中国实用眼科杂志
中國實用眼科雜誌
중국실용안과잡지
CHINESE JOURNAL OF PRACTICAL OPHTHALMOLOGY
2012年
11期
1321-1324
,共4页
耿祥娥%赵玉斌%张少华%吕杰%徐治伟%王金清
耿祥娥%趙玉斌%張少華%呂傑%徐治偉%王金清
경상아%조옥빈%장소화%려걸%서치위%왕금청
EphB4%EphrinB2%视网膜母细胞瘤%免疫组织化学
EphB4%EphrinB2%視網膜母細胞瘤%免疫組織化學
EphB4%EphrinB2%시망막모세포류%면역조직화학
EphB4%EphrinB2%Retinoblastoma%Immunohistochemistry
目的 探讨EphB4和EphrinB2的蛋白在视网膜母细胞瘤中的表达及其意义.方法 应用免疫组织化学SP法测定10例正常视网膜组织和40例视网膜母细胞瘤标本中EphB4和EphrinB2的表达水平,分析其与性别、临床分期、分化程度和视神经浸润的关系.结果(1)EphB4和EphrinB2蛋白表达的阳性率,在正常视网膜组织中分别为5％和2.5％,40例视网膜母细胞瘤标本中分别为77.5％和67.5％.EphB4和EphrinB2在视网膜母细胞瘤组织中的阳性率与显著高于正常视网膜组织中的阳性率(P＜0.05).(2)EphB4和EphrinB2的蛋白在视网膜母细胞瘤的阳性表达率与肿瘤组织的临床分期、分化程度及视神经浸润显著相关(P＜0.05).(3)EphB4和EphrinB2的蛋白在视网膜母细胞瘤组织中的阳性表达显著相关(P＜0.05).结论 EphB4和EphrinB2可能在视网膜母细胞瘤的发生和发展过程中起重要作用,可作为评价视网膜母细胞瘤的浸润及预后的标志物.
目的 探討EphB4和EphrinB2的蛋白在視網膜母細胞瘤中的錶達及其意義.方法 應用免疫組織化學SP法測定10例正常視網膜組織和40例視網膜母細胞瘤標本中EphB4和EphrinB2的錶達水平,分析其與性彆、臨床分期、分化程度和視神經浸潤的關繫.結果(1)EphB4和EphrinB2蛋白錶達的暘性率,在正常視網膜組織中分彆為5％和2.5％,40例視網膜母細胞瘤標本中分彆為77.5％和67.5％.EphB4和EphrinB2在視網膜母細胞瘤組織中的暘性率與顯著高于正常視網膜組織中的暘性率(P＜0.05).(2)EphB4和EphrinB2的蛋白在視網膜母細胞瘤的暘性錶達率與腫瘤組織的臨床分期、分化程度及視神經浸潤顯著相關(P＜0.05).(3)EphB4和EphrinB2的蛋白在視網膜母細胞瘤組織中的暘性錶達顯著相關(P＜0.05).結論 EphB4和EphrinB2可能在視網膜母細胞瘤的髮生和髮展過程中起重要作用,可作為評價視網膜母細胞瘤的浸潤及預後的標誌物.
목적 탐토EphB4화EphrinB2적단백재시망막모세포류중적표체급기의의.방법 응용면역조직화학SP법측정10례정상시망막조직화40례시망막모세포류표본중EphB4화EphrinB2적표체수평,분석기여성별、림상분기、분화정도화시신경침윤적관계.결과(1)EphB4화EphrinB2단백표체적양성솔,재정상시망막조직중분별위5％화2.5％,40례시망막모세포류표본중분별위77.5％화67.5％.EphB4화EphrinB2재시망막모세포류조직중적양성솔여현저고우정상시망막조직중적양성솔(P＜0.05).(2)EphB4화EphrinB2적단백재시망막모세포류적양성표체솔여종류조직적림상분기、분화정도급시신경침윤현저상관(P＜0.05).(3)EphB4화EphrinB2적단백재시망막모세포류조직중적양성표체현저상관(P＜0.05).결론 EphB4화EphrinB2가능재시망막모세포류적발생화발전과정중기중요작용,가작위평개시망막모세포류적침윤급예후적표지물.
Objective To investigate the protein expressions of EphB4 and EphrinB2 in retinoblastoma and to study their significance.Methods The expression of EphB4 and EphrinB2 was examined immunohistochemistrically by SP method in tissues of 10 normal retina and 40 patients with retinoblastoma and to study their relationship with gender,clinical stage,differentiation and optic nerve infiltration.Results 1.The protein expression of EphB4 and EphrinB2 observed in the normal tissues of retina was 5％ and 2.5％ respectively.In the 40 retinoblastoma cases,the positive rate of them was 77.5％ and 67.5％ respectively.The positive rate of EphB4 and EphrinB2 in retinoblastoma was higher than that in the normal retina(P＜0.05).2.The positive expression of EphB4 and EphrinB2 protein were associated with clinical stage,differentiation and optic nerve infiltration(P＜0.05).3.The positive expression of EphB4 and EphrinB2 protein in retinoblastoma was positively correlated(P＜0.05).Conclusions EphB4 and EphrinB2 play important roles in carcinogenesis,development procedures of retinoblastoma and might be served as objective indicators of invasion and prognosis of retinoblastoma.
