CAJ | 학술논문

目的对真菌性角膜炎的病原菌变化及实验室诊断进行总结,为该病的诊断治疗提供可靠的依据.方法分析2008～2012年5年间在唐山市眼科医院拟诊为真菌性角膜炎的病例530份,统计其涂片Giemsa染色细胞学检查及真菌培养阳性率,应用卡方检验和百分比统计方法,将这些资料与1999～2003年的材料进行比较,并分析其发病的季节性分布规律.结果在530份标本中涂片Giemsa染色细胞学检查发现真菌菌丝233例,阳性率43.97％,真菌培养阳性296例,阳性率55.84％.且发病季节多集中于9～12月份,占60.6％.2008～2012年镰孢菌属、无孢菌属,曲霉菌属,链格孢菌属分别是前四位的致病菌属,各占:67.24％,19.26％,7.43％和5.07％.结论近十年就诊的真菌性角膜炎患者呈增加趋势;涂片染色法是快速诊断可疑真菌性角膜炎的首选方法;镰孢菌属是我地区真菌性角膜炎的主要致病菌属,占67.24％;无孢菌属和曲霉菌属呈上升趋势,链格孢菌属呈下降趋势.
목적 대진균성각막염적병원균변화급실험실진단진행총결,위해병적진단치료제공가고적의거.방법 분석2008～2012년5년간재당산시안과의원의진위진균성각막염적병례530빈,통계기도편Giemsa염색세포학검사급진균배양양성솔,응용잡방검험화백분비통계방법,장저사자료여1999～2003년적재료진행비교,병분석기발병적계절성분포규률.결과 재530빈표본중도편Giemsa염색세포학검사발현진균균사233례,양성솔43.97％,진균배양양성296례,양성솔55.84％.차발병계절다집중우9～12월빈,점60.6％.2008～2012년렴포균속、무포균속,곡매균속,련격포균속분별시전사위적치병균속,각점:67.24％,19.26％,7.43％화5.07％.결론 근십년취진적진균성각막염환자정증가추세;도편염색법시쾌속진단가의진균성각막염적수선방법;렴포균속시아지구진균성각막염적주요치병균속,점67.24％;무포균속화곡매균속정상승추세,련격포균속정하강추세.
Objective To analyze the macroscopical shifting trends and laboratory diagnosis of pathogenic bacteria in fungal keratitis,to provide a dependable basis for the diagnosis and treatment of the disease.Methods This study retrospectively reviewed 530 cases that diagnosed as fungal keratitis in Tangshan ophthalmic hospital between 2008 and 2012,and statistic analyzed their smear Giemsa stain cytology,fungal culture.These data was compared by using chi-square test and percentages,and compared those with data in the period of 1999-2003.Its seasonal distribution of the disease was analyzed.Results Of 233 cases of fungal hyphae from the 530 samples were revealed,through Giemsa stained smear cytology examination,and the positive rate was 43.97％,and 296 cases of fungal culture was positive,the positive rate 55.84％.And the season of incidence focused on the 9-12 month,accounted for 60.6％.In 2008-2012 Fusarium species,No-spore species,Aspergillus,Altemaria species were the top four pathogens,respectively,accounted for 67.24％,19.26％,7.43％ and 5.07％ respectively.Conclusions Fungal keratitis cases need treatment in our hospital is increasing in recent 10 years.Smear staining method is a rapid diagnosis method of suspected fungal keratitis.Fusarium species is the major fungal pathogens in our area,accounting for 67.24％ and is always the first pathogen.Aspergillus species and no-spore species are uptrend,however Alternaria species decline.