中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2013年
10期
877-879
,共3页
王永志%赵静洁%韩玉%杜仪%李宝金%李杨帆%刘路遥%张一帆%张银凤
王永誌%趙靜潔%韓玉%杜儀%李寶金%李楊帆%劉路遙%張一帆%張銀鳳
왕영지%조정길%한옥%두의%리보금%리양범%류로요%장일범%장은봉
利血平%抑郁模型%单胺类%神经递质
利血平%抑鬱模型%單胺類%神經遞質
리혈평%억욱모형%단알류%신경체질
Reserpine%Depression%Model%Monoamine neurotransmitter
目的 探索利血平致慢性抑郁大鼠模型的合适剂量,观察不同剂量利血平对大鼠自发活动和体质量的影响,分析模型大鼠海马单胺类神经递质的变化特点.方法 60只大鼠随机分为5组,空白组,利血平0.3 mg/kg、0.4 mg/kg、0.5 mg/kg、0.6 mg/kg组,每组n=12;动物造模给予腹腔注射利血平注射液1次/d(按分组中的给药剂量给予),空白组给予同体积蒸馏水,连续用药14 d.分别对大鼠open-field得分和体质量进行测量,酶联免疫法检测大鼠海马单胺类神经递质的水平.结果 慢性腹腔注射利血平(0.5、0.6 mg/kg)可引起大鼠体质量减轻(P<0.01,P<0.05),0.4、0.5、0.6 mg/kg组出现抑郁样行为;0.3 mg/kg组体质量、open-field得分变化不明显.与空白组体质量[(278.75±4.65)]和抑郁open-field评分[(60.3±19.9)分]比较,0.5 mg/kg组14 d大鼠体质量[(256.17±4.89)g]与open-field行为评分[(43.4±8.4)分]差异有显著性(P<0.01).0.5 mg/kg利血平对7d组抑郁大鼠海马5-羟色胺(5-HT)含量有影响[(2.83±1.09) ng/ml,P<0.05],对14 d组5-HT含量无明显影响.与空白组比较,0.5 mg/kg组14d腹腔注射利血平对单胺类神经递5-羟色胺(5-HT)、多巴胺(DA)、去甲肾上腺素(NA)影响差异无统计学意义(P>0.05),但可见5-HT与NA含量呈显著负相关(r=-0.449,P<0.01).结论 慢性腹腔注射0.5 mg/kg利血平能引起大鼠抑郁样行为,利血平对海马单胺类神经递质的影响与用药时间相关.
目的 探索利血平緻慢性抑鬱大鼠模型的閤適劑量,觀察不同劑量利血平對大鼠自髮活動和體質量的影響,分析模型大鼠海馬單胺類神經遞質的變化特點.方法 60隻大鼠隨機分為5組,空白組,利血平0.3 mg/kg、0.4 mg/kg、0.5 mg/kg、0.6 mg/kg組,每組n=12;動物造模給予腹腔註射利血平註射液1次/d(按分組中的給藥劑量給予),空白組給予同體積蒸餾水,連續用藥14 d.分彆對大鼠open-field得分和體質量進行測量,酶聯免疫法檢測大鼠海馬單胺類神經遞質的水平.結果 慢性腹腔註射利血平(0.5、0.6 mg/kg)可引起大鼠體質量減輕(P<0.01,P<0.05),0.4、0.5、0.6 mg/kg組齣現抑鬱樣行為;0.3 mg/kg組體質量、open-field得分變化不明顯.與空白組體質量[(278.75±4.65)]和抑鬱open-field評分[(60.3±19.9)分]比較,0.5 mg/kg組14 d大鼠體質量[(256.17±4.89)g]與open-field行為評分[(43.4±8.4)分]差異有顯著性(P<0.01).0.5 mg/kg利血平對7d組抑鬱大鼠海馬5-羥色胺(5-HT)含量有影響[(2.83±1.09) ng/ml,P<0.05],對14 d組5-HT含量無明顯影響.與空白組比較,0.5 mg/kg組14d腹腔註射利血平對單胺類神經遞5-羥色胺(5-HT)、多巴胺(DA)、去甲腎上腺素(NA)影響差異無統計學意義(P>0.05),但可見5-HT與NA含量呈顯著負相關(r=-0.449,P<0.01).結論 慢性腹腔註射0.5 mg/kg利血平能引起大鼠抑鬱樣行為,利血平對海馬單胺類神經遞質的影響與用藥時間相關.
목적 탐색리혈평치만성억욱대서모형적합괄제량,관찰불동제량리혈평대대서자발활동화체질량적영향,분석모형대서해마단알류신경체질적변화특점.방법 60지대서수궤분위5조,공백조,리혈평0.3 mg/kg、0.4 mg/kg、0.5 mg/kg、0.6 mg/kg조,매조n=12;동물조모급여복강주사리혈평주사액1차/d(안분조중적급약제량급여),공백조급여동체적증류수,련속용약14 d.분별대대서open-field득분화체질량진행측량,매련면역법검측대서해마단알류신경체질적수평.결과 만성복강주사리혈평(0.5、0.6 mg/kg)가인기대서체질량감경(P<0.01,P<0.05),0.4、0.5、0.6 mg/kg조출현억욱양행위;0.3 mg/kg조체질량、open-field득분변화불명현.여공백조체질량[(278.75±4.65)]화억욱open-field평분[(60.3±19.9)분]비교,0.5 mg/kg조14 d대서체질량[(256.17±4.89)g]여open-field행위평분[(43.4±8.4)분]차이유현저성(P<0.01).0.5 mg/kg리혈평대7d조억욱대서해마5-간색알(5-HT)함량유영향[(2.83±1.09) ng/ml,P<0.05],대14 d조5-HT함량무명현영향.여공백조비교,0.5 mg/kg조14d복강주사리혈평대단알류신경체5-간색알(5-HT)、다파알(DA)、거갑신상선소(NA)영향차이무통계학의의(P>0.05),단가견5-HT여NA함량정현저부상관(r=-0.449,P<0.01).결론 만성복강주사0.5 mg/kg리혈평능인기대서억욱양행위,리혈평대해마단알류신경체질적영향여용약시간상관.
Objective To observe the suitable dosage of reserpine for the establishment of rat model of depression and analyze the changes of monoamine neurotransmitter in hippocampus.Methods 60 rats were randomly divided into five groups:the normal group,the 0.3 mg/kg group,0.4 mg/kg group,0.5 mg/kg group and 0.6 mg/kg group,and 12 rats in each group.The model groups were intraperitoneally administrated by different dosage of reserpine (according to the dosage of the grouping) for 14 days and rats in the normal group were injected the same volume of solvent.The body weight was recorded at different days and activity was tested by openfield test at 0,7th and 14th day.Monoamine neurotransmitter changes in hippocampus were tested by enzymelinked immunosorbent assay.Results Reserpine could cause body weight loss (0.5,0.6 mg/kg) and depressive behavior (0.4,0.5,0.6 mg/kg).The group of 0.4 mg/kg showed no difference in weight and open-field test.Compared with the weight body ((278.75 ± 4.65) g) and open-field test (60.3 ± 19.9) at the model group,the group of 0.5 mg/kg at 14th showed significant differences in weight ((256.17 ±4.89) g) and behavior(43.4 ±8.4) (P<0.01).Dosage of 0.5 mg/kg had effect to 5-HT in hippocampus at 7th day ((2.83 ± 1.09) ng/ml,P<0.01) but not 14th day.Compare with the normal group at 14th day,the group of 0.5 mg/kg showed no difference at 5-HT,DA and NA,and there was significant negative correlation between 5-HT and NA(r=-0.449,P<0.01).Conclusion Reserpine of 5 mg/kg can result in the depressive behavior of rats.The effect of reserpine to monoamine neurotransmitter in hippocampus has relationship with time.