中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2012年
11期
1014-1016
,共3页
刘玉局%马海波%赵蓓%刘阳%袁勇贵
劉玉跼%馬海波%趙蓓%劉暘%袁勇貴
류옥국%마해파%조배%류양%원용귀
抑郁症%老年人%瘦素%瘦素受体基因%多态性%糖尿病
抑鬱癥%老年人%瘦素%瘦素受體基因%多態性%糖尿病
억욱증%노년인%수소%수소수체기인%다태성%당뇨병
Senile depressive patients%Leptin%Leptin receptor gene%Polymorphisms%Diabetes
目的 探讨瘦素受体基因3057G/A多态性对老年抑郁症患者血清瘦素(leptin)水平的影响.方法 采用放射免疫法对10例伴Ⅱ型糖尿病及47例不伴Ⅱ型糖尿病的老年抑郁症患者和35例正常老年人的血清瘦素浓度进行测定；采用聚合酶链式反应-限制性片段长度多态性技术,检测瘦素受体基因3057G/A多态性和等位基因分布频率.结果 (1)3组瘦素受体基因3057G/A多态性位点等位基因和基因型分布频率之间差异无显著性(P均＞0.05).(2)伴有和不伴有Ⅱ型糖尿病的老年抑郁症组血清瘦素浓度[(41.65 ±28.19) ng/ml,(25.56 ±21.04) ng/ml]显著高于正常老年人[(10.84 ±9.16) ng/ml](均P＜0.05)；伴有Ⅱ型糖尿病的老年抑郁症组血清瘦素水平又明显高于不伴有糖尿病的抑郁症组(P＜0.05).(3)携带AA型伴Ⅱ型糖尿病的老年抑郁症患者血清瘦素水平显著高于携带AA型的无糖尿病患者；携带AA型与AG/GG型的无糖尿病的老年抑郁症患者血清瘦素水平之间无显著差异(P＞0.05)；携带AA型与AG/GG型的对照组之间血清瘦素水平无明显差异(P＞0.05).结论 老年抑郁症存在瘦素分泌异常增高,伴Ⅱ型糖尿病患者更为明显；血清瘦素水平与Ⅱ型糖尿病之间存在关联,与瘦素受体基因3057G/A多态性无关联,Ⅱ型糖尿病与瘦素受体基因3057G/A多态性亦无关联性.
目的 探討瘦素受體基因3057G/A多態性對老年抑鬱癥患者血清瘦素(leptin)水平的影響.方法 採用放射免疫法對10例伴Ⅱ型糖尿病及47例不伴Ⅱ型糖尿病的老年抑鬱癥患者和35例正常老年人的血清瘦素濃度進行測定；採用聚閤酶鏈式反應-限製性片段長度多態性技術,檢測瘦素受體基因3057G/A多態性和等位基因分佈頻率.結果 (1)3組瘦素受體基因3057G/A多態性位點等位基因和基因型分佈頻率之間差異無顯著性(P均＞0.05).(2)伴有和不伴有Ⅱ型糖尿病的老年抑鬱癥組血清瘦素濃度[(41.65 ±28.19) ng/ml,(25.56 ±21.04) ng/ml]顯著高于正常老年人[(10.84 ±9.16) ng/ml](均P＜0.05)；伴有Ⅱ型糖尿病的老年抑鬱癥組血清瘦素水平又明顯高于不伴有糖尿病的抑鬱癥組(P＜0.05).(3)攜帶AA型伴Ⅱ型糖尿病的老年抑鬱癥患者血清瘦素水平顯著高于攜帶AA型的無糖尿病患者；攜帶AA型與AG/GG型的無糖尿病的老年抑鬱癥患者血清瘦素水平之間無顯著差異(P＞0.05)；攜帶AA型與AG/GG型的對照組之間血清瘦素水平無明顯差異(P＞0.05).結論 老年抑鬱癥存在瘦素分泌異常增高,伴Ⅱ型糖尿病患者更為明顯；血清瘦素水平與Ⅱ型糖尿病之間存在關聯,與瘦素受體基因3057G/A多態性無關聯,Ⅱ型糖尿病與瘦素受體基因3057G/A多態性亦無關聯性.
목적 탐토수소수체기인3057G/A다태성대노년억욱증환자혈청수소(leptin)수평적영향.방법 채용방사면역법대10례반Ⅱ형당뇨병급47례불반Ⅱ형당뇨병적노년억욱증환자화35례정상노년인적혈청수소농도진행측정；채용취합매련식반응-한제성편단장도다태성기술,검측수소수체기인3057G/A다태성화등위기인분포빈솔.결과 (1)3조수소수체기인3057G/A다태성위점등위기인화기인형분포빈솔지간차이무현저성(P균＞0.05).(2)반유화불반유Ⅱ형당뇨병적노년억욱증조혈청수소농도[(41.65 ±28.19) ng/ml,(25.56 ±21.04) ng/ml]현저고우정상노년인[(10.84 ±9.16) ng/ml](균P＜0.05)；반유Ⅱ형당뇨병적노년억욱증조혈청수소수평우명현고우불반유당뇨병적억욱증조(P＜0.05).(3)휴대AA형반Ⅱ형당뇨병적노년억욱증환자혈청수소수평현저고우휴대AA형적무당뇨병환자；휴대AA형여AG/GG형적무당뇨병적노년억욱증환자혈청수소수평지간무현저차이(P＞0.05)；휴대AA형여AG/GG형적대조조지간혈청수소수평무명현차이(P＞0.05).결론 노년억욱증존재수소분비이상증고,반Ⅱ형당뇨병환자경위명현；혈청수소수평여Ⅱ형당뇨병지간존재관련,여수소수체기인3057G/A다태성무관련,Ⅱ형당뇨병여수소수체기인3057G/A다태성역무관련성.
Objective To investigate the relation of serum leptin level in senile depressive patients with type 2 diabetes and polymorphisms of leptin receptor gene 3057 G/A.Methods 10 senile depressive patients with type 2 diabetes and 47 senile depressive patients without type 2 diabetes were selected for the study.Leptin levels were determined by radioimmunoassay.Genomitic DNA were obtained by phenol/chloroform method.Leptin receptor gene 3057 G/A polymorphisms and allele frequencies were determined with the polymerase chain reaction and restricted fragment length polymorphism assay.Results (1) The differences of leptin receptor genotypes and allele distribution in the 3 groups were not found (all P ＞ 0.05).(2) Serum leptin level in senile depressive patients with type 2 diabetes and without type 2 diabetic patients ((41.65 ± 28.19) ng/ml,(25.56 ± 21.04) ng/ml) were significantly higher than the normal people ((10.84 ± 9.16) ng/ml) (all P ＜ 0.05).Serum leptin level in senile depressive patients with type 2 diabetes were significantly higher than without type 2 diabetic patients(P＜ 0.05).(3) The serum leptin level in genotype AA senile depressive patients with type 2 diabetes were significantly higher than genotype AA patients without type 2 diabetes.The differences of the serum leptin levels in genotype AA senile depressive patients without type 2 diabetes and genotype AG/GG patients without type 2 diabetes were not found(P ＞ 0.05).The differences of the serum leptin level in genotype AA and genotype AG/GG in normal groups were not found (P＞ 0.05).Conclusion Senile depressive patients have higher serum leptin level.The serum leptin level is higher,especially with type 2 diabetes.Serum leptin level is related to type 2 diabetes.Serum leptin level is not related to the polymorphisms of leptin receptor gene 3057 G/A.Type 2 diabetes are not related to polymorphisms of leptin receptor gene 3057 G/A.
