CAJ | 학술논문

目的观察中药雏菊叶龙胆(Bellidifolin)对坐骨神经损伤后的大鼠是否有促进神经功能恢复的作用,并探讨其机制中是否有睫状神经生长因子的参与.方法雄性Wistar大鼠225只,制备坐骨神经损伤模型,大鼠右侧坐骨神经离断,手术显微镜下缝合,取左侧为空白对照.造模成功后,大鼠随机分为对照组、雏菊叶龙胆25 mg组、50 mg组、75 mg组和甲钴胺组共5组,每组45只.对照组给予生理盐水腹腔注射,其余各组于手术后每天给予相应剂量的雏菊叶龙胆及甲钴胺腹腔注射,1,3及5周后进行足踪分析,计算坐骨神经功能指数(Sciatic Functional Index,SFl)、小腿三头肌湿重,免疫组化检测坐骨神经中组织睫状神经营养因子的变化(Ciliary Neurotrophic Factor,CNTF).结果 3周时SFI检测结果显示,对照组、甲钴胺组及雏菊叶龙胆25 mg、50 mg、75 mg组分别为:-84.35±4.87,-45.20±2.3,-70.42±4.2,-57.73±3.46和-64.38±4.38,其中对照组与各组相比差异有统计学意义(P＜0.05),甲钴胺组与雏菊叶龙胆各组相比结果差异有统计学意义(P＜0.05),雏菊叶龙胆组各组间相比,25 mg、75 mg与50 mg组相比差异有统计学意义(P＜0.05).小腿三头肌湿重恢复率比较,雏菊叶龙胆各组与对照组、甲钴胺组相比差异有统计学意义(P＜0.05),其中雏菊叶龙胆组中25 mg.75 mg组与50 mg组相比,差异有统计学意义(P＜0.05).3周时各组进行CNTF免疫组化比较,可见雏菊叶龙胆组25 mg、70 mg组与50 mg组相比差异有统计学意义(Jp＜0.05).结论雏菊叶龙胆可以促进损伤坐骨神经的功能恢复,50 mg作用最明显,神经组织内CNTF因子发挥重要作用.
목적 관찰중약추국협룡담(Bellidifolin)대좌골신경손상후적대서시부유촉진신경공능회복적작용,병탐토기궤제중시부유첩상신경생장인자적삼여.방법 웅성Wistar대서225지,제비좌골신경손상모형,대서우측좌골신경리단,수술현미경하봉합,취좌측위공백대조.조모성공후,대서수궤분위대조조、추국협룡담25 mg조、50 mg조、75 mg조화갑고알조공5조,매조45지.대조조급여생리염수복강주사,기여각조우수술후매천급여상응제량적추국협룡담급갑고알복강주사,1,3급5주후진행족종분석,계산좌골신경공능지수(Sciatic Functional Index,SFl)、소퇴삼두기습중,면역조화검측좌골신경중조직첩상신경영양인자적변화(Ciliary Neurotrophic Factor,CNTF).결과 3주시SFI검측결과현시,대조조、갑고알조급추국협룡담25 mg、50 mg、75 mg조분별위:-84.35±4.87,-45.20±2.3,-70.42±4.2,-57.73±3.46화-64.38±4.38,기중대조조여각조상비차이유통계학의의(P＜0.05),갑고알조여추국협룡담각조상비결과차이유통계학의의(P＜0.05),추국협룡담조각조간상비,25 mg、75 mg여50 mg조상비차이유통계학의의(P＜0.05).소퇴삼두기습중회복솔비교,추국협룡담각조여대조조、갑고알조상비차이유통계학의의(P＜0.05),기중추국협룡담조중25 mg.75 mg조여50 mg조상비,차이유통계학의의(P＜0.05).3주시각조진행CNTF면역조화비교,가견추국협룡담조25 mg、70 mg조여50 mg조상비차이유통계학의의(Jp＜0.05).결론 추국협룡담가이촉진손상좌골신경적공능회복,50 mg작용최명현,신경조직내CNTF인자발휘중요작용.
Objective To study whether bellidifolin has rehabilitation effect on injured sciatic nerve rats,and whether ciliary neurotrophic factor (CNTF) is involved in this mechanism.Methods 225 male wistar rats were made to be sciatic nerve injured models,with right sciatic nerve being cut and sewed under microscopy,left sciatic being sham.Rats were randomly divided into control group,bellidifolin 25 mg,50 mg,75 mg groups and mecobalamin group,with 45 rats in each group.Rats in control group were just injected sodium chloride,others were intra-peritonealiy injected different doses of bellidifolin and mecobalamin after operation.Results Three weeks after operation,SFl of control group,mecobalaming group,bellidifolin 25 mg,50 mg and 75 mg group were-84.35± 4.87,-45.20±2.30,-70.42±4.21,-57.73±3.46 and-64.38±4.38 respectively.Compared with control group,others showed significant differences (P＜0.05).There were statistically differences between bellidifolin groups and mecobalaming group(P＜0.05).Within bellidifolin groups,50 mg group showed difference compared with 25 mg and 75 rg groups(P=0.031).TSW results also showed differences among bellidifolin groups,control group and mecobalaming group.There were statistical differences among bellidifolin groups(P＜0.05).Each groups with immunohistochemistry analysis,CNTF expression showed statistically differences among bellidifolin 50 mg group and 25 mg,70 rg groups,Bellidifolin 50 mg group was higher than others(P＜0.05).Conclusion Bellidifolin can promote the recovery of injured sciatic nerve,especially the concentration of 50 mg bellidifolin,and CNTF is involved in the rehablitation process.