中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2013年
3期
242-246
,共5页
方洧靖%张荣欣%罗容珍%区庆坚%万德森%潘志忠%伍小军
方洧靖%張榮訢%囉容珍%區慶堅%萬德森%潘誌忠%伍小軍
방유정%장영흔%라용진%구경견%만덕삼%반지충%오소군
胃肠间质瘤%外科手术%生物标志物%肿瘤复发%预后
胃腸間質瘤%外科手術%生物標誌物%腫瘤複髮%預後
위장간질류%외과수술%생물표지물%종류복발%예후
Gastrointestinal stromal tumors%Surgical procedures%Biomarkers%Neoplasm recurrence%Prognosis
目的 探讨影响胃肠间质瘤(GIST)完全切除术后复发转移及预后的生物标志物.方法 收集1990年1月至2008年12月中山大学肿瘤防治中心148例完全切除术后的GIST标本,利用免疫组织化学技术、以组织芯片为载体,检测Ki-67、E-cadherin、MMP7、CD44、nm23、P53、Survivin、Cyclin D1、COX-2和VEGF共10个生物指标,研究其表达与GIST完全切除术后复发转移和预后的关系.结果 单因素预后分析(Log-rank检验)结果显示,Ki-67、E-cadherin、MMP7、CD44、P53和Survivin与GIST术后无瘤生存有关(均P＜0.05),Ki-67、E-cadherin、P53和Survivin与总体生存有关(均P＜0.05).多因素预后分析(Cox回归模型)结果显示,Ki-67、CD44和P53是影响GIST完全切除术后复发转移的独立因素(均P＜0.05);Ki-67是影响术后生存的独立因素(P＜0.05).结论 Ki-67、CD44和P53与GIST完全切除术后复发转移密切相关,Ki-67还能提示预后.
目的 探討影響胃腸間質瘤(GIST)完全切除術後複髮轉移及預後的生物標誌物.方法 收集1990年1月至2008年12月中山大學腫瘤防治中心148例完全切除術後的GIST標本,利用免疫組織化學技術、以組織芯片為載體,檢測Ki-67、E-cadherin、MMP7、CD44、nm23、P53、Survivin、Cyclin D1、COX-2和VEGF共10箇生物指標,研究其錶達與GIST完全切除術後複髮轉移和預後的關繫.結果 單因素預後分析(Log-rank檢驗)結果顯示,Ki-67、E-cadherin、MMP7、CD44、P53和Survivin與GIST術後無瘤生存有關(均P＜0.05),Ki-67、E-cadherin、P53和Survivin與總體生存有關(均P＜0.05).多因素預後分析(Cox迴歸模型)結果顯示,Ki-67、CD44和P53是影響GIST完全切除術後複髮轉移的獨立因素(均P＜0.05);Ki-67是影響術後生存的獨立因素(P＜0.05).結論 Ki-67、CD44和P53與GIST完全切除術後複髮轉移密切相關,Ki-67還能提示預後.
목적 탐토영향위장간질류(GIST)완전절제술후복발전이급예후적생물표지물.방법 수집1990년1월지2008년12월중산대학종류방치중심148례완전절제술후적GIST표본,이용면역조직화학기술、이조직심편위재체,검측Ki-67、E-cadherin、MMP7、CD44、nm23、P53、Survivin、Cyclin D1、COX-2화VEGF공10개생물지표,연구기표체여GIST완전절제술후복발전이화예후적관계.결과 단인소예후분석(Log-rank검험)결과현시,Ki-67、E-cadherin、MMP7、CD44、P53화Survivin여GIST술후무류생존유관(균P＜0.05),Ki-67、E-cadherin、P53화Survivin여총체생존유관(균P＜0.05).다인소예후분석(Cox회귀모형)결과현시,Ki-67、CD44화P53시영향GIST완전절제술후복발전이적독립인소(균P＜0.05);Ki-67시영향술후생존적독립인소(P＜0.05).결론 Ki-67、CD44화P53여GIST완전절제술후복발전이밀절상관,Ki-67환능제시예후.
Objective To explore the associated biomarkers influencing recurrence,metastasis and prognosis in patients with gastrointestinal stromal tumors (GIST) after complete resection.Methods Tumor tissue samples of 148 patients with GIST undergoing complete resection from January 1990 to December 2008 in Sun Yat-sen University Cancer Center were collected.The expressions of Ki-67,E-cadherin,MMP7,CD44,nm23,P53,survivin,Cyclin D1,COX-2,and VEGF in tumor tissue samples were detected by tissue microarray and immunohistochemistry (IHC).The association of above factors expressions with recurrence,metastasis and prognosis was examined.Results Log-rank test showed that Ki-67,E-cadherin,MMP7,CD44,P53 and survivin were associated to disease-free duration after complete GIST resection(all P＜0.05),and the Ki-67,E-cadherin,P53 and survivin were associated to overall survival (all P＜0.05).Cox multivariate analysis revealed that disease-free survival was associated with Ki-67,CD44 and P53 (all P＜0.05),and the overall survival was only associated with Ki-67(P＜0.05).Conclusion Ki-67,CD44 and P53 are closely associated with recurrence and metastasis after complete GIST resection,and Ki-67 can predict the prognosis of GIST.
