中国实用眼科杂志
中國實用眼科雜誌
중국실용안과잡지
CHINESE JOURNAL OF PRACTICAL OPHTHALMOLOGY
2013年
7期
880-883
,共4页
何宇%石晶明%贾松柏%唐罗生
何宇%石晶明%賈鬆柏%唐囉生
하우%석정명%가송백%당라생
急性前葡萄膜炎%小柳原田综合征%单核细胞趋化蛋白-1%酶联免疫吸附试验
急性前葡萄膜炎%小柳原田綜閤徵%單覈細胞趨化蛋白-1%酶聯免疫吸附試驗
급성전포도막염%소류원전종합정%단핵세포추화단백-1%매련면역흡부시험
AAU%VKH%MCP-1%ELISA
目的 检测单核细胞趋化蛋白-1(MCP-1)在急性前葡萄膜炎(AAU)及小柳原田综合征(VKH)患者血清中的表达,探讨其在葡萄膜炎发病中的可能机制.方法 收集62例葡萄膜炎患者(AAU患者43例、VKH患者19例),14例正常对照组,提取血清.将AAU患者分为HLA-B27阳性组17例和HLA-B27阴性组26例;并将VKH患者就诊时是否已使用糖皮质激素治疗分为未使用激素组11例和已使用激素组8例.采用ELISA法检测各组血清中MCP-1的含量,对其进行统计分析.结果 AAU患者HLA-B27阳性组与阴性组血清中MCP-1浓度分别为(115.220±22.698)pg/ml、(102.210±38.689)pg/ml,与正常对照组血清MCP-1浓度(107.900±21.655) pg/ml比较差异无统计学意义(P＞0.05).未使用激素治疗的VKH患者外周血清中MCP-1含量(217.370±38.751) pg/ml较正常组显著增高(P＜0.05).激素治疗VKH组MCP-1含量(195.480±19.977) pg/ml略低于未治疗组(P ＞0.05),但仍高于正常组(P＜0.05).结论 与正常对照组比较,AAU患者血清MCP-1的表达无明显改变;而VKH患者血清MCP-1的表达上调,推测其可能参与了VKH的病理生理过程,全身使用糖皮质激素可能通过抑制MCP-1表达控制眼部炎症.
目的 檢測單覈細胞趨化蛋白-1(MCP-1)在急性前葡萄膜炎(AAU)及小柳原田綜閤徵(VKH)患者血清中的錶達,探討其在葡萄膜炎髮病中的可能機製.方法 收集62例葡萄膜炎患者(AAU患者43例、VKH患者19例),14例正常對照組,提取血清.將AAU患者分為HLA-B27暘性組17例和HLA-B27陰性組26例;併將VKH患者就診時是否已使用糖皮質激素治療分為未使用激素組11例和已使用激素組8例.採用ELISA法檢測各組血清中MCP-1的含量,對其進行統計分析.結果 AAU患者HLA-B27暘性組與陰性組血清中MCP-1濃度分彆為(115.220±22.698)pg/ml、(102.210±38.689)pg/ml,與正常對照組血清MCP-1濃度(107.900±21.655) pg/ml比較差異無統計學意義(P＞0.05).未使用激素治療的VKH患者外週血清中MCP-1含量(217.370±38.751) pg/ml較正常組顯著增高(P＜0.05).激素治療VKH組MCP-1含量(195.480±19.977) pg/ml略低于未治療組(P ＞0.05),但仍高于正常組(P＜0.05).結論 與正常對照組比較,AAU患者血清MCP-1的錶達無明顯改變;而VKH患者血清MCP-1的錶達上調,推測其可能參與瞭VKH的病理生理過程,全身使用糖皮質激素可能通過抑製MCP-1錶達控製眼部炎癥.
목적 검측단핵세포추화단백-1(MCP-1)재급성전포도막염(AAU)급소류원전종합정(VKH)환자혈청중적표체,탐토기재포도막염발병중적가능궤제.방법 수집62례포도막염환자(AAU환자43례、VKH환자19례),14례정상대조조,제취혈청.장AAU환자분위HLA-B27양성조17례화HLA-B27음성조26례;병장VKH환자취진시시부이사용당피질격소치료분위미사용격소조11례화이사용격소조8례.채용ELISA법검측각조혈청중MCP-1적함량,대기진행통계분석.결과 AAU환자HLA-B27양성조여음성조혈청중MCP-1농도분별위(115.220±22.698)pg/ml、(102.210±38.689)pg/ml,여정상대조조혈청MCP-1농도(107.900±21.655) pg/ml비교차이무통계학의의(P＞0.05).미사용격소치료적VKH환자외주혈청중MCP-1함량(217.370±38.751) pg/ml교정상조현저증고(P＜0.05).격소치료VKH조MCP-1함량(195.480±19.977) pg/ml략저우미치료조(P ＞0.05),단잉고우정상조(P＜0.05).결론 여정상대조조비교,AAU환자혈청MCP-1적표체무명현개변;이VKH환자혈청MCP-1적표체상조,추측기가능삼여료VKH적병리생리과정,전신사용당피질격소가능통과억제MCP-1표체공제안부염증.
Objective To explore the effect of monocyte chemoattractant protein-1 (MCP-1) in uveitis pathogenetic mechanism by detecting the concentration of MCP-1 in serum of AAU and VKH.Methods Sixty-two patients suffered uveitis were collected (43 AAU patients,19 VKH patients),and 14 healthy individuals served as control group.Patients with AAU were divided into HLA-B27 (+) group and HLA-B27 (-) group.Meanwhile,patients with VKH were divided into two groups according to whether steroid was administered systematically or not (VKH patients who had administered steroid systematically (group A); patients without any systemic therapy of steroid (group B).The level of serum MCP-1 was detected by ELISA.Statistic analysis was performed to compare the difference between groups.Results Serum concentration of MCP-1 in HLA-B27 positive AAU,HLA-B27 negative AAU and control group were 115.220±22.698 pg/ml,102.210±38.689 pg/ml,107.900±21.655 pg/ml respectively,there were no significant difference among three groups (P ＞0.05).The concentration of MCP-1 in VKH group B (217.370±38.751 pg/ml) was much higher than that of control group (P ＞0.05).The concentration of MCP-1 in VKH group A (195.480±19.977 pg/ml) decreased slightly when compared with group B,but still higher than that of control group (P ＞0.05).Conclusions There is no change of the expression of MCP-1 in AAU.While the increased MCP-1 in VKH patient serum may participate in the pathogenesis of VKH,and steroid may suppress ocular inflammation by inhibiting the production of MCP-1.
