中华危重病急救医学
中華危重病急救醫學
중화위중병급구의학
Chinese Critical Care Medicine
2013年
10期
614-617
,共4页
石斌%马国光%刘鸿翔%刘景全%杨婉花%张宏泽%王晶%万晟霞
石斌%馬國光%劉鴻翔%劉景全%楊婉花%張宏澤%王晶%萬晟霞
석빈%마국광%류홍상%류경전%양완화%장굉택%왕정%만성하
脓毒症%红细胞%磷脂酰丝氨酸%贫血
膿毒癥%紅細胞%燐脂酰絲氨痠%貧血
농독증%홍세포%린지선사안산%빈혈
Sepsis%Erythrocytes%Phosphatidylserine%Anemia
目的 探讨红细胞膜表面磷脂酰丝氨酸(PS)外露在脓毒症患者贫血发生中的意义.方法 采用自身前后对照研究方法,选择30例重症监护病房(ICU)脓毒症患者,分别在入ICU 1 d、7d时采集外周静脉血,常规测定血红蛋白(Hb);采用膜联蛋白V/碘化丙啶(Annexin V/PI)染色方法,用流式细胞仪测定外周血中PS外露红细胞所占比例,分析Hb水平与PS外露红细胞比例之间的相关性.结果 30例脓毒症患者入ICU 7 d时的Hb水平较1d时显著下降(g/L:81.59±3.31比121.90±3.34,t=8.570,P=0.000),外周血中PS外露的红细胞比例也明显高于1d时[(17.19±1.35)％比(7.87±0.83)％,t=-6.557,P=0.000];Pearson直线相关分析显示,PS外露红细胞比例与Hb水平呈显著负相关(r=-0.838,P=0.000).结论 外周血PS外露的红细胞在脓毒症患者中明显增加,且与患者贫血程度有关,贫血程度越严重,红细胞PS外露水平越高.
目的 探討紅細胞膜錶麵燐脂酰絲氨痠(PS)外露在膿毒癥患者貧血髮生中的意義.方法 採用自身前後對照研究方法,選擇30例重癥鑑護病房(ICU)膿毒癥患者,分彆在入ICU 1 d、7d時採集外週靜脈血,常規測定血紅蛋白(Hb);採用膜聯蛋白V/碘化丙啶(Annexin V/PI)染色方法,用流式細胞儀測定外週血中PS外露紅細胞所佔比例,分析Hb水平與PS外露紅細胞比例之間的相關性.結果 30例膿毒癥患者入ICU 7 d時的Hb水平較1d時顯著下降(g/L:81.59±3.31比121.90±3.34,t=8.570,P=0.000),外週血中PS外露的紅細胞比例也明顯高于1d時[(17.19±1.35)％比(7.87±0.83)％,t=-6.557,P=0.000];Pearson直線相關分析顯示,PS外露紅細胞比例與Hb水平呈顯著負相關(r=-0.838,P=0.000).結論 外週血PS外露的紅細胞在膿毒癥患者中明顯增加,且與患者貧血程度有關,貧血程度越嚴重,紅細胞PS外露水平越高.
목적 탐토홍세포막표면린지선사안산(PS)외로재농독증환자빈혈발생중적의의.방법 채용자신전후대조연구방법,선택30례중증감호병방(ICU)농독증환자,분별재입ICU 1 d、7d시채집외주정맥혈,상규측정혈홍단백(Hb);채용막련단백V/전화병정(Annexin V/PI)염색방법,용류식세포의측정외주혈중PS외로홍세포소점비례,분석Hb수평여PS외로홍세포비례지간적상관성.결과 30례농독증환자입ICU 7 d시적Hb수평교1d시현저하강(g/L:81.59±3.31비121.90±3.34,t=8.570,P=0.000),외주혈중PS외로적홍세포비례야명현고우1d시[(17.19±1.35)％비(7.87±0.83)％,t=-6.557,P=0.000];Pearson직선상관분석현시,PS외로홍세포비례여Hb수평정현저부상관(r=-0.838,P=0.000).결론 외주혈PS외로적홍세포재농독증환자중명현증가,차여환자빈혈정도유관,빈혈정도월엄중,홍세포PS외로수평월고.
Objective To investigate the role of phosphatidylserine(PS)exposure of erythrocytes in the development of anemia in sepsis patients.Methods A self-control study was conducted.Thirty sepsis patients admitted to intensive care unit(ICU)were enrolled.Peripheral venous blood was collected on 1 day and 7 days after ICU admission,and hemoglobin(Hb)concentration was examined routinely.A flow-cytometric assay based on Annexin V/ propidium iodide(Annexin V/PI)was used to measure the PS exposure of erythrocytes.The relationship between PS exposure and Hb concentration was analyzed.Results Hb concentrations in 30 sepsis patients at 7 days after ICU admission were significantly decreased compared with those of patients at 1 day(g/L:81.59±3.31 vs.121.90±3.34,t=8.570,P=0.000),but the percentage of PS exposure of erythrocytes was significantly higher[(17.19 ±1.35)％ vs.(7.87±0.83)％,t=-6.557,P=0.000].An inverse correlation was found between percentage of PS-positive RBCs and Hb concentration by Pearson analysis(r=-0.838,P=0.000).Conclusions The percentage of PS exposure in erythrocytes is significantly increased in sepsis,and it might contribute to the development of anemia in sepsis patients during hospital stay.The more severe the anemia,the higher the PS in erythrocytes.
