CAJ | 학술논문

目的研究皮质酮与应激在大鼠的创伤后应激障碍(PTSD)样记忆损害中的作用及交互效应.方法分别以0 mA、0.8 mA及1.4 mA的电击量对大鼠进行预实验.首先大鼠接受恐惧任务训练,训练后24 h进行场景恐惧记忆测试,然后采用泛化测试验证大鼠PTSD样记忆损害模型是否复制成功.为了观察糖皮质激素的作用,采用双侧肾上腺切除术,并以皮质酮替代饲养,大鼠在手术后7天进行行为学实验.根据是否接受皮质酮替代及电击量分为四组:空白对照组(无皮质酮替代,电击量0 mA)、皮质酮组(皮质酮替代,电击量0 mA)、电击组(无皮质酮替代,电击量1.4 mA)、皮质酮-电击组(皮质酮替代,电击量1.4 mA).采用析因设计资料的方差分析,对皮质酮与电击的交互作用进行统计分析.结果 0.8 mA组与0 mA组比较,大鼠的僵住反应百分比升高;0.8 mA组与1.4 mA组比较,大鼠的僵住反应百分比下降,并且1.4 mA组出现泛化效应.在析因设计资料的方差分析中,皮质酮-电击组与其他各组的僵住反应百分比均差异有统计学意义(P＜0.05),其他各组之间差异无统计学意义.皮质酮(F=6.464,P＜0.05)和电击(F=53.419,P＜0.01)均对PTSD样记忆损害形成发挥作用,且皮质酮与电击之间存在交互效应(F=11.580,P＜0.05).结论高电击量(1.4 mA)能够诱导出大鼠PTSD样记忆损害,皮质酮与应激在此过程中存在交互效应.
목적 연구피질동여응격재대서적창상후응격장애(PTSD)양기억손해중적작용급교호효응.방법 분별이0 mA、0.8 mA급1.4 mA적전격량대대서진행예실험.수선대서접수공구임무훈련,훈련후24 h진행장경공구기억측시,연후채용범화측시험증대서PTSD양기억손해모형시부복제성공.위료관찰당피질격소적작용,채용쌍측신상선절제술,병이피질동체대사양,대서재수술후7천진행행위학실험.근거시부접수피질동체대급전격량분위사조:공백대조조(무피질동체대,전격량0 mA)、피질동조(피질동체대,전격량0 mA)、전격조(무피질동체대,전격량1.4 mA)、피질동-전격조(피질동체대,전격량1.4 mA).채용석인설계자료적방차분석,대피질동여전격적교호작용진행통계분석.결과 0.8 mA조여0 mA조비교,대서적강주반응백분비승고;0.8 mA조여1.4 mA조비교,대서적강주반응백분비하강,병차1.4 mA조출현범화효응.재석인설계자료적방차분석중,피질동-전격조여기타각조적강주반응백분비균차이유통계학의의(P＜0.05),기타각조지간차이무통계학의의.피질동(F=6.464,P＜0.05)화전격(F=53.419,P＜0.01)균대PTSD양기억손해형성발휘작용,차피질동여전격지간존재교호효응(F=11.580,P＜0.05).결론 고전격량(1.4 mA)능구유도출대서PTSD양기억손해,피질동여응격재차과정중존재교호효응.
Objective To investigate the interactional roles corticosterone and stress played during the PTSD-Like memory impairment.Methods First we established the model of PTSD-like memory using three levels of electric shock:0 mA,0.8 mA and 1.4 mA.The freezing time percent of each group were calculated 24 hours later in order to find which group of rats obtained the PTSD-like memory,and generalizing tests was used to verify it.After the model was established,rats received adrenalectomy (ADX) were operated bilaterally in order to investigate the effect of corticosterone and fed with corticosterone in the drinking water 7 days before fear conditioning.These rats were divided into four groups:control group (no corticosterone supplement,0 mA shock),Cort group (corticosterone supplement,0 mA shock),shock group (no corticosterone supplement,1.4 mA shock),Cort-shock group (corticosterone supplement,1.4 mA shock).A two-way factorial analysis of variance was used to determine whether there was a significant interaction between corticosterone and shock.Results The freezing time percent of group 0.8 mA raised compared with the group 0 mA,whereas the freezing time percent of group 1.4 mA showed reversely and the generalizing effects appeared,compared with group 0.8 mA.In two-way factorial analysis design,the freezing time percent of Cort-shock group significantly increased (P＜0.05) compared with other groups,and there was no significant variance among the other groups.Corticosterone (F=6.464,P＜0.05) and stress (F=53.419,P ＜0.01) played parts in the formation of PTSD-like memory impairment,and they interacted with each other (F=11.580,P＜0.05).Conclusion PTSD-like memory impairment can be induced on rats with high (1.4 mA) electrical shock,and they have interactional effect with each other in the process.