中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2013年
19期
2890-2892
,共3页
热休克蛋白%休克,出血性%肠黏膜%血气分析%鼠科
熱休剋蛋白%休剋,齣血性%腸黏膜%血氣分析%鼠科
열휴극단백%휴극,출혈성%장점막%혈기분석%서과
Heat-shock proteins%Shock,hemorrhagic%Intestinal mucosa%Blood gas analysis%Muridae
目的 研究失血性休克大鼠股动脉血气分析和平均动脉压(MAP)的变化情况,进一步探索血红素加氧酶-1(HO-1)的保护机制.方法 将20只健康、清洁级、雄性SD大鼠复制失血性休克模型,随机分为:HO-1重组乳酸乳球菌灌胃组(HO组)和磷酸盐缓冲液(PBS)灌胃组(PBS组).比较两组血气分析、MAP,液体复苏后1h死亡率、细菌移位、髓过氧化物酶(MPO)活性及HO-1、肿瘤坏死因子-α(TNF-α)和白细胞介素-10(IL-10)的含量,并行肠组织病理学检查.结果 与PBS组比较,HO组的死亡率、Chiu's评分、细菌移位发生率、MPO活性明显降低[10％与40％、(1.51±0.23)分与(2.15±0.48)分、44.4％与100.0％、(0.16±0.05)U/g与(0.99±0.28) U/g,均P＜0.05]；复苏后的PaO2、MAP、HO-1含量和IL-10灰度值明显增加(均P ＜0.05).结论 该实验条件下,HO-1重组乳酸乳球菌在失血性休克期能够维持较高的PaO2和MAP,利于肠黏膜屏障保护和减轻肠道炎性反应.
目的 研究失血性休剋大鼠股動脈血氣分析和平均動脈壓(MAP)的變化情況,進一步探索血紅素加氧酶-1(HO-1)的保護機製.方法 將20隻健康、清潔級、雄性SD大鼠複製失血性休剋模型,隨機分為:HO-1重組乳痠乳毬菌灌胃組(HO組)和燐痠鹽緩遲液(PBS)灌胃組(PBS組).比較兩組血氣分析、MAP,液體複囌後1h死亡率、細菌移位、髓過氧化物酶(MPO)活性及HO-1、腫瘤壞死因子-α(TNF-α)和白細胞介素-10(IL-10)的含量,併行腸組織病理學檢查.結果 與PBS組比較,HO組的死亡率、Chiu's評分、細菌移位髮生率、MPO活性明顯降低[10％與40％、(1.51±0.23)分與(2.15±0.48)分、44.4％與100.0％、(0.16±0.05)U/g與(0.99±0.28) U/g,均P＜0.05]；複囌後的PaO2、MAP、HO-1含量和IL-10灰度值明顯增加(均P ＜0.05).結論 該實驗條件下,HO-1重組乳痠乳毬菌在失血性休剋期能夠維持較高的PaO2和MAP,利于腸黏膜屏障保護和減輕腸道炎性反應.
목적 연구실혈성휴극대서고동맥혈기분석화평균동맥압(MAP)적변화정황,진일보탐색혈홍소가양매-1(HO-1)적보호궤제.방법 장20지건강、청길급、웅성SD대서복제실혈성휴극모형,수궤분위:HO-1중조유산유구균관위조(HO조)화린산염완충액(PBS)관위조(PBS조).비교량조혈기분석、MAP,액체복소후1h사망솔、세균이위、수과양화물매(MPO)활성급HO-1、종류배사인자-α(TNF-α)화백세포개소-10(IL-10)적함량,병행장조직병이학검사.결과 여PBS조비교,HO조적사망솔、Chiu's평분、세균이위발생솔、MPO활성명현강저[10％여40％、(1.51±0.23)분여(2.15±0.48)분、44.4％여100.0％、(0.16±0.05)U/g여(0.99±0.28) U/g,균P＜0.05]；복소후적PaO2、MAP、HO-1함량화IL-10회도치명현증가(균P ＜0.05).결론 해실험조건하,HO-1중조유산유구균재실혈성휴극기능구유지교고적PaO2화MAP,리우장점막병장보호화감경장도염성반응.
Objective To study the protection mechanism of HO-1 with the gas-blood analysis and MAP of femoral artery in rats with hemorrhagic shock.Methods A model of hemorrhagic shock was established in 20 SD healthy clean male rats.The rats were randomly divided into the L.Lactis recombinant HO-1 gene group (HO group)and phosphate buffer solution group (PBS group).The gas-blood analysis and MAP of femoral artery in hemorrhagic shock were compared during the test.The mortality rate,MPO activity,bacterial translocation,the pathologic and content of HO-1,TNF-α and IL-10 in the low intestine were detected and compared 1h after resuscitation.Results Compared to PBS group,the mortality rate,Chiu's grade,bacterial translocation and MPO activity in HO group were significantly decreased[10％ vs40％,(1.51 ±0.23) points vs(2.15 ±0.48) points,44.4％ vs 100.0％,(0.16 ±0.05)U/g vs (0.99 ± 0.28)U/g,all P ＜0.05],PaO2 and MAP during the resuscitation,the content of HO-1 and the gray level of IL-10 were significantly increased.Conclusion L Lactis recombinant HO-1 gene has the virtue to maintain the higher level of PaO2 and MAP,which is beneficial to the intestine mucosa barrier and anti-inflammation response of low intestine significantly.
