中国危重病急救医学
中國危重病急救醫學
중국위중병급구의학
CHINESE CRITICAL CARE MEDICINE
2012年
12期
730-733
,共4页
骨保护素%急性冠脉综合征%基因多态性
骨保護素%急性冠脈綜閤徵%基因多態性
골보호소%급성관맥종합정%기인다태성
Osteoprotegerin%Acute coronary syndrome%Gene polymorphism
目的 探讨福建地区汉族人群骨保护素(OPG)基因209G/A、245T/G、1181G/C位点基因多态性与急性冠脉综合征(ACS)及其病变程度的相关性.方法 采用前瞻性研究方法,选取2010年7月至2011年9月252例福建地区无血缘关系的汉族人为研究对象,纳入的184例ACS患者再根据冠状动脉(冠脉)造影结果分为单支病变组(60例)、双支病变组(68例)、多支病变组(54例),其中2例造影冠脉无狭窄；以68例建康体检者为对照.取静脉血提取DNA,采用Sanger测序法检测各组OPG基因209G/A、245T/G、1181G/C位点多态性.分析ACS组与对照组之间以及ACS不同病变程度组之间209G/A、245T/G、1181G/C各基因型和等位基因的分布频率以及基因多态性与ACS病变程度的关系.结果 ACS组与对照组OPG 209G/A、245T/G位点基因型及等位基因分布均无明显差异；而1181G/C位点基因型分布[GG型:92(50.0％)比43(63.2％),CC型:26(14.1％)比0,GC型:66(35.9％)比25 (36.8％),x2=11.240,P=0.004]、等位基因分布[等位基因G:250(67.9％)比111(81.6％),等位基因C:118(32.1％)比25(18.4％),x2=9.148,P=0.002]差异均有统计学意义.进一步分析发现,ACS单支病变组、双支病变组及多支病变组209G/A、245T/G及1181G/C位点分布比较均无明显差异.结论 福建地区汉族人群OPG 209G/A、245T/G位点多态性与ACS无明显相关性；1181G/C位点基因多态性与ACS发病之间存在相关性,但与其病变程度无关.
目的 探討福建地區漢族人群骨保護素(OPG)基因209G/A、245T/G、1181G/C位點基因多態性與急性冠脈綜閤徵(ACS)及其病變程度的相關性.方法 採用前瞻性研究方法,選取2010年7月至2011年9月252例福建地區無血緣關繫的漢族人為研究對象,納入的184例ACS患者再根據冠狀動脈(冠脈)造影結果分為單支病變組(60例)、雙支病變組(68例)、多支病變組(54例),其中2例造影冠脈無狹窄；以68例建康體檢者為對照.取靜脈血提取DNA,採用Sanger測序法檢測各組OPG基因209G/A、245T/G、1181G/C位點多態性.分析ACS組與對照組之間以及ACS不同病變程度組之間209G/A、245T/G、1181G/C各基因型和等位基因的分佈頻率以及基因多態性與ACS病變程度的關繫.結果 ACS組與對照組OPG 209G/A、245T/G位點基因型及等位基因分佈均無明顯差異；而1181G/C位點基因型分佈[GG型:92(50.0％)比43(63.2％),CC型:26(14.1％)比0,GC型:66(35.9％)比25 (36.8％),x2=11.240,P=0.004]、等位基因分佈[等位基因G:250(67.9％)比111(81.6％),等位基因C:118(32.1％)比25(18.4％),x2=9.148,P=0.002]差異均有統計學意義.進一步分析髮現,ACS單支病變組、雙支病變組及多支病變組209G/A、245T/G及1181G/C位點分佈比較均無明顯差異.結論 福建地區漢族人群OPG 209G/A、245T/G位點多態性與ACS無明顯相關性；1181G/C位點基因多態性與ACS髮病之間存在相關性,但與其病變程度無關.
목적 탐토복건지구한족인군골보호소(OPG)기인209G/A、245T/G、1181G/C위점기인다태성여급성관맥종합정(ACS)급기병변정도적상관성.방법 채용전첨성연구방법,선취2010년7월지2011년9월252례복건지구무혈연관계적한족인위연구대상,납입적184례ACS환자재근거관상동맥(관맥)조영결과분위단지병변조(60례)、쌍지병변조(68례)、다지병변조(54례),기중2례조영관맥무협착；이68례건강체검자위대조.취정맥혈제취DNA,채용Sanger측서법검측각조OPG기인209G/A、245T/G、1181G/C위점다태성.분석ACS조여대조조지간이급ACS불동병변정도조지간209G/A、245T/G、1181G/C각기인형화등위기인적분포빈솔이급기인다태성여ACS병변정도적관계.결과 ACS조여대조조OPG 209G/A、245T/G위점기인형급등위기인분포균무명현차이；이1181G/C위점기인형분포[GG형:92(50.0％)비43(63.2％),CC형:26(14.1％)비0,GC형:66(35.9％)비25 (36.8％),x2=11.240,P=0.004]、등위기인분포[등위기인G:250(67.9％)비111(81.6％),등위기인C:118(32.1％)비25(18.4％),x2=9.148,P=0.002]차이균유통계학의의.진일보분석발현,ACS단지병변조、쌍지병변조급다지병변조209G/A、245T/G급1181G/C위점분포비교균무명현차이.결론 복건지구한족인군OPG 209G/A、245T/G위점다태성여ACS무명현상관성；1181G/C위점기인다태성여ACS발병지간존재상관성,단여기병변정도무관.
Objective To explore the association of osteoprotegerin (OPG) gene polymorphisms 209G/A,245T/G and 1181G/C in the Han population of Fujian province with acute coronary syndrome (ACS) and its severity.Methods A prospective study was conducted.Two hundred and fifty-two cases without sibship of Fujian Han people was enrolled from July 2010 to September 2011,in which 68 healthy subjects as controls.One hundred and eighty-four patients with ACS were divided into three groups,60 had one-vessel,68 had two-vessel,and 54 had three-vessel lesion,2 had not been found stegnosis.DNA was extracted from venous blood,different groups of OPG gene polymorphism was identified by Sanger Method.OPG 209G/A,245T/G and 1181G/C genotype frequencies and allelic frequencies were analyzed between ACS subjects and normal subjects and among the three groups of ACS,and the relationship between gene polymorphisms and ACS severity.Results No significant differences were observed in the genotype and allelic frequencies of OPG gene 209G/A,245T/G between the ACS and control groups.Significant differences were observed in the genotype and allelic frequencies of OPG gene 1181G/C between the ACS and control groups [1181G/C GG:92 (50.0％) vs.43 (63.2％),CC:26 (14.1％) vs.0,GC:66 (35.9％) vs.25 (36.8％),x2=11.240,P=0.004; allelic gene G:250 (67.9％) vs.111 (81.6％),allelic gene C:118 (32.1％) vs.25 (18.4％),x2=9.148,P=0.002].No significant differences were observed in the genotype and allelic frequencies of OPG gene 209G/A,245T/G and 1181G/C between those groups.Conclusions There were no significant difference in frequencies OPG genotype and allele in polymorphisms 209G/A,245T/C between patients with ACS and control group in Han population of Fujian province.There were difference in frequencies OPG genotype and allele in polymorphisms 1181G/C between patients with ACS and control group,but it was not differently distributed among patients with single-,double-,or triple-vessel lesion.
