中国危重病急救医学
中國危重病急救醫學
중국위중병급구의학
CHINESE CRITICAL CARE MEDICINE
2012年
12期
750-753
,共4页
周晓峰%黄丁丁%王迪芬%付江泉
週曉峰%黃丁丁%王迪芬%付江泉
주효봉%황정정%왕적분%부강천
丙泊酚%预处理%谷氨酸%脑损伤%胶质纤维酸性蛋白
丙泊酚%預處理%穀氨痠%腦損傷%膠質纖維痠性蛋白
병박분%예처리%곡안산%뇌손상%효질섬유산성단백
Propofol%Pretreatment%Glutamate%Brain injury%Glial fibrillary acidic protein
目的 探讨丙泊酚预处理对谷氨酸(Glu)损伤大鼠脑组织的保护作用.方法 取出生10～ 15 dSD大鼠脑皮质切片进行培养,观察脑片形态学变化.将脑皮质切片分为空白对照组、Glu损伤组(1 mmol/L Glu 作用0.5 h)及丙泊酚预处理组(损伤前给予20 mg/L丙泊酚作用24 h),每组12个样本.镜下观察各组脑皮质切片的细胞病理改变及超微结构变化,计算乳酸脱氢酶(LDH)漏出率,免疫组化法检测胶质纤维酸性蛋白(GFAP)阳性表达并计数.结果 培养的脑皮质切片细胞形态完整、存活良好.苏木素-伊红(HE)染色、电镜及LDH检测结果显示:Glu损伤组脑皮质切片中神经元细胞损伤严重,形态不规则,胶质细胞增生、水肿,LDH漏出率较空白对照组明显增高[(68.5±2.0)％比(16.0±2.5)％,P＜0.01]；丙泊酚预处理组脑皮质切片神经元细胞损伤减轻,细胞形态恢复,LDH漏出率较Glu损伤组明显减少[(38.5±2.4)％比(68.5±2.0)％,P＜0.05].免疫组化检测结果显示:Glu损伤组胶质细胞胞体肿胀,突起数量增多,GFAP阳性反应强,阳性细胞数量(个/HP)较空白对照组显著增多(50±5比10±3,P＜0.01)；丙泊酚预处理组胶质细胞形态有所恢复,细胞突起细长,GFAP阳性反应减弱,阳性细胞数量较Glu损伤组明显减少(30±4比50±5,P＜0.05).结论 丙泊酚预处理对Glu损伤的SD大鼠脑皮质神经元具有保护作用.
目的 探討丙泊酚預處理對穀氨痠(Glu)損傷大鼠腦組織的保護作用.方法 取齣生10～ 15 dSD大鼠腦皮質切片進行培養,觀察腦片形態學變化.將腦皮質切片分為空白對照組、Glu損傷組(1 mmol/L Glu 作用0.5 h)及丙泊酚預處理組(損傷前給予20 mg/L丙泊酚作用24 h),每組12箇樣本.鏡下觀察各組腦皮質切片的細胞病理改變及超微結構變化,計算乳痠脫氫酶(LDH)漏齣率,免疫組化法檢測膠質纖維痠性蛋白(GFAP)暘性錶達併計數.結果 培養的腦皮質切片細胞形態完整、存活良好.囌木素-伊紅(HE)染色、電鏡及LDH檢測結果顯示:Glu損傷組腦皮質切片中神經元細胞損傷嚴重,形態不規則,膠質細胞增生、水腫,LDH漏齣率較空白對照組明顯增高[(68.5±2.0)％比(16.0±2.5)％,P＜0.01]；丙泊酚預處理組腦皮質切片神經元細胞損傷減輕,細胞形態恢複,LDH漏齣率較Glu損傷組明顯減少[(38.5±2.4)％比(68.5±2.0)％,P＜0.05].免疫組化檢測結果顯示:Glu損傷組膠質細胞胞體腫脹,突起數量增多,GFAP暘性反應彊,暘性細胞數量(箇/HP)較空白對照組顯著增多(50±5比10±3,P＜0.01)；丙泊酚預處理組膠質細胞形態有所恢複,細胞突起細長,GFAP暘性反應減弱,暘性細胞數量較Glu損傷組明顯減少(30±4比50±5,P＜0.05).結論 丙泊酚預處理對Glu損傷的SD大鼠腦皮質神經元具有保護作用.
목적 탐토병박분예처리대곡안산(Glu)손상대서뇌조직적보호작용.방법 취출생10～ 15 dSD대서뇌피질절편진행배양,관찰뇌편형태학변화.장뇌피질절편분위공백대조조、Glu손상조(1 mmol/L Glu 작용0.5 h)급병박분예처리조(손상전급여20 mg/L병박분작용24 h),매조12개양본.경하관찰각조뇌피질절편적세포병리개변급초미결구변화,계산유산탈경매(LDH)루출솔,면역조화법검측효질섬유산성단백(GFAP)양성표체병계수.결과 배양적뇌피질절편세포형태완정、존활량호.소목소-이홍(HE)염색、전경급LDH검측결과현시:Glu손상조뇌피질절편중신경원세포손상엄중,형태불규칙,효질세포증생、수종,LDH루출솔교공백대조조명현증고[(68.5±2.0)％비(16.0±2.5)％,P＜0.01]；병박분예처리조뇌피질절편신경원세포손상감경,세포형태회복,LDH루출솔교Glu손상조명현감소[(38.5±2.4)％비(68.5±2.0)％,P＜0.05].면역조화검측결과현시:Glu손상조효질세포포체종창,돌기수량증다,GFAP양성반응강,양성세포수량(개/HP)교공백대조조현저증다(50±5비10±3,P＜0.01)；병박분예처리조효질세포형태유소회복,세포돌기세장,GFAP양성반응감약,양성세포수량교Glu손상조명현감소(30±4비50±5,P＜0.05).결론 병박분예처리대Glu손상적SD대서뇌피질신경원구유보호작용.
Objective To study the protective effect of propofol precondition against glutamate (Glu) neurotoxicity to neonatal rat cerebrocortical slices.Methods Brain slices of Sprague-Dawley (SD) rats were cultured in vitro and observed the morpholgic changes.Brain slices were randomly divided into three groups:blank control group,Glu injury group (1 mmol/L Glu for 0.5 hour),propofol precondition group (20 mg/L propoful for 24 hours),each n=12.Changes in pathological and ultra-structure of cells were observed using microscope.Lactate dehydrogenase (LDH) leakage rate was measured.Meanwhile,the expression of glial fibrillary acidic protein (GFAP) was detected by immunohistochemical technology,then the positive cell were counted.Results Cultured brain slices of cell were intact and survived well.Hematoxylin-eosin (HE) staining,electron microscopy and LDH test results showed that cerebral film neuron severely damage,gliosis,edema,LDH leakage rate in Glu injury group were significantly more severe compared with blank control group [(68.5 ± 2.0)％ vs.(16.0 ± 2.5)％,P＜0.01].Reduce the brain slice of the propofol pretreatment group of neuronal cell jury,cell shape recovery significantly reduced LDH leakage rate compared with the Glu injury group [(38.5 ± 2.4)％ vs.(68.5 ± 2.0)％,P＜0.05].Immunohistochemical detection of GFAP expression of Glu injury group glial cell body swelling,producing increase in the number of GFAP positive reaction strong,the number of positive cells (cells/HP) compared with blank control group was significantly increased (50 ± 5 vs.10 ± 3,P＜0.01).The recovery of propofol pretreatment group glial cell morphology,cell processes slender GFAP positive reaction decreased the number of positive cells compared with the Glu injury group was significantly decreased (30 ± 4 vs.50 ± 5,P＜0.05).Conclusion Propofol pretreatment has prote ctive effect against Glu injuryed rat cerebrocortical slices.
