中国实用眼科杂志
中國實用眼科雜誌
중국실용안과잡지
CHINESE JOURNAL OF PRACTICAL OPHTHALMOLOGY
2012年
11期
1377-1380
,共4页
PDGF-α受体%沉默%人晶状体上皮细胞%增殖%凋亡
PDGF-α受體%沉默%人晶狀體上皮細胞%增殖%凋亡
PDGF-α수체%침묵%인정상체상피세포%증식%조망
PDGF-α receptor%Silence%Lens epithelial cells%Proliferation%Apoptosis
目的 研究血小板源性生长因子-α受体沉默对人晶状体上皮细胞增殖及凋亡的影响,为治疗后发性白内障提供实验依据.方法 体外培养人晶状体上皮细胞SRA01/04,用脂质体将合成的血小板源性生长因子-α受体反义寡核苷酸(PDGFR-αASODN)处理SRA01/04,MTT法检测细胞的增殖;流式细胞仪检测细胞周期及细胞凋亡;Hoechst33258荧光染料染色法分析细胞凋亡;RT-PCR检测PDGF-α受体的表达.结果 PDGFR-αASODN作用于SRA01/04细胞24 h～72 h,SRA01/04细胞增殖受抑制,且72 h时对细胞的抑制作用最明显,与低浓度药物组比较,高浓度药物组对细胞的抑制作用增强(P＜0.05);实验组细胞凋亡率分别为(3.22±0.25)％、(5.29±0.27)％,与对照组(0.75±0.67)％和错义寡核苷酸组(1.46±0.60)％比较,差异有统计学意义(P＜0.05);实验组G1期细胞分布率分别为(53.31±1.30)％、(59.98±0.95)％,与对照组(47.73±1.18)％和错义寡核苷酸组(49.48±1.09)％比较,差异有统计学意义(P＜0.05);实验组PDGF-α受体在SRA01/04中表达下调.结论 PDGF-α受体沉默能抑制人晶状体上皮细胞的增殖,诱导其凋亡.
目的 研究血小闆源性生長因子-α受體沉默對人晶狀體上皮細胞增殖及凋亡的影響,為治療後髮性白內障提供實驗依據.方法 體外培養人晶狀體上皮細胞SRA01/04,用脂質體將閤成的血小闆源性生長因子-α受體反義寡覈苷痠(PDGFR-αASODN)處理SRA01/04,MTT法檢測細胞的增殖;流式細胞儀檢測細胞週期及細胞凋亡;Hoechst33258熒光染料染色法分析細胞凋亡;RT-PCR檢測PDGF-α受體的錶達.結果 PDGFR-αASODN作用于SRA01/04細胞24 h～72 h,SRA01/04細胞增殖受抑製,且72 h時對細胞的抑製作用最明顯,與低濃度藥物組比較,高濃度藥物組對細胞的抑製作用增彊(P＜0.05);實驗組細胞凋亡率分彆為(3.22±0.25)％、(5.29±0.27)％,與對照組(0.75±0.67)％和錯義寡覈苷痠組(1.46±0.60)％比較,差異有統計學意義(P＜0.05);實驗組G1期細胞分佈率分彆為(53.31±1.30)％、(59.98±0.95)％,與對照組(47.73±1.18)％和錯義寡覈苷痠組(49.48±1.09)％比較,差異有統計學意義(P＜0.05);實驗組PDGF-α受體在SRA01/04中錶達下調.結論 PDGF-α受體沉默能抑製人晶狀體上皮細胞的增殖,誘導其凋亡.
목적 연구혈소판원성생장인자-α수체침묵대인정상체상피세포증식급조망적영향,위치료후발성백내장제공실험의거.방법 체외배양인정상체상피세포SRA01/04,용지질체장합성적혈소판원성생장인자-α수체반의과핵감산(PDGFR-αASODN)처리SRA01/04,MTT법검측세포적증식;류식세포의검측세포주기급세포조망;Hoechst33258형광염료염색법분석세포조망;RT-PCR검측PDGF-α수체적표체.결과 PDGFR-αASODN작용우SRA01/04세포24 h～72 h,SRA01/04세포증식수억제,차72 h시대세포적억제작용최명현,여저농도약물조비교,고농도약물조대세포적억제작용증강(P＜0.05);실험조세포조망솔분별위(3.22±0.25)％、(5.29±0.27)％,여대조조(0.75±0.67)％화착의과핵감산조(1.46±0.60)％비교,차이유통계학의의(P＜0.05);실험조G1기세포분포솔분별위(53.31±1.30)％、(59.98±0.95)％,여대조조(47.73±1.18)％화착의과핵감산조(49.48±1.09)％비교,차이유통계학의의(P＜0.05);실험조PDGF-α수체재SRA01/04중표체하조.결론 PDGF-α수체침묵능억제인정상체상피세포적증식,유도기조망.
Objective To investigate the effect of PDGF-α receptor silence on proliferation and apoptosis of human lens epithelial cell in vitro,and to provide the treatment method for posterior capsule opacification.Methods Lens epithelial cells were cultured in vitro and PDGFR-α receptor antisense oligonucleotides was transfected into cells by cationic liposomes,cell proliferation was detected by MTT experiment,cell apoptosis by Hoechst 33258,cell cycle and apoptosis by Flow Cytometer,and gene expression by one step Reverse transcription Polymerase Chain Response(RT-PCR).Results PDGF-α receptor antisense oligonucleotides had effect on SRA01/04 cells 24h-72h,and among all of them SRA01/04 cell 72h expressed most significant inhibitory effects,compared with the low concentration of the drug group,the high concentration of the drug group had stronger effect on inhabitation(P＜0.05);the SRA01/04 cells apoptosis ratio of experimental group were(3.22±0.25)％ and(5.29±0.27)％,showing significance with control group(0.75±0.67)％ and missense oligonucleotide group(146±0.60)％(P＜0.05);percentage of cells in G1 phase in experimental group were(53.31±1.30)％ and(59.98±0.95)％,showing significance with control group(47.73±1.18)％ and missense oligonucleotide group(49.48±1.09)％(P＜0.05);but the experimental group of PDGF-α receptor had weak expression in SRA01/04.Conclusions PDGF-α receptor silence can inhibit human lens epithelial cell proliferation and induce apoptosis.
