達及ABR均有不同程度的影響,血紅素對聽功能的保護作用可能與HO-1錶達升高有關.
목적 탐토자원계람(Znpp)여혈홍소(Heme)대미만성뇌외상대서이와혈홍소가양매(HO-1)표체급은성뇌간반응(ABR)적영향.방법 건립미만성뇌외상대서모형병수궤분조,채용ABR측정、광경、면역조직화학등방법,관찰각조동물이와HO-1표체급ABR적변화.결과 외상후각조ABR역치급각파잠복기차이균유통계학의의(균P＜0.05).대조조대서이와HO-1무표체,외상후각조이와HO-1표체유명현변화(P＜0.01).정상대조조여DBI조、Znpp、Heme각조회도치차이균유통계학의의(균P＜0.05).결론 자원계람여혈홍소대미만성뇌외상대서이와HO-1표체급ABR균유불동정도적영향,혈홍소대은공능적보호작용가능여HO-1표체승고유관.
Objective To explore the effects of Zinc Protoporphyrin and Heme on the expression of HO-1 in cochlear and the change of auditory brainstem response on diffuse traumatic brain injury model of rats.Methods Diffuse traumatic brain injury model of rats were established and randomly divided into thirteen groups.Then auditory brainstem response examination,light microscope,immunohistochemistry technique were used to evaluate the change of auditory brainstem response and the expression of HO-1 in cochlear.Results The differences of auditory brainstem response threshold and latency of wave between the experimental and the normal control group were obvious(P＜0.05).The expression of HO-1 in the control group was normal,whereas there were obvious changes of inner ear HO-1 expression in the traumatic groups.The grey value of HO-1 expression in trauma group,Znpp group and heme group was significantly associated with auditory function change(P＜0.05).Conclusion There were influence of Zinc Protoporphyrin and Hemeon the inner ear HO-1 expression and the change of auditory brainstem response with diffuse traumatic brain injury model of rats.The protective effect of heme on auditory function may be associated with the increased expression of HO-1.
