中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2014年
2期
110-113
,共4页
郭丽%郭艳峰%赵宗茂%马树成%孙国柱%刘晓鹏
郭麗%郭豔峰%趙宗茂%馬樹成%孫國柱%劉曉鵬
곽려%곽염봉%조종무%마수성%손국주%류효붕
脑缺血%胰岛素样生长因子-1%碱性成纤维生长因子
腦缺血%胰島素樣生長因子-1%堿性成纖維生長因子
뇌결혈%이도소양생장인자-1%감성성섬유생장인자
Ischemia%Insulin-like growth factor-1%Basic fibroblast growth factor
目的 探讨胰岛素样生长因子-Ⅰ(IGF-1)对脑缺血大鼠神经功能及碱性成纤维生长因子(bFGF)蛋白和bFGF mRNA表达的影响.方法 72只SD大鼠随机分为假手术组、脑缺血组和IGF-1治疗组,制备脑缺血模型,分别于脑缺血后12h、24 h、3d、7d采用改良mNSS评分量表评价大鼠的运动、感觉、平衡功能及反射等神经功能,采用免疫组织化学方法检测各组大鼠脑缺血皮层bFGF蛋白表达的变化,采用RT-PCR方法检测各组大鼠脑缺血皮层bFGF mRNA表达的变化.结果 在12h、24 h、3d、7d各时间点,IGF-1治疗组的mNSS评分值[(8.67±1.21)分,(7.50±1.52)分,(4.33±1.03)分,(3.67±1.37)分]均低于脑缺血组[(11.0±1.26)分,(9.83±1.33)分,(7.83±1.17)分,(7.17±1.72)分](P＜0.05).IGF-1治疗组各时间点bFGF蛋白表达均较脑缺血组升高(P＜0.05),尤其是脑缺血后12h有统计学意义(P＜0.01).IGF-1治疗组各时间点bFGF mRNA表达均较脑缺血组高(P＜0.05),脑缺血后24 h有统计学意义(P＜0.01).结论 IGF-1可促进脑缺血大鼠的神经功能恢复,可能与调控bFGF蛋白和bFGF mRNA表达有关.
目的 探討胰島素樣生長因子-Ⅰ(IGF-1)對腦缺血大鼠神經功能及堿性成纖維生長因子(bFGF)蛋白和bFGF mRNA錶達的影響.方法 72隻SD大鼠隨機分為假手術組、腦缺血組和IGF-1治療組,製備腦缺血模型,分彆于腦缺血後12h、24 h、3d、7d採用改良mNSS評分量錶評價大鼠的運動、感覺、平衡功能及反射等神經功能,採用免疫組織化學方法檢測各組大鼠腦缺血皮層bFGF蛋白錶達的變化,採用RT-PCR方法檢測各組大鼠腦缺血皮層bFGF mRNA錶達的變化.結果 在12h、24 h、3d、7d各時間點,IGF-1治療組的mNSS評分值[(8.67±1.21)分,(7.50±1.52)分,(4.33±1.03)分,(3.67±1.37)分]均低于腦缺血組[(11.0±1.26)分,(9.83±1.33)分,(7.83±1.17)分,(7.17±1.72)分](P＜0.05).IGF-1治療組各時間點bFGF蛋白錶達均較腦缺血組升高(P＜0.05),尤其是腦缺血後12h有統計學意義(P＜0.01).IGF-1治療組各時間點bFGF mRNA錶達均較腦缺血組高(P＜0.05),腦缺血後24 h有統計學意義(P＜0.01).結論 IGF-1可促進腦缺血大鼠的神經功能恢複,可能與調控bFGF蛋白和bFGF mRNA錶達有關.
목적 탐토이도소양생장인자-Ⅰ(IGF-1)대뇌결혈대서신경공능급감성성섬유생장인자(bFGF)단백화bFGF mRNA표체적영향.방법 72지SD대서수궤분위가수술조、뇌결혈조화IGF-1치료조,제비뇌결혈모형,분별우뇌결혈후12h、24 h、3d、7d채용개량mNSS평분량표평개대서적운동、감각、평형공능급반사등신경공능,채용면역조직화학방법검측각조대서뇌결혈피층bFGF단백표체적변화,채용RT-PCR방법검측각조대서뇌결혈피층bFGF mRNA표체적변화.결과 재12h、24 h、3d、7d각시간점,IGF-1치료조적mNSS평분치[(8.67±1.21)분,(7.50±1.52)분,(4.33±1.03)분,(3.67±1.37)분]균저우뇌결혈조[(11.0±1.26)분,(9.83±1.33)분,(7.83±1.17)분,(7.17±1.72)분](P＜0.05).IGF-1치료조각시간점bFGF단백표체균교뇌결혈조승고(P＜0.05),우기시뇌결혈후12h유통계학의의(P＜0.01).IGF-1치료조각시간점bFGF mRNA표체균교뇌결혈조고(P＜0.05),뇌결혈후24 h유통계학의의(P＜0.01).결론 IGF-1가촉진뇌결혈대서적신경공능회복,가능여조공bFGF단백화bFGF mRNA표체유관.
Objective To observe dynamically the influence of Insulin-like growth factor-1 (IGF-1) on the nerve function and expression of bFGF protein and Basic fibroblast growth factor(bFGF) mRNA after cerebral ischemic reperfusion in rats.Methods Seventy-two SD rats were randomly divided into sham-operated group,cerebral ischemia group,and IGF-1 treated group.The rat model of middle cerebral artery occlusion (MCAO) and reperfusion was performed.The evaluation of etiology was performed with mNSS at 12 h,24 h,3 d,7 d after ischemia-reperfusion,expression of bFGF protein was determined with immunohistochemical technique and expression of bFGF mRNA was determined with RT-PCR.Results The ratings of mNSS in IGF-1 treated group((8.67± 1.21),(7.50± 1.52),(4.33± 1.03),(3.67± 1.37)) were lower than those in ischemia group((11.0±1.26),(9.83±1.33),(7.83±1.17),(7.17±1.72) at 12 h,24 h,3 d or7 d after reperfusion(P＜0.05).For the IGF-1 treated group,the expression level of bFGF protein was higher than that of the cerebral ischemia group (P＜0.05),especially at 12 h after reperfusion (P＜0.01).The expression level of bFGF mRNA in the IGF-1 treated group was higher than that of the cerebral ischemia group (P＜ 0.05),especially at 24h after reperfusion (P＜ 0.01).Conclusion IGF-1 treatment has a protective effects on cerebral ischemia injury,which may contribute to its action on regulating expression of bFGF protein and bFGF mRNA.
