CAJ | 학술논문

目的分别采用卤素灯光源共焦显微镜和激光光源共焦显微镜对中国正常成人活体角膜各层组织结构进行观察,对照研究其图像特征.方法对35例(70只眼)中国成人(年龄18～55岁)中央部角膜分别采用卤素灯光源共焦显微镜和激光光源共焦显微镜进行检查,研究角膜各层结构的图像特点,并进行对比.结果共焦显微镜检查的35例(70只眼)中,67只眼95.7％成功获得了角膜上皮翼状细胞层的图像,70只眼100％成功获得角膜上皮基底细胞层、前弹力层、前基质层、后基质层及角膜内皮细胞层的图像.结论卤素灯光源共焦显微镜的放大倍数是1000倍,细胞放大倍数大,近似于初级电镜的放大倍数,但卤素灯光源的组织穿透力较弱,光源易衰减,所得的角膜各层细胞图像较激光角膜共焦显微镜的略微模糊,所得角膜各层图像总体颜色偏灰、结构偏模糊.激光角膜共焦显微镜的放大倍数是800倍,较卤素灯光源的共焦显微镜略小,但激光光源的组织穿透力强,所得角膜各层细胞图像较卤素灯光源的更清晰,而且在特定角度可获得非常类似角膜组织病理学切片的图像.
목적 분별채용서소등광원공초현미경화격광광원공초현미경대중국정상성인활체각막각층조직결구진행관찰,대조연구기도상특정.방법 대35례(70지안)중국성인(년령18～55세)중앙부각막분별채용서소등광원공초현미경화격광광원공초현미경진행검사,연구각막각층결구적도상특점,병진행대비.결과 공초현미경검사적35례(70지안)중,67지안95.7％성공획득료각막상피익상세포층적도상,70지안100％성공획득각막상피기저세포층、전탄력층、전기질층、후기질층급각막내피세포층적도상.결론 서소등광원공초현미경적방대배수시1000배,세포방대배수대,근사우초급전경적방대배수,단서소등광원적조직천투력교약,광원역쇠감,소득적각막각층세포도상교격광각막공초현미경적략미모호,소득각막각층도상총체안색편회、결구편모호.격광각막공초현미경적방대배수시800배,교서소등광원적공초현미경략소,단격광광원적조직천투력강,소득각막각층세포도상교서소등광원적경청석,이차재특정각도가획득비상유사각막조직병이학절편적도상.
Objective To observe the different organizational structures of corneal layers of normal Chinese adult by halogen light source confocal microscopy and laser light source confocal microscopy in vivo,to study and compare its image features.Methods The central parts of the cornea were examined by halogen light source confocal microscopy and laser light source confocal microscopy from 35 cases (70 eyes) adult (age from 18 to 55 years old).The image characteristics of organizational structures of different corneal layers were studied and compared.Results The corneal epithelial wing-like cell layer images of 67 eyes (95.7％) were successfully examined.The basal epithelium,Bowman's membrane,anterior stroma,posterior stroma,Dscemet's membrane,endothelium were successful examined 100％.Conclusions The magnification of halogen light confocal microscopy are 1000 times,and the better magnification of cells.It's similar to the magnification of the primary electron microscopy.Because of the weak tissue penetration of the halogen light source and easy to decay,the cell images of different corneal layers of halogen light confocal microscopy are more blurred than laser corneal confocal microscopy,and the color of that are more gray,the structure of that are partial fuzzy; The magnification of laser corneal confocal microscopy are 800 times,cell magnification of that are slightly smaller than that of halogen light confocal microscopy.The cell images of different corneal layers of laser corneal confocal microscopy are more clearly than that of halogen light confocal microscopy,because of the stronger tissue penetration of the laser light source.And the very similar to the corneal tissue pathology slice images are available at a particular point of view.