CAJ | 학술논문

目的探讨芒果苷(Mangiterin)对慢性应激抑郁模型(chronic unpredictable stress,CUS)小鼠行为学的改善作用及对海马脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)表达的影响.方法将大鼠随机分为正常对照组,慢性应激模型组,氟西汀组,芒果苷低、中和高剂量组,共6组,每组10只.采用慢性轻度不可预见性应激配合孤养复制抑郁模型,通过旷场及悬尾实验观察行为学改变,并运用免疫印迹检测海马BDNF的表达.结果与正常对照组比,模型组体质量增长缓慢[(5.33±1.20)g],水平穿格次数减少[(102±18)次/5 min],活动距离缩短[(3425 ± 112) mm],悬尾不动时间延长,行为学明显处于抑郁状态,同时降低海马BDNF的表达(0.45±0.03),差异有统计学意义(P＜0.01)；而中药芒果苷组较模型组可显著改善行为学指标,体质量增长较快,升高海马BDNF的表达,差异有统计学意义(P＜0.01).芒果苷高剂量组与氟西汀组比较,差异无统计学意义(P＞0.05).结论芒果苷能够改善慢性应激抑郁模型小鼠的抑郁行为,可能与其上调海马BDNF的表达有关.
목적 탐토망과감(Mangiterin)대만성응격억욱모형(chronic unpredictable stress,CUS)소서행위학적개선작용급대해마뇌원성신경영양인자(brain-derived neurotrophic factor,BDNF)표체적영향.방법 장대서수궤분위정상대조조,만성응격모형조,불서정조,망과감저、중화고제량조,공6조,매조10지.채용만성경도불가예견성응격배합고양복제억욱모형,통과광장급현미실험관찰행위학개변,병운용면역인적검측해마BDNF적표체.결과 여정상대조조비,모형조체질량증장완만[(5.33±1.20)g],수평천격차수감소[(102±18)차/5 min],활동거리축단[(3425 ± 112) mm],현미불동시간연장,행위학명현처우억욱상태,동시강저해마BDNF적표체(0.45±0.03),차이유통계학의의(P＜0.01)；이중약망과감조교모형조가현저개선행위학지표,체질량증장교쾌,승고해마BDNF적표체,차이유통계학의의(P＜0.01).망과감고제량조여불서정조비교,차이무통계학의의(P＞0.05).결론 망과감능구개선만성응격억욱모형소서적억욱행위,가능여기상조해마BDNF적표체유관.
Objective To explore the effects of mangiterin on behaviors and brain-derived neurotrophic factor(BDNF) of hippocampus in chronic stress depression mice.Methods 60 male mice were randomly divided into normol control group,model group,fluoxetin control group and mangiterin groups (low,medium and high dose),10 mice in each group.All mice except normal control group were singly housed and subjected to chronic stress-induced depression model for 21 consecutive days.The behaviors of mice were detected by open-field test and tail-suspension test.The expression of BDNF in the hippocampus were assessed using western blot.Results Compared with the normal group,mice exposed chronic stress showed decreased body weight((5.33 ±1.20) g),crossing lines (102 ± 18) and distances ((3425 ± 112) mm) notably decreased in open-field behavior test.The duration of immobility during tail-suspension was increased significantly.BDNF expression in the hippocampus(0.45 ± 0.03) was downregulated significantly(P＜ 0.01),while it was upregulated in the groups of mangiterin(P ＜ 0.01).Mangiterin group in high dose had the similar effects to fluoxetin group(P ＞ 0.05).Conclusion Mangiterin can ameliorate behavior impairment of chronic stress induced-depression mice and it may be related to the upregulation of BDNF expression in the hippocampus.