中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2013年
4期
306-307
,共2页
悬尾实验%小鼠%品系%氟西汀
懸尾實驗%小鼠%品繫%氟西汀
현미실험%소서%품계%불서정
Tail suspension test%Mouse%Strain%Fluoxetine
目的 研究抗抑郁药氟西汀对小鼠悬尾实验的影响是否有品系差异性.方法 选择2个远交系(昆明和ICR)小鼠,3个近交系(C57BL/6,Balb/c,DBA/2)小鼠进行悬尾实验,给予选择性5-羟色胺再摄取抑制剂(SSRI)氟西汀,观察对悬尾不动时间的影响.结果 小鼠悬尾不动时间基础值有明显差异,其中时间最长的是C57BL/6小鼠[(145.0±16.8)s],而最短的是DBA/2小鼠[(34.5 ±6.1)s].氟西汀能显著降低C57BL/6[给药组(116.0 ±10.3)s,对照组(145.0±16.8)s]、Balb/c[给药组(44.3±6.2)s,对照组(75.3±10.3)s]和DBA/2小鼠[给药组(16.6±4.3)s,对照组(34.5±6.1)s]的悬尾不动时间(P＜0.05)；而昆明和ICR小鼠的不动时间在给予氟西汀后没有显著变化.结论 小鼠悬尾实验有明显的品系差异,氟西汀在C57BL/6、Balb/c和DBA/2小鼠上显示抗抑郁作用,而对昆明和ICR小鼠无明显抗抑郁效应.
目的 研究抗抑鬱藥氟西汀對小鼠懸尾實驗的影響是否有品繫差異性.方法 選擇2箇遠交繫(昆明和ICR)小鼠,3箇近交繫(C57BL/6,Balb/c,DBA/2)小鼠進行懸尾實驗,給予選擇性5-羥色胺再攝取抑製劑(SSRI)氟西汀,觀察對懸尾不動時間的影響.結果 小鼠懸尾不動時間基礎值有明顯差異,其中時間最長的是C57BL/6小鼠[(145.0±16.8)s],而最短的是DBA/2小鼠[(34.5 ±6.1)s].氟西汀能顯著降低C57BL/6[給藥組(116.0 ±10.3)s,對照組(145.0±16.8)s]、Balb/c[給藥組(44.3±6.2)s,對照組(75.3±10.3)s]和DBA/2小鼠[給藥組(16.6±4.3)s,對照組(34.5±6.1)s]的懸尾不動時間(P＜0.05)；而昆明和ICR小鼠的不動時間在給予氟西汀後沒有顯著變化.結論 小鼠懸尾實驗有明顯的品繫差異,氟西汀在C57BL/6、Balb/c和DBA/2小鼠上顯示抗抑鬱作用,而對昆明和ICR小鼠無明顯抗抑鬱效應.
목적 연구항억욱약불서정대소서현미실험적영향시부유품계차이성.방법 선택2개원교계(곤명화ICR)소서,3개근교계(C57BL/6,Balb/c,DBA/2)소서진행현미실험,급여선택성5-간색알재섭취억제제(SSRI)불서정,관찰대현미불동시간적영향.결과 소서현미불동시간기출치유명현차이,기중시간최장적시C57BL/6소서[(145.0±16.8)s],이최단적시DBA/2소서[(34.5 ±6.1)s].불서정능현저강저C57BL/6[급약조(116.0 ±10.3)s,대조조(145.0±16.8)s]、Balb/c[급약조(44.3±6.2)s,대조조(75.3±10.3)s]화DBA/2소서[급약조(16.6±4.3)s,대조조(34.5±6.1)s]적현미불동시간(P＜0.05)；이곤명화ICR소서적불동시간재급여불서정후몰유현저변화.결론 소서현미실험유명현적품계차이,불서정재C57BL/6、Balb/c화DBA/2소서상현시항억욱작용,이대곤명화ICR소서무명현항억욱효응.
Objective To study the strain difference in response to the antidepressant fluoxetine in mouse tail suspension test.Methods Two outbred mouse strains (KM and ICR) and three inbred mouse strains (C57BL/6,Balb/c and DBA/2) were used in this study.They were treated with the selective serotonin reuptake inhibitor (SSRI) fluoxetine or saline and the immobility time in tail suspension test was recorded.Results There was significant difference of baseline immobility time among different stains with C57BL/6 the most immobile((145.0 ±16.8) s) and DBA/2 the least ((34.5 ± 6.1)s).Fluoxetine significantly decreased the immobility time in C57BL/6 ((116.0 ± 10.3) s vs (145.0 ± 16.8) s) of control),Balb/c ((44.3 ± 6.2) s vs (75.3 ± 10.3) s) of control) and DBA/2 mice ((16.6 ± 4.3) s vs (34.5 ± 6.1) s) of control),while the immobile time of KM and ICR mice was not influenced by fluoxetine.Conclusion The effects of fluoxetine in tail suspension test are strain dependent.Fluoxetiue exhibits antidepressant effects in C57BL/6,Balb/c and DBA/2 mice,but not in KM and ICR mice.
