中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2013年
11期
1601-1603,后插1
,共4页
张杰%王雪丁%陈卓佳%周珏倩%李嘉丽%毕惠嫦%钟国平%周列民%黄民
張傑%王雪丁%陳卓佳%週玨倩%李嘉麗%畢惠嫦%鐘國平%週列民%黃民
장걸%왕설정%진탁가%주각천%리가려%필혜항%종국평%주렬민%황민
拉莫三嗪%高效液相色谱法%药物监测%浓度剂量相关性
拉莫三嗪%高效液相色譜法%藥物鑑測%濃度劑量相關性
랍막삼진%고효액상색보법%약물감측%농도제량상관성
Lamotrigine%HPLC%TDM%Concentration-dose correlation
目的 建立快捷、灵敏、简便的高效液相色谱法(HPLC)测定拉莫三嗪(LTG)浓度,并应用于治疗药物浓度监测和药物相互作用研究.方法 以非那西丁为内标,检测波长为240 nm,使用Xterra MS C18色谱柱(4.6 mm×150 mm,5μm)为分析柱,以醋酸乙酯 二氯甲烷-叔丁基甲醚(3:1:1)为萃取剂,流动相为甲醇:水(含20 mM醋酸铵盐)(35:65,V/V),流速为1 ml/min.结果 LTG线性范围在0.1 ～ 50 μg/ml范围内线性良好(r ＞0.999),提取回收率为77.42％～83.05％,批间批内RSD均小于10％.应用HPLC检测使用LTG的87例癫痫患者血浆样本,对日剂量和血药浓度建立简单线性回归方程进行分析,血药浓度个体间差异大,剂量决定力不强(R2 =0.340).结论 HPLC简便、灵敏,可用于临床药物监测和研究,以辅助LTG个体化治疗.
目的 建立快捷、靈敏、簡便的高效液相色譜法(HPLC)測定拉莫三嗪(LTG)濃度,併應用于治療藥物濃度鑑測和藥物相互作用研究.方法 以非那西丁為內標,檢測波長為240 nm,使用Xterra MS C18色譜柱(4.6 mm×150 mm,5μm)為分析柱,以醋痠乙酯 二氯甲烷-叔丁基甲醚(3:1:1)為萃取劑,流動相為甲醇:水(含20 mM醋痠銨鹽)(35:65,V/V),流速為1 ml/min.結果 LTG線性範圍在0.1 ～ 50 μg/ml範圍內線性良好(r ＞0.999),提取迴收率為77.42％～83.05％,批間批內RSD均小于10％.應用HPLC檢測使用LTG的87例癲癇患者血漿樣本,對日劑量和血藥濃度建立簡單線性迴歸方程進行分析,血藥濃度箇體間差異大,劑量決定力不彊(R2 =0.340).結論 HPLC簡便、靈敏,可用于臨床藥物鑑測和研究,以輔助LTG箇體化治療.
목적 건립쾌첩、령민、간편적고효액상색보법(HPLC)측정랍막삼진(LTG)농도,병응용우치료약물농도감측화약물상호작용연구.방법 이비나서정위내표,검측파장위240 nm,사용Xterra MS C18색보주(4.6 mm×150 mm,5μm)위분석주,이작산을지 이록갑완-숙정기갑미(3:1:1)위췌취제,류동상위갑순:수(함20 mM작산안염)(35:65,V/V),류속위1 ml/min.결과 LTG선성범위재0.1 ～ 50 μg/ml범위내선성량호(r ＞0.999),제취회수솔위77.42％～83.05％,비간비내RSD균소우10％.응용HPLC검측사용LTG적87례전간환자혈장양본,대일제량화혈약농도건립간단선성회귀방정진행분석,혈약농도개체간차이대,제량결정력불강(R2 =0.340).결론 HPLC간편、령민,가용우림상약물감측화연구,이보조LTG개체화치료.
Objective To develop a simple,quick and fully validated HPLC method for determination of lamotrigine (LTG) in human plasma for therapy drug monitor.Methods Phenacetin was used as internal standard (IS).A simple liquid-liquid extraction process was employed to extract LTG and IS from human plasma which had been basified before.Chromatographic separation was performed on a Xterra MS C18 column (4.6mm × 150mm,3 μm),and the wave length of detection was 240nm.The mobile phase consisted of methanol-water(containing 20 mM ammonium acetate) (35:65,V/V).Results A linear response function was established for the range of concentrations 0.1 ～ 50μg/ml(r ＞ 0.999) for LTG.The recoveries of this method were 77.42％ ～ 83.05％ for LTG.The intraand inter-day precision values (RSD) was less than 10％.A total of 87 plasma samples of epilepsy patients had been assayed via HPLC.A linear regression was employed to explore the correlation between the dose and concentration in these patients,indicating low determination of dose factor(R2 =0.340)to concentration and wide individual variation.Conclusion HPLC had been proved to be quick,convenient,suitable for the study and therapy drug monitor to individualise patient therapy of LTG.
