中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2014年
6期
818-819
,共2页
羰基镍%血清镍
羰基鎳%血清鎳
탄기얼%혈청얼
Carbonyl nickel%Serum nickel
目的 探讨急性羰基镍染毒大鼠血清镍水平的变化规律,为临床救治急性羰基镍中毒患者提供实验室支持依据.方法 以羰基镍250 mg./in3、500 mg/m3两个剂量组给予SPF级大鼠各54只静态吸入30 min,分别于染毒后30 min、2h、4h、8h、12h、24 h、48 h、72 h、7d将大鼠以乙醚在15 s内麻醉,立即剖腹暴露腹主动脉,采集2 ～3 mL血液,分离0.5 ～1 mL血清,用美国PE公司AA800原子吸收仪(石墨炉法)检测血清镍含量,并与健康大鼠相比较.结果 250 mg/m3剂量组30 min、2h、4h、8 h、12 h、24h、48 h、72 h、7d血清镍含量平均值分别为(33.69±2.59) μg/L、(24.61 ±3.03) μg/L、(27.83±5.69)μg/L、(21.36±4.14)μg/L、(20.39 ±4.14) μg/L、(18.80±7.02)μg/L、(14.51±8.21) μg/L、(13.58±5.78)μg/L、(12.83±4.41)μg/L,30 min为峰值,为对照组的5.30倍,各时间段与健康大鼠差异均有统计学意义(t值分别为5.959、5.958、5.990、5.998、5.997、5.994、5.990、4.317、4.347,均P＜0.01).500 mg/m3剂量组30 min、2h、4h、8h、12 h、24h、48 h、72 h、7d血清镍含量平均值分别为(72.22±1.62) μg/L、(57.78±12.99) μg/L、(42.25±7.25)μg/L、(103.77±11.ll)μg/L、(79.04±12.26) μg/L、(26.35±6.56)μg/L、(18.58±4.92)μg/L、(17.22±9.73) μg/L、(14.59±5.27) μg/L,8h为峰值,为健康大鼠的16.33倍,各时间段与健康大鼠差异均有统计学意义(t值分别为5.960、5.947、5.978、5.927、5.948、5.959、3.143、2.447、2.440,均P＜0.05).两组30 min、2h、4h、8h、12h等时间段差异均有统计学意义(t值分别为5.208、2.447、2.449、5.959、5.959,P值分别为0.001、0.049、0.042、0.000、0.000).结论 急性羰基镍染毒大鼠血清镍含量与染毒剂量呈明显的剂量-效应关系;羰基镍或其代谢产物的排出主要在24h内.
目的 探討急性羰基鎳染毒大鼠血清鎳水平的變化規律,為臨床救治急性羰基鎳中毒患者提供實驗室支持依據.方法 以羰基鎳250 mg./in3、500 mg/m3兩箇劑量組給予SPF級大鼠各54隻靜態吸入30 min,分彆于染毒後30 min、2h、4h、8h、12h、24 h、48 h、72 h、7d將大鼠以乙醚在15 s內痳醉,立即剖腹暴露腹主動脈,採集2 ～3 mL血液,分離0.5 ～1 mL血清,用美國PE公司AA800原子吸收儀(石墨爐法)檢測血清鎳含量,併與健康大鼠相比較.結果 250 mg/m3劑量組30 min、2h、4h、8 h、12 h、24h、48 h、72 h、7d血清鎳含量平均值分彆為(33.69±2.59) μg/L、(24.61 ±3.03) μg/L、(27.83±5.69)μg/L、(21.36±4.14)μg/L、(20.39 ±4.14) μg/L、(18.80±7.02)μg/L、(14.51±8.21) μg/L、(13.58±5.78)μg/L、(12.83±4.41)μg/L,30 min為峰值,為對照組的5.30倍,各時間段與健康大鼠差異均有統計學意義(t值分彆為5.959、5.958、5.990、5.998、5.997、5.994、5.990、4.317、4.347,均P＜0.01).500 mg/m3劑量組30 min、2h、4h、8h、12 h、24h、48 h、72 h、7d血清鎳含量平均值分彆為(72.22±1.62) μg/L、(57.78±12.99) μg/L、(42.25±7.25)μg/L、(103.77±11.ll)μg/L、(79.04±12.26) μg/L、(26.35±6.56)μg/L、(18.58±4.92)μg/L、(17.22±9.73) μg/L、(14.59±5.27) μg/L,8h為峰值,為健康大鼠的16.33倍,各時間段與健康大鼠差異均有統計學意義(t值分彆為5.960、5.947、5.978、5.927、5.948、5.959、3.143、2.447、2.440,均P＜0.05).兩組30 min、2h、4h、8h、12h等時間段差異均有統計學意義(t值分彆為5.208、2.447、2.449、5.959、5.959,P值分彆為0.001、0.049、0.042、0.000、0.000).結論 急性羰基鎳染毒大鼠血清鎳含量與染毒劑量呈明顯的劑量-效應關繫;羰基鎳或其代謝產物的排齣主要在24h內.
목적 탐토급성탄기얼염독대서혈청얼수평적변화규률,위림상구치급성탄기얼중독환자제공실험실지지의거.방법 이탄기얼250 mg./in3、500 mg/m3량개제량조급여SPF급대서각54지정태흡입30 min,분별우염독후30 min、2h、4h、8h、12h、24 h、48 h、72 h、7d장대서이을미재15 s내마취,립즉부복폭로복주동맥,채집2 ～3 mL혈액,분리0.5 ～1 mL혈청,용미국PE공사AA800원자흡수의(석묵로법)검측혈청얼함량,병여건강대서상비교.결과 250 mg/m3제량조30 min、2h、4h、8 h、12 h、24h、48 h、72 h、7d혈청얼함량평균치분별위(33.69±2.59) μg/L、(24.61 ±3.03) μg/L、(27.83±5.69)μg/L、(21.36±4.14)μg/L、(20.39 ±4.14) μg/L、(18.80±7.02)μg/L、(14.51±8.21) μg/L、(13.58±5.78)μg/L、(12.83±4.41)μg/L,30 min위봉치,위대조조적5.30배,각시간단여건강대서차이균유통계학의의(t치분별위5.959、5.958、5.990、5.998、5.997、5.994、5.990、4.317、4.347,균P＜0.01).500 mg/m3제량조30 min、2h、4h、8h、12 h、24h、48 h、72 h、7d혈청얼함량평균치분별위(72.22±1.62) μg/L、(57.78±12.99) μg/L、(42.25±7.25)μg/L、(103.77±11.ll)μg/L、(79.04±12.26) μg/L、(26.35±6.56)μg/L、(18.58±4.92)μg/L、(17.22±9.73) μg/L、(14.59±5.27) μg/L,8h위봉치,위건강대서적16.33배,각시간단여건강대서차이균유통계학의의(t치분별위5.960、5.947、5.978、5.927、5.948、5.959、3.143、2.447、2.440,균P＜0.05).량조30 min、2h、4h、8h、12h등시간단차이균유통계학의의(t치분별위5.208、2.447、2.449、5.959、5.959,P치분별위0.001、0.049、0.042、0.000、0.000).결론 급성탄기얼염독대서혈청얼함량여염독제량정명현적제량-효응관계;탄기얼혹기대사산물적배출주요재24h내.
Objective To master the serum nickel change rule of acute nickel carbonyl poisoning in rats,and to provide laboratory support for clinical treatment of acute nickel carbonyl poisoning patients.Methods SPF rats were given nickel carbonyl (250mg/m3,500mg/m3) in static inhalation for 30min.Rats were anesthetized by ether for 15 seconds after exposure for 30min,2h,4h,8h,12h,24h,48h,72h and 7d respectively.Anatomized rats,kept blood collection 2-3mL,and separated serum 0.5-1mL.Serum nickel was detected by AA800 (American PE company).Results The average serum nickel levels of 250mg/m3 dose group at 30min,2h,4h,8h,12h,24h,48h,72h,7d were (33.69 ±2.59),(24.61 ±3.03),(27.83 ±5.69),(21.36 ±4.14),(20.39 ±4.14),(18.80 ±7.02),(14.51 ±8.21),(13.58 ± 5.78) and (12.83 ± 4.41)μg/L.30 minutes reached the peak,and was 5.30-fold of those in normal controls.There had significant differences compared with normal control(t =5.959,5.958,5.990,5.998,5.997,5.994,5.990,4.317,4.347,all P ＜ 0.01).The average serum nickel levels of 500mg/m3 dose group at 30min,2h,4h,8h,12h,24h,48h,72h,7d were (72.22 ± 1.62),(57.78 ± 12.99),(42.25 ± 7.25),(103.77 ± 11.11),(79.04 ±12.26),(26.35 ±6.56),(18.58 ±4.92),(17.22 ±9.73),(14.59 ±5.27) μg/L.8h reached the peak,and was 16.33-fold of normal controls.The differences were significant (t =5.960,5.947,5.978,5.927,5.948,5.959,3.143,2.447,2.440,all P ＜ 0.05).Meanwhile,there were significant differences between two groups of 30min,2h,4h,8h,12h(t =5.208,2.447,2.449,5.959,5.959,P =0.001,0.049,0.042,0.000,0.000),but there was no significant difference after 24h.Conclusion There was significant doses-effect relationship between serum nickel content of acute nickel carbonyl poisoning rats and the dosage.Nickel carbonyl or its metabolites were excreted mainly within 24h.
