中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2013年
11期
982-984
,共3页
戴伟娟%王国芳%林丽文%辛勤
戴偉娟%王國芳%林麗文%辛勤
대위연%왕국방%림려문%신근
温脾汤%脑缺血再灌注%记忆%超氧化物歧化酶%丙二醛
溫脾湯%腦缺血再灌註%記憶%超氧化物歧化酶%丙二醛
온비탕%뇌결혈재관주%기억%초양화물기화매%병이철
Wen-pi-tang%Cerebral ischemia%Reperfusion%Memory%SOD%MDA
目的 探讨温脾汤提取物对正常和脑缺血再灌小鼠记忆功能及脑内超氧化物歧化酶(SOD)及丙二醛(MDA)的影响.方法 将小鼠分为正常组、假手术组、模型组、温脾汤水提物和醇提物各三个剂量组(1.0 g/kg、0.5 g/kg、0.25 g/kg).采用反复结扎双侧颈总动脉制备小鼠脑缺血再灌损伤模型.利用跳台和避暗实验,测试温脾汤提取物连续灌胃10d,对正常和脑缺血再灌小鼠记忆行为的影响,并检测小鼠脑内SOD活性和MDA含量.结果 跳台实验中,与生理盐水组相比,温脾汤水提物和醇提物各剂量导致小鼠的潜伏期延长、错误次数减少,以高剂量最明显(P＜0.05)[潜伏期:(187.17±99.00)s,(270.73±44.73)s,(260.45±63.78)s;错误次数(1.75±1.71)次,(0.45±0.69)次,(0.75±0.97)次];避暗实验中,与模型组相比,水提物高、中剂量和醇提物高剂量能使脑缺血再灌小鼠的潜伏期延长及错误次数减少(P＜0.05)[潜伏期(157.15±84.91)s,(250.63±58.98)s,(251.12±78.22)s,(245±84.82)s;错误次数(2.15±1.22)次,(0.75±0.89)次,(0.75±1.16)次,(0.625±0.92)次];温脾汤水提物高、中剂量小鼠脑内SOD活性较模型组明显增强((P＜0.05),MDA的含量与模型组相比明显降低(P＜0.05,P＜0.01).结论 温脾汤提取物可提高正常小鼠的记忆功能,改善脑缺血再灌小鼠记忆功能,其机制可能与恢复脑组织SOD活力及减少MDA含量有关.
目的 探討溫脾湯提取物對正常和腦缺血再灌小鼠記憶功能及腦內超氧化物歧化酶(SOD)及丙二醛(MDA)的影響.方法 將小鼠分為正常組、假手術組、模型組、溫脾湯水提物和醇提物各三箇劑量組(1.0 g/kg、0.5 g/kg、0.25 g/kg).採用反複結扎雙側頸總動脈製備小鼠腦缺血再灌損傷模型.利用跳檯和避暗實驗,測試溫脾湯提取物連續灌胃10d,對正常和腦缺血再灌小鼠記憶行為的影響,併檢測小鼠腦內SOD活性和MDA含量.結果 跳檯實驗中,與生理鹽水組相比,溫脾湯水提物和醇提物各劑量導緻小鼠的潛伏期延長、錯誤次數減少,以高劑量最明顯(P＜0.05)[潛伏期:(187.17±99.00)s,(270.73±44.73)s,(260.45±63.78)s;錯誤次數(1.75±1.71)次,(0.45±0.69)次,(0.75±0.97)次];避暗實驗中,與模型組相比,水提物高、中劑量和醇提物高劑量能使腦缺血再灌小鼠的潛伏期延長及錯誤次數減少(P＜0.05)[潛伏期(157.15±84.91)s,(250.63±58.98)s,(251.12±78.22)s,(245±84.82)s;錯誤次數(2.15±1.22)次,(0.75±0.89)次,(0.75±1.16)次,(0.625±0.92)次];溫脾湯水提物高、中劑量小鼠腦內SOD活性較模型組明顯增彊((P＜0.05),MDA的含量與模型組相比明顯降低(P＜0.05,P＜0.01).結論 溫脾湯提取物可提高正常小鼠的記憶功能,改善腦缺血再灌小鼠記憶功能,其機製可能與恢複腦組織SOD活力及減少MDA含量有關.
목적 탐토온비탕제취물대정상화뇌결혈재관소서기억공능급뇌내초양화물기화매(SOD)급병이철(MDA)적영향.방법 장소서분위정상조、가수술조、모형조、온비탕수제물화순제물각삼개제량조(1.0 g/kg、0.5 g/kg、0.25 g/kg).채용반복결찰쌍측경총동맥제비소서뇌결혈재관손상모형.이용도태화피암실험,측시온비탕제취물련속관위10d,대정상화뇌결혈재관소서기억행위적영향,병검측소서뇌내SOD활성화MDA함량.결과 도태실험중,여생리염수조상비,온비탕수제물화순제물각제량도치소서적잠복기연장、착오차수감소,이고제량최명현(P＜0.05)[잠복기:(187.17±99.00)s,(270.73±44.73)s,(260.45±63.78)s;착오차수(1.75±1.71)차,(0.45±0.69)차,(0.75±0.97)차];피암실험중,여모형조상비,수제물고、중제량화순제물고제량능사뇌결혈재관소서적잠복기연장급착오차수감소(P＜0.05)[잠복기(157.15±84.91)s,(250.63±58.98)s,(251.12±78.22)s,(245±84.82)s;착오차수(2.15±1.22)차,(0.75±0.89)차,(0.75±1.16)차,(0.625±0.92)차];온비탕수제물고、중제량소서뇌내SOD활성교모형조명현증강((P＜0.05),MDA적함량여모형조상비명현강저(P＜0.05,P＜0.01).결론 온비탕제취물가제고정상소서적기억공능,개선뇌결혈재관소서기억공능,기궤제가능여회복뇌조직SOD활력급감소MDA함량유관.
Objective To explore the influence of the extract of wen-pi-tang on the memory and SOD and MDA of normal mice and cerebral ischemia reperfusion mice.Method The mice were divided into normal group,sham operation group,model group,aqueous extract and alcohol extract of wen-pi-tang (1.0 g/kg,0.5 g/kg,0.25g/kg).Model mice with cerebral ischemia reperfusion were made by blocking bilateral common carotid artery.The influence on memory behavior of normal mice and mice with cerebral ischemia reperfusion were tested by gavage for 10 days with extract of wen-pi-tang through step-down and step-through tests.Then SOD activity and content of the MDA were tested.Result In step-down test,compared with normal group,extract of wen-pi-tang with all doses made normal mice' latency longer,error frequency fewer,with high does clearest (P＜0.05)(latency:(187.17±99.00) s,(270.73±44.73) s,(260.45±63.78) s,error frequency(1.75± 1.71) times,(0.45±0.69) times,(0.75±0.97) times) ;in step-through test,compared with normal group,aqueous extract with high and middle doses and alcohol extract with longe doses could made latency of cerebral ischemia reperfusion mice longer and error frequency fewer (latency(157.15±84.91) s,(250.63±58.98) s,(251.12±78.22) s,(245±84.82) s,error frequency(2.15± 1.22) times,(0.75±0.89) times,(0.75± 1.16) times,(0.625 ±0.92) times) (P＜0.05) ; extract of wen-pi-tang with high and middle does increased SOD activity obviously compared with normal group(P＜0.05),and the content of MDA was obviously lower compared with normal group(P＜0.05,P＜0.01).Conclusion Extracts of wen-pi-tang can improve the memory function of normal mice,and the memory of cerebral ischemia reperfusion mice.The mechanism can be related to the increase of SOD activity and the decrease of the content of MDA.
