CAJ | 학술논문

目的观察静脉移植骨髓单个核细胞(bone marrow mononuclear cells,BMMNCs)治疗脑出血(ICH)大鼠脑损伤的效果及对出血后炎症反应的影响.方法应用立体定位技术将Ⅳ型胶原酶注入大鼠尾状核纹状体内制作脑出血模型,建模后按随机化原则分为假手术组、ICH组、PBS组、BMMNCs移植组；梯度离心法提取、分离、纯化大鼠骨髓单个核细胞,ICH 6 h后经股静脉输注3× 107BMMNCs或等量PBS缓冲液.采用改良神经功能损伤评分评价脑出血后1d、3d、7d、14 d神经行为功能改善情况；干湿重法测定血肿周围脑组织含水量；免疫荧光法检测脑出血侧小胶质细胞活化及中性粒细胞浸润情况.结果 BMMNCs移植组大鼠的神经缺损症状在出血后7d、14d明显改善,与ICH组及PBS组比较,差异有统计学意义(P＜0.05)；在脑出血后3d,BMMNCs移植组的大鼠脑组织含水量[(78.62±0.97)％]明显降低,与ICH组[(81.09±0.83)％]及PBS组[(80.99±0.79)％]比较,差异有统计学意义(P＜0.05)；BMMNCs移植组脑内小胶质细胞活化增殖及中性粒细胞浸润数目[(55.8±22.1)个/mm2、(49.6±12.9)个/mm2]均显著低于PBS组[(125.0±20.7)个/mm2、(86.8±13.6)个/mm2],差异有统计学意义(P＜0.01).结论骨髓单个核细胞可明显减轻脑出血后脑水肿程度,促进神经功能恢复,其机制与其减轻脑出血后小胶质细胞活化和中性粒细胞浸润有关.
목적 관찰정맥이식골수단개핵세포(bone marrow mononuclear cells,BMMNCs)치료뇌출혈(ICH)대서뇌손상적효과급대출혈후염증반응적영향.방법 응용입체정위기술장Ⅳ형효원매주입대서미상핵문상체내제작뇌출혈모형,건모후안수궤화원칙분위가수술조、ICH조、PBS조、BMMNCs이식조；제도리심법제취、분리、순화대서골수단개핵세포,ICH 6 h후경고정맥수주3× 107BMMNCs혹등량PBS완충액.채용개량신경공능손상평분평개뇌출혈후1d、3d、7d、14 d신경행위공능개선정황；간습중법측정혈종주위뇌조직함수량；면역형광법검측뇌출혈측소효질세포활화급중성립세포침윤정황.결과 BMMNCs이식조대서적신경결손증상재출혈후7d、14d명현개선,여ICH조급PBS조비교,차이유통계학의의(P＜0.05)；재뇌출혈후3d,BMMNCs이식조적대서뇌조직함수량[(78.62±0.97)％]명현강저,여ICH조[(81.09±0.83)％]급PBS조[(80.99±0.79)％]비교,차이유통계학의의(P＜0.05)；BMMNCs이식조뇌내소효질세포활화증식급중성립세포침윤수목[(55.8±22.1)개/mm2、(49.6±12.9)개/mm2]균현저저우PBS조[(125.0±20.7)개/mm2、(86.8±13.6)개/mm2],차이유통계학의의(P＜0.01).결론 골수단개핵세포가명현감경뇌출혈후뇌수종정도,촉진신경공능회복,기궤제여기감경뇌출혈후소효질세포활화화중성립세포침윤유관.
Objective To investigate the effect of intravenously transplanted bone marrow mononuclear cells (BMMNCs) on brain injury and inflammation after intracerebral hemorrhage(ICH) in rats.Methods Experimnental ICH models were performed by stereotaxic injection collagenase Ⅳ into caudate putamen,rats that underwent ICH were randomly divided into four groups:sham operation group,ICH group,PBS group,BMMNC-treated group.The BMMNCs were injected intravenously into rats after ICH.The neurobehavioral function was evaluated on days 1,3,7,14 by the modified neurological severity score,and the brain edema was examined by wet-dry weighting method on day 3 after cell transplantation.Immumofluorecence staining was used to identify the number of activation of microglia and infiltration of neutrophils in the brain after ICH.Results The neurological score in BMMNCtreated group on days 7,14 was significantly improved compared with those in ICH group and PBS group(P＜O.05).Compared to the ICH group ((81.09 ± 0.83) ％) and PBS group ((80.99 ± 0.79) ％),there was a significant decreasc in thc brain water content in BMMNC-treated group((78.62±0.97) ％) (P＜0.05).The number of activation of microglia and infiltration of neutrophils were both significantly lower in BMMNC-treated group ((55.8±22.1)/mm2,(49.6± 12.9)/mm2) compared to ICH group and PBS group (respectively (125.0 ± 20.7) /mm2,(86.8±13.6/mm2))(P＜0.01).Conclusion Administration of BMMNCs can significantly reduce edema and improve neurologic function by inhibiting the activation of microglia and infiltration of neutrophils.