CAJ | 학술논문

目的探讨红藻氨酸(kainic acid,KA)致痫大鼠外周血炎性因子的水平与癫痫严重程度的关系.方法 140只Wistar大鼠随机分为对照组和模型组,每组70只.模型组以10 mg/kg KA腹腔注射建立模型,对照组以生理盐水同样处理.观察行为学变化.以ELISA方法检测不同时点血清TNF-α、IL-1β、IL-4和IL-10的表达水平.结果 KA注射后,大鼠在3h ～ 9h进入癫痫大发作期.与对照组相比[6 h:(12.3±3.1) pg/ml,12 h:(11.5±3.8) pg/ml],模型组在6h～12h促炎因子TNF-α[6 h:(21.5±3.2) pg/ml,12h:(20.6±4.2) pg/ml]和IL-1β的水平明显升高(P＜0.05);抗炎因子IL-4只在12 h时明显升高[(53.55±3.08) pg/ml,(33.26±4.16) pg/ml] (P＜0.05),IL-10水平差异无统计学意义(P＞0.05).结论促炎因子TNF-α和IL-1β参与了癫痫的发作过程,其水平与癫痫发作程度密切相关;抗炎因子IL-4和IL-10水平的变化与癫痫的发作程度无显著相关性.
목적 탐토홍조안산(kainic acid,KA)치간대서외주혈염성인자적수평여전간엄중정도적관계.방법 140지Wistar대서수궤분위대조조화모형조,매조70지.모형조이10 mg/kg KA복강주사건립모형,대조조이생리염수동양처리.관찰행위학변화.이ELISA방법검측불동시점혈청TNF-α、IL-1β、IL-4화IL-10적표체수평.결과 KA주사후,대서재3h ～ 9h진입전간대발작기.여대조조상비[6 h:(12.3±3.1) pg/ml,12 h:(11.5±3.8) pg/ml],모형조재6h～12h촉염인자TNF-α[6 h:(21.5±3.2) pg/ml,12h:(20.6±4.2) pg/ml]화IL-1β적수평명현승고(P＜0.05);항염인자IL-4지재12 h시명현승고[(53.55±3.08) pg/ml,(33.26±4.16) pg/ml] (P＜0.05),IL-10수평차이무통계학의의(P＞0.05).결론 촉염인자TNF-α화IL-1β삼여료전간적발작과정,기수평여전간발작정도밀절상관;항염인자IL-4화IL-10수평적변화여전간적발작정도무현저상관성.
Objectives To investigate the correlation between the level of peripheral blood inflammatory cytokines and kainic acid-induced seizure severity in rats.Methods 140 rats were divided into control and model group randomly,70 rats in each group.Model group rats were injected with kainic acid (10 mg/kg) by intraperitoneal,and the control rats were injected with sodium chloride.The change of their behaviors was observed and the concentrations of TNF-α,IL-1β,IL-4 and IL-10 were determined by ELISA in each group at different times.Results The rats showed epilepsy grand mal in 3 h-9 h after KA injection.The concentrations of TNF-α and IL-1β in model groups were significantly higher than those in control group in 6 h-12 h (6 h:(21.5±3.2) pg/ml vs (12.3±3.1)pg/ml;12 h:(20.6±4.2)pg/ml vs (11.5±3.8)pg/ml)(P＜0.05) and IL-4 in model group was higher at only 12 h ((53.55±3.08) pg/ml vs (33.26±4.16)pg/ml) (P＜0.05).The level of IL-10 in model groups was not statistically significant compared with control group (P＞0.05).Conclusion The proinflammatory cytokines (TNF-α and IL-l β) participate in the seizure procedure,meanwhile their levels and seizure severity have eminent correlations,but antiinflammatory cytokines (IL-4 and IL-10) had not.