CAJ | 학술논문

目的探讨鞘内注射丹参酮ⅡA对骨癌痛小鼠热痛觉过敏及脊髓水平白介素-1β(IL-1β)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)表达的影响.方法 C3H/HeNCrlVr雄性小鼠84只,应用随机数字表法分为:(1)丹参酮ⅡA 10μg组;(2)丹参酮ⅡA 20μg组;(3)丹参酮ⅡA 40μg组;(4)正常组;(5)DMSO+Sham组;(6)丹参酮ⅡA+ Sham组;(7)DMSO+ Tumor组.用热辐射刺激仪测定缩足潜伏期(PWTL).应用Real-time PCR技术检测mRNA表达.结果各组小鼠基础PWTL差异无统计学意义(P＞0.05).术后14d,与正常组相比,假手术组PWTL差异无统计学意义(P＞0.05),而骨癌痛组PWTL明显降低(P＜0.05).术后21d,和正常组相比,丹参酮ⅡA +Sham组、DMSO+ Sham组PWTL和脊髓IL-1β、IL-6、TNF-α表达差异均无统计学意义(P＞0.05);DMSO+Tumor组PWTL[(6.19±1.26)s]明显低于正常组[(16.01±1.59)s](P＜0.05),脊髓IL-1β、IL-6、TNF-α表达则高于正常组;丹参酮ⅡA 20μg组[(9.83±1.26)s]、丹参酮ⅡA 40 μg组[(10.29±2.95)s]PWTL高于DMSO+Tumor组,脊髓IL-1β、IL-6、TNF-α表达则低于DMSO+Tumor组(P＜0.05);丹参酮ⅡA 10 μg组PWTL[(6.67±0.96)s]、脊髓IL-1β、IL-6、TNF-α表达水平与DMSO+Tumor组相比差异无统计学意义(P＞0.05).结论鞘内注射丹参酮ⅡA具有剂量依赖性抗癌痛作用,抑制脊髓水平表达释放炎症因子IL-1β、IL-6、TNF-α可能是其机制之一.
목적 탐토초내주사단삼동ⅡA대골암통소서열통각과민급척수수평백개소-1β(IL-1β)、백개소-6(IL-6)、종류배사인자-α(TNF-α)표체적영향.방법 C3H/HeNCrlVr웅성소서84지,응용수궤수자표법분위:(1)단삼동ⅡA 10μg조;(2)단삼동ⅡA 20μg조;(3)단삼동ⅡA 40μg조;(4)정상조;(5)DMSO+Sham조;(6)단삼동ⅡA+ Sham조;(7)DMSO+ Tumor조.용열복사자격의측정축족잠복기(PWTL).응용Real-time PCR기술검측mRNA표체.결과 각조소서기출PWTL차이무통계학의의(P＞0.05).술후14d,여정상조상비,가수술조PWTL차이무통계학의의(P＞0.05),이골암통조PWTL명현강저(P＜0.05).술후21d,화정상조상비,단삼동ⅡA +Sham조、DMSO+ Sham조PWTL화척수IL-1β、IL-6、TNF-α표체차이균무통계학의의(P＞0.05);DMSO+Tumor조PWTL[(6.19±1.26)s]명현저우정상조[(16.01±1.59)s](P＜0.05),척수IL-1β、IL-6、TNF-α표체칙고우정상조;단삼동ⅡA 20μg조[(9.83±1.26)s]、단삼동ⅡA 40 μg조[(10.29±2.95)s]PWTL고우DMSO+Tumor조,척수IL-1β、IL-6、TNF-α표체칙저우DMSO+Tumor조(P＜0.05);단삼동ⅡA 10 μg조PWTL[(6.67±0.96)s]、척수IL-1β、IL-6、TNF-α표체수평여DMSO+Tumor조상비차이무통계학의의(P＞0.05).결론 초내주사단삼동ⅡA구유제량의뢰성항암통작용,억제척수수평표체석방염증인자IL-1β、IL-6、TNF-α가능시기궤제지일.
Objective To investigate the effect of intrathecal injection of Tanshinone Ⅱ A on bone cancer pain behavior and spinal IL-1 β,IL-6,TNF-α expression.Methods According to the random number table method,84 C3H/HeNCrlVr male mice were divided into:(1) Tanshinone ⅡA 10 μg group:the tumor mice were treated by intrathecal administration (once daily on the days 14-20 after inoculation of tumor cells) with Tanshinone ⅡA 10 μg; (2)Tanshinone ⅡA 20 μg group:the tumor mice were treated with Tanshinone ⅡA 20 μg; (3)Tanshinone ⅡA 40 μg group:the tumor mice were treated with Tanshinone ⅡA 40 μg; (4) normal control group:the mice were given food and water ad libitum; (5) DMSO+Sham group:the sham mice were treated with the same volume of 5％DMSO; (6) Tanshinone ⅡA+Sham group:the sham mice were treated with Tanshinone ⅡA 40 μg; (7)DMSO+Tumor group:the tumor mice were treated with the same volume of 5％DMSO.The mice pain behaviors were assessed with the paw withdrawal thermal latency (PWTL) at the corresponding time points,then the mice were killed and the samples of spinal cord were detected by real-time PCR.Results The basic values of PWTL had no significant differences among all groups (P＞0.05).At day 14 after operation,no significant difference (P＞0.05) was found in the PWTL value between normal control group and the sham operation group.But in tumor group,the PWTL value was significantly lower than that of normal control group (P＜0.05).At day 21 after operation,the PWTL and the level of spinal IL-1 β,IL-6,TNF-α expression had no significant differences (P＞0.05) among normal control group,Tanshinone ⅡA+Sham group and DMSO+Sham group.The PWTL ((6.19± 1.26)s) in DMSO+ Tumor group was significantly lower than that of normal control group((16.01± 1.59)s) (P＜0.05),but the level of IL-1β,IL-6,TNF-α expression was higher than that of normal control group.Compared with the normal group,the PWTL ((9.83±1.26)s;(10.29±2.95) s) of Tanshinone Ⅱ A 20 μg and 40 μg group was higher,and the level of spinal IL-1β,IL-6,TNF-α expression was lower (P＜0.05).The PWTL and the levels of spinal IL-1β,IL-6,TNF-αexpression had no significant differences between Tanshinone ⅡA 10 μg group ((6.67 ± 0.96) s) and DMSO + Tumor group(P＞0.05).Conclusion Intrathecal injection of Tanshinone Ⅱ A plays a role in anti-cancer pain,and inhibition of spinal inflammatory cytokine release may be one of its mechanisms.