CAJ | 학술논문

目的研究睡眠调节中枢下丘脑腹外侧视前区(VLPO)与觉醒调节关键区域结节乳头体核(TMN)之间的联系在大鼠睡眠-觉醒周期调控中的作用.方法将雄性SD大鼠随机分为对照组(TMN+ACSF&VLPO+ ACSF组)与实验组(TMN+ ACSF&VLPO+ L-Glu组,TMN+ Bic&VLPO+ ACSF组,TMN+Bic&VLPO+L-Glu组),采用脑立体定位技术确定大鼠VLPO以及TMN插管位置并进行核团内微量注射和多导睡眠描记技术等方法观察和记录大鼠睡眠的变化.结果与TMN+ACSF&VLPO+ACSF组(n=8)相比,TMN+ACSF&VLPO+L-Glu组(n=7)VLPO内微量注射L-谷氨酸(L-Glu)可使大鼠觉醒减少(42.30±3.75,P＜0.01),非快动眼睡眠(NREM)和总睡眠时间(TST)增多(61.97±3.41,P＜0.01；77.68±3.74,P＜0.01),快动眼睡眠(REM)无明显改变,差异无统计学意义；与TMN+ACSF&VLPO+L-Glu组(n=7)相比,TMN+Bic&VLPO+L-Glu组(n=8)中荷包牡丹碱(Bicuculline,Bic)和L-Glu相继分别微量注射于TMN和VLPO后,大鼠觉醒增加(79.22±3.93,P＜0.01),NREM(34.82±3.23,P＜0.01)和REM(5.97± 1.10,P＜0.05)以及TST(40.79±3.92,P＜0.01)减少,即TMN注射Bic可拮抗L-Glu在VLPO引起的促睡眠作用.结论 VLPO可通过GABAA受体抑制TMN神经元活动而产生促睡眠作用.
목적 연구수면조절중추하구뇌복외측시전구(VLPO)여각성조절관건구역결절유두체핵(TMN)지간적련계재대서수면-각성주기조공중적작용.방법 장웅성SD대서수궤분위대조조(TMN+ACSF&VLPO+ ACSF조)여실험조(TMN+ ACSF&VLPO+ L-Glu조,TMN+ Bic&VLPO+ ACSF조,TMN+Bic&VLPO+L-Glu조),채용뇌입체정위기술학정대서VLPO이급TMN삽관위치병진행핵단내미량주사화다도수면묘기기술등방법관찰화기록대서수면적변화.결과 여TMN+ACSF&VLPO+ACSF조(n=8)상비,TMN+ACSF&VLPO+L-Glu조(n=7)VLPO내미량주사L-곡안산(L-Glu)가사대서각성감소(42.30±3.75,P＜0.01),비쾌동안수면(NREM)화총수면시간(TST)증다(61.97±3.41,P＜0.01；77.68±3.74,P＜0.01),쾌동안수면(REM)무명현개변,차이무통계학의의；여TMN+ACSF&VLPO+L-Glu조(n=7)상비,TMN+Bic&VLPO+L-Glu조(n=8)중하포모단감(Bicuculline,Bic)화L-Glu상계분별미량주사우TMN화VLPO후,대서각성증가(79.22±3.93,P＜0.01),NREM(34.82±3.23,P＜0.01)화REM(5.97± 1.10,P＜0.05)이급TST(40.79±3.92,P＜0.01)감소,즉TMN주사Bic가길항L-Glu재VLPO인기적촉수면작용.결론 VLPO가통과GABAA수체억제TMN신경원활동이산생촉수면작용.
Objective To investigate the effects of relationship between ventrolateral preoptic nucleus (VLPO) and tuberomammillary nucleus (TMN) on the regulation of sleep-wake cycle in rats.Methods SD male rats were randomly divided into control group (TMN +ACSF&VLPO+ACSF) and experimental groups (TMN+ ACSF&VLPO+L-Glu,TMN+Bic & VLPO+ACSF,TMN+Bic&VLPO+L-Glu).Brain stereotaxic technique,microinjection and polysomnography were used to detect and record the variation of rat sleep.Results Compared with the TMN+ACSF&VLPO+ACSF group (n=8),the amount of wakefulness declined (42.30±3.75,P＜0.01) along with the enhancement of non-rapid eye movement (NREM) sleep (61.97±3.41,P＜0.01) and total sleep time (TST) (77.68±3.74,P＜0.01) after L-Glu microinjected into VLPO (n=7),while there was no significant change in rapid eye movement (REM) sleep.Compared to those of L-Glu microinjected into VLPO alone in the TMN +ACSF&VLPO+L-Glu group (n=7),the wakefulness increased (79.22±3.93,P＜0.01),meanwhile,NREM sleep (34.82±3.23,P＜0.01),REM sleep (5.97± 1.10,P＜0.05) and TST (40.79±3.92,P＜0.01) decrease after L-Glu microinjected into VLPO followed by the Bic microinjected into TMN (n=8).Conclusion VLPO can inhibit the neuronal activity of TMN via GABAA receptor and induce the sleep-promoting effect.