中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2012年
11期
964-967
,共4页
付欢欢%余晓丹%余晓刚%任天虹%李娈娈%杨鑫
付歡歡%餘曉丹%餘曉剛%任天虹%李孌孌%楊鑫
부환환%여효단%여효강%임천홍%리련련%양흠
锰暴露%幼鼠%旷场试验%Morris水迷宫%学习记忆
錳暴露%幼鼠%曠場試驗%Morris水迷宮%學習記憶
맹폭로%유서%광장시험%Morris수미궁%학습기억
Mn exposure%Neonate rats%Open field%Morris water maze%Learning and memory
目的 研究不同剂量锰暴露对幼鼠空间学习记忆的影响.方法 刚出生的幼鼠随机分为对照组(生理盐水)和MnCl2 10,20,30 mg/kg组.每组1窝10只,给予腹腔注射生理盐水,自出生之日起,每日1次给予腹腔注射MnCl2溶液直至21d龄断奶.采用旷场实验观察幼鼠活动力；应用水迷宫检测大鼠空间学习及记忆能力.采用ICP-MS检测各组幼鼠的血锰以及海马组织锰含量.结果 1)对照组和10,20,30mg/kg组幼鼠的血锰水平分别为(35.58±13.77) μg/L,(80.00±12.98)μg/L,(238.51 ±31.43)μg/L,(348.47±34.07) μg/L；海马锰含量分别为576.82±79.78)μg/g,(798.33±40.60) μg/g,(1017.23±117.23) μg/g,(1278.76±281.48) μg/g].染锰组幼鼠血锰和海马锰含量比对照组均明显增高(P＜0.01),且海马锰含量与血锰水平呈正相关(OR=0.91,95％ CI=0.81～0.96,P＜0.01).2)旷场试验结果显示,各组活动的总距离差异无统计学意义(F=1.79,P＞0.05),表明锰暴露对幼鼠的运动活力没有影响；3)隐蔽平台实验中,仅在第3天,染锰组幼鼠到达隐蔽平台的时间比对照组显著延长(P＜0.01).对照组及10,20,30 mg/kg组幼鼠的逃避潜伏期分别为(21.77±7.10)s,(33.78±9.95)s,(37.17±13.68)s,(41.92±16.74)s,各染锰组间平均逃避潜伏期虽随着剂量的增加而延长,但差异无统计学意义.而其余训练天数各组幼鼠逃避潜伏期比较差异无统计学意义.探索实验结果显示,各组幼鼠的速度、穿台次数和在平台象限活动的时间均没有显著差异.结论 幼鼠锰暴露致使学习能力下降,但无明显的剂量效应关系；锰暴露对记忆能力没有影响.
目的 研究不同劑量錳暴露對幼鼠空間學習記憶的影響.方法 剛齣生的幼鼠隨機分為對照組(生理鹽水)和MnCl2 10,20,30 mg/kg組.每組1窩10隻,給予腹腔註射生理鹽水,自齣生之日起,每日1次給予腹腔註射MnCl2溶液直至21d齡斷奶.採用曠場實驗觀察幼鼠活動力；應用水迷宮檢測大鼠空間學習及記憶能力.採用ICP-MS檢測各組幼鼠的血錳以及海馬組織錳含量.結果 1)對照組和10,20,30mg/kg組幼鼠的血錳水平分彆為(35.58±13.77) μg/L,(80.00±12.98)μg/L,(238.51 ±31.43)μg/L,(348.47±34.07) μg/L；海馬錳含量分彆為576.82±79.78)μg/g,(798.33±40.60) μg/g,(1017.23±117.23) μg/g,(1278.76±281.48) μg/g].染錳組幼鼠血錳和海馬錳含量比對照組均明顯增高(P＜0.01),且海馬錳含量與血錳水平呈正相關(OR=0.91,95％ CI=0.81～0.96,P＜0.01).2)曠場試驗結果顯示,各組活動的總距離差異無統計學意義(F=1.79,P＞0.05),錶明錳暴露對幼鼠的運動活力沒有影響；3)隱蔽平檯實驗中,僅在第3天,染錳組幼鼠到達隱蔽平檯的時間比對照組顯著延長(P＜0.01).對照組及10,20,30 mg/kg組幼鼠的逃避潛伏期分彆為(21.77±7.10)s,(33.78±9.95)s,(37.17±13.68)s,(41.92±16.74)s,各染錳組間平均逃避潛伏期雖隨著劑量的增加而延長,但差異無統計學意義.而其餘訓練天數各組幼鼠逃避潛伏期比較差異無統計學意義.探索實驗結果顯示,各組幼鼠的速度、穿檯次數和在平檯象限活動的時間均沒有顯著差異.結論 幼鼠錳暴露緻使學習能力下降,但無明顯的劑量效應關繫；錳暴露對記憶能力沒有影響.
목적 연구불동제량맹폭로대유서공간학습기억적영향.방법 강출생적유서수궤분위대조조(생리염수)화MnCl2 10,20,30 mg/kg조.매조1와10지,급여복강주사생리염수,자출생지일기,매일1차급여복강주사MnCl2용액직지21d령단내.채용광장실험관찰유서활동력；응용수미궁검측대서공간학습급기억능력.채용ICP-MS검측각조유서적혈맹이급해마조직맹함량.결과 1)대조조화10,20,30mg/kg조유서적혈맹수평분별위(35.58±13.77) μg/L,(80.00±12.98)μg/L,(238.51 ±31.43)μg/L,(348.47±34.07) μg/L；해마맹함량분별위576.82±79.78)μg/g,(798.33±40.60) μg/g,(1017.23±117.23) μg/g,(1278.76±281.48) μg/g].염맹조유서혈맹화해마맹함량비대조조균명현증고(P＜0.01),차해마맹함량여혈맹수평정정상관(OR=0.91,95％ CI=0.81～0.96,P＜0.01).2)광장시험결과현시,각조활동적총거리차이무통계학의의(F=1.79,P＞0.05),표명맹폭로대유서적운동활력몰유영향；3)은폐평태실험중,부재제3천,염맹조유서도체은폐평태적시간비대조조현저연장(P＜0.01).대조조급10,20,30 mg/kg조유서적도피잠복기분별위(21.77±7.10)s,(33.78±9.95)s,(37.17±13.68)s,(41.92±16.74)s,각염맹조간평균도피잠복기수수착제량적증가이연장,단차이무통계학의의.이기여훈련천수각조유서도피잠복기비교차이무통계학의의.탐색실험결과현시,각조유서적속도、천태차수화재평태상한활동적시간균몰유현저차이.결론 유서맹폭로치사학습능력하강,단무명현적제량효응관계；맹폭로대기억능력몰유영향.
Objective To observe the effects of different levels manganese (Mn) on spatial learning and memory in neonate rats.Methods Neonate rats were distributed to control (normal saline) and MnCl210,20,30mg/kg groups randomly.Each groups included 10 litters in a cage with a dam.Neonate rats were intraperitoneal injection exposed to MnCl2 over PND 1-21.All groups were evaluated behavioral performance using open field and Morris water maze.Blood and hippocampus Mn levels were determined using ICP-MS.Results 1) For each group,blood Mn were (35.58 ± 13.77) μg/L,(80.00 ± 12.98) μg/L,(238.51 ± 31.43) μg/L,(348.47 ±34.07) μg/L and hippocampus Mn were (576.82 ± 79.78) μg/g,(798.33 ± 40.60) μg/g,(1017.23 ± 117.23)μg/g,(1278.76 ± 281.48) μg/g respectively.Blood and hippocampus Mn concentrations in Mn-exposed groups were significant increased compared to control (P ＜ 0.01),and there was a positive correlation in blood Mn and hippocampus Mn(OR =0.91,95％ CI=0.81-0.96,P＜ 0.01).2) Therewere no significant differences on travelled distance in open field among all groups,which meant that Mn exposure had no effect on their locomotion.3) In the hidden platform trials of the Morris water maze test,only on 3rd day,Mn-expose groups spent more time to find the platform compared to the control(P ＜ 0.01).The average escape latency were(21.77 ± 7.10)s,(33.78 ± 9.95)s,(37.17 ± 13.68) s,(41.92 ± 16.74) s respectively.Though the latency were increased with the Mn exposure levels increasing among the Mn-expose groups,no statistically significant differences were observed.There were no statistically effects on latency to find the platform of all groups in other training days.The result in probe trails showed that there were no statistically effects on swimming velocity,the number of crossing over the former platform and the time spent in the targeted quadrant.Conclusion Mn exposure exerts effects on the learning,but no doseeffect relationship.There are no effects on memory of neonate rats of Mn exposure.
