中国实用眼科杂志
中國實用眼科雜誌
중국실용안과잡지
CHINESE JOURNAL OF PRACTICAL OPHTHALMOLOGY
2013年
10期
1357-1360
,共4页
硅油%血视网膜屏障%冷冻%白介素-6
硅油%血視網膜屏障%冷凍%白介素-6
규유%혈시망막병장%냉동%백개소-6
Silicone oil%Cryotherapy%Blood retinal barrier%IL-6
目的 建立血视网膜屏障破坏的兔眼模型,对比研究硅油填充和长效气体填充后对前房水蛋白和白介素-6(IL-6)水平的影响.方法 进行标准三通道玻璃体切除,通过冷凝破坏血视网膜屏障,玻璃体腔内填充硅油(硅油组)或长效气体(C3F8组)和平衡盐溶液(对照组),每组各10只眼.术后14天和28天抽取前房水Elisa方法检测蛋白和白介素-6水平.结果 术后14天,硅油组(11.24±1.36) mg/ml和气体组(9.52±0.99) mg/ml前房水蛋白水平差异无统计学意义(P＞0.05).术后28天,硅油组前房水蛋白水平(6.27±0.88) mg/ml明显高于气体组(2.80±0.85) mg/ml和对照组(2.78±0.72) mg/ml,且差异具有统计学意义(P＜0.05).术后14及28天,硅油组前房水IL-6水平(647.71±87.26) pg/ml,(392.98±32.4) pg/ml均明显高于气体组(523.46±43.02) pg/ml,(85.47±14.03) pg/ml和对照组(509.47±52.63) pg/ml,(72.43±13.9) pg/ml,且差异具有统计学意义(P＜0.05).结论 血视网膜屏障破坏后,硅油填充可造成炎症反应和血视网膜屏障修复的延迟.
目的 建立血視網膜屏障破壞的兔眼模型,對比研究硅油填充和長效氣體填充後對前房水蛋白和白介素-6(IL-6)水平的影響.方法 進行標準三通道玻璃體切除,通過冷凝破壞血視網膜屏障,玻璃體腔內填充硅油(硅油組)或長效氣體(C3F8組)和平衡鹽溶液(對照組),每組各10隻眼.術後14天和28天抽取前房水Elisa方法檢測蛋白和白介素-6水平.結果 術後14天,硅油組(11.24±1.36) mg/ml和氣體組(9.52±0.99) mg/ml前房水蛋白水平差異無統計學意義(P＞0.05).術後28天,硅油組前房水蛋白水平(6.27±0.88) mg/ml明顯高于氣體組(2.80±0.85) mg/ml和對照組(2.78±0.72) mg/ml,且差異具有統計學意義(P＜0.05).術後14及28天,硅油組前房水IL-6水平(647.71±87.26) pg/ml,(392.98±32.4) pg/ml均明顯高于氣體組(523.46±43.02) pg/ml,(85.47±14.03) pg/ml和對照組(509.47±52.63) pg/ml,(72.43±13.9) pg/ml,且差異具有統計學意義(P＜0.05).結論 血視網膜屏障破壞後,硅油填充可造成炎癥反應和血視網膜屏障脩複的延遲.
목적 건립혈시망막병장파배적토안모형,대비연구규유전충화장효기체전충후대전방수단백화백개소-6(IL-6)수평적영향.방법 진행표준삼통도파리체절제,통과냉응파배혈시망막병장,파리체강내전충규유(규유조)혹장효기체(C3F8조)화평형염용액(대조조),매조각10지안.술후14천화28천추취전방수Elisa방법검측단백화백개소-6수평.결과 술후14천,규유조(11.24±1.36) mg/ml화기체조(9.52±0.99) mg/ml전방수단백수평차이무통계학의의(P＞0.05).술후28천,규유조전방수단백수평(6.27±0.88) mg/ml명현고우기체조(2.80±0.85) mg/ml화대조조(2.78±0.72) mg/ml,차차이구유통계학의의(P＜0.05).술후14급28천,규유조전방수IL-6수평(647.71±87.26) pg/ml,(392.98±32.4) pg/ml균명현고우기체조(523.46±43.02) pg/ml,(85.47±14.03) pg/ml화대조조(509.47±52.63) pg/ml,(72.43±13.9) pg/ml,차차이구유통계학의의(P＜0.05).결론 혈시망막병장파배후,규유전충가조성염증반응화혈시망막병장수복적연지.
Objective To evaluate the aqueous protein and IL-6 levels induced by cryotherapy in rabbit eyes filled with silicone oil and C3F8.Methods The rabbit eyes were filled with silicone oil,C3F8 or BSS (control group) after vitrectomy and cryotherapy.Protein and IL-6 levels were examined by ELISA and BCA on post-op day 14 and 28.Results The IL-6 levels in silicone oil group (647.71±87.26) pg/ml,(392.98±32.4)pg/ml were significantly higher than those in C3F8 (523.46±43.02)pg/ml,(85.47±14.03)pg/ml and control group (72.43±13.9)pg/ml,(72.43±13.9)pg/ml on post-op day 14 and 28 (P ＜0.05).The protein levels in silicone oil and gas group showed no significant difference on post-op day14.The protein levels in silicone oil (6.27±0.88)mg/ml and gas group (2.80±0.85)mg/ml showed statistically significant difference on post-op day 28 (P ＜0.05).Conclusions Silicone oil tamponade delays BRB repair in rabbit eyes with cryotherapy.
