CAJ | 학술논문

目的通过甘油醛与葡萄糖滴眼,意在评估糖基化交联对大鼠角膜生物力学特性的影响.方法实验研究.将25只SD大鼠随机分为五组,每组五只,两组分别给予浓度为0.005 mol/L、0.05 mol/L.的甘油醛,另两组分别给予浓度为0.5％、5％的葡萄糖滴右眼,每天2次,第五组为空白对照.7天后处死大鼠,应用微材料力学性能测试系统进行生物力学条带实验比较极限应力σ max(MPa),极限应变εmax(％),6％弹性模量E(MPs),并观察大鼠角膜的透明度和组织学检查.结果甘油醛滴药组大鼠角膜生物力学强度明显增加.对照组角膜极限应力、极限应变、6％弹性模量分别为(2.053±0.853) MPa、(43.87％±1.89)％、(3.252±1.717) MPa.大鼠角膜极限应力0.005 mol/L、0.05 mol/L甘油醛组分别为(6.043±2.084) MPa (P =0.026)、(10.759±3.337) MPa (P =0.000);0.5％、5％葡萄糖组分别为(2.101±0.463) MPa (P =0.976)、(2.373±0.569) MPa (P =0.847).四个实验组的极限应变分别为(36.57％±3.09)％(P =0.276)、(28.53±1.89)％(P =0.009)、(34.80±8.14)％(P=0.090)和(34.32％±4.33)％(P=0.072).6％弹性模量分别为(7.718±1.076) MPa (P=0.004)、(14.102±4.011) MPa (P =0.000)、(3.927±0.314) MPa (P =0.629)、(5.492±0.659) MPa(P =0.120).组织学检查提示甘油醛滴药组胶原纤维间隙变窄,且与浓度有一定相关性,葡萄糖滴药组胶原纤维密度变化不大,5％葡萄糖组角膜上皮出现水肿,而透明度无明显变化.结论糖基化交联可以有效促进角膜胶原交联,增加其生物力学强度.甘油醛交联力比葡萄糖强,其作为治疗圆锥角膜的交联剂有待于进一步的动物实验和临床研究.
목적 통과감유철여포도당적안,의재평고당기화교련대대서각막생물역학특성적영향.방법 실험연구.장25지SD대서수궤분위오조,매조오지,량조분별급여농도위0.005 mol/L、0.05 mol/L.적감유철,령량조분별급여농도위0.5％、5％적포도당적우안,매천2차,제오조위공백대조.7천후처사대서,응용미재료역학성능측시계통진행생물역학조대실험비교겁한응력σ max(MPa),겁한응변εmax(％),6％탄성모량E(MPs),병관찰대서각막적투명도화조직학검사.결과 감유철적약조대서각막생물역학강도명현증가.대조조각막겁한응력、겁한응변、6％탄성모량분별위(2.053±0.853) MPa、(43.87％±1.89)％、(3.252±1.717) MPa.대서각막겁한응력0.005 mol/L、0.05 mol/L감유철조분별위(6.043±2.084) MPa (P =0.026)、(10.759±3.337) MPa (P =0.000);0.5％、5％포도당조분별위(2.101±0.463) MPa (P =0.976)、(2.373±0.569) MPa (P =0.847).사개실험조적겁한응변분별위(36.57％±3.09)％(P =0.276)、(28.53±1.89)％(P =0.009)、(34.80±8.14)％(P=0.090)화(34.32％±4.33)％(P=0.072).6％탄성모량분별위(7.718±1.076) MPa (P=0.004)、(14.102±4.011) MPa (P =0.000)、(3.927±0.314) MPa (P =0.629)、(5.492±0.659) MPa(P =0.120).조직학검사제시감유철적약조효원섬유간극변착,차여농도유일정상관성,포도당적약조효원섬유밀도변화불대,5％포도당조각막상피출현수종,이투명도무명현변화.결론 당기화교련가이유효촉진각막효원교련,증가기생물역학강도.감유철교련력비포도당강,기작위치료원추각막적교련제유대우진일보적동물실험화림상연구.
Objective To evaluate the biomechanical effects of glyceraldehyde and glucose induced glycation collagen crosslinking on rats corneas.Methods Twenty-five SD rats were randomly divided into 5 groups.Each group had 5 rats.Four groups of them were respectively treated with 0.005mol/L,0.05mol/L glyceraldhyde,0.5％,5％ glucose drops into the right eye 2 times per day.Seven days later,then the rats were humanely killed.Biomechanical stress-sWain measurements of corneal strips were performed using a microcomputer-controlled biomaterial testing device.In addition,transparency of corneal buttons and histological examination by light microscopy were conducted.Results After the crosslinking treatment,the Ultimate stress,the Ultimate sWain,6％ Elastic Modulus were 2.053±0.853MPa,43.87％±1.89％ and 3.252±l.717MPa in the controls.The Ultimate stress was 6.043±2.084MPa (P =0.026),10.759±3.337MPa (P=0.000),2.101±0.463MPa (P =0.976),2.373±0.569MPa (P =0.847) with glyceraldehyde at concentrations of 0.005mol/L,0.05 mol/L,glucose at concentrations of 0.5％,5％ respectively.The Ultimate strain was 36.57％±3.09％ (P =0.276),28.53％±1.89％ (P =0.009),34.80％±8.14％ (P =0.090),34.32％±4.33％ (P =0.072) in the four treated groups.6％ Elastic Modulus was 7.718±1.076MPa (P =0.004),14.102±4.011MPa (P =0.000)with glyceraldehyde at concentrations of 0.005mol/L,0.05 mol/L; 3.927±0.314MPa (P =0.629),5.492±0.659MPa (P =0.120) with glucose at concentrations of 0.5％,5％.A decreased interlamellar space in treated corneas was also observed.Transparency of corneas had no significant difference between the five groups.Conclusions Glycation-induced crosslinking is an effective treatment for corneal collagen crosslinking,producing an increase in biomechanical strength.Glyceraldehyde as a more effective crosslinker agent leads to a rather significantly increase of mechanical stiffness in rats corneas.Further animal and clinical studies should to be tested.