中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2014年
5期
385-387
,共3页
杨许丽%刘玥%侯百灵%刘明%夏天娇%孙蓓%张羽%冷鑫%石林玉
楊許麗%劉玥%侯百靈%劉明%夏天嬌%孫蓓%張羽%冷鑫%石林玉
양허려%류모%후백령%류명%하천교%손배%장우%랭흠%석림옥
骨肿瘤%疼痛%CREB转录共激活因子1%脊髓
骨腫瘤%疼痛%CREB轉錄共激活因子1%脊髓
골종류%동통%CREB전록공격활인자1%척수
Bone neoplasms%Pain%CREB-regulated transcription coactivator 1%Spinal cord
目的 探讨骨癌痛小鼠脊髓水平CREB转录共激活因子1(CRTC1)表达的变化.方法 采用随机数字表法,46只C3H/HeJ雄性小鼠随机分为假手术组(Sham组,n=23)和肿瘤组(Tumor组,n=23),Tumor组小鼠在右侧股骨远端骨髓腔接种溶于α-MEM的NCTC2472纤维肉瘤细胞,建立骨癌痛模型,Sham组小鼠接种不含肿瘤细胞的α-MEM.两组在术前1d、术后4d、7d、10 d、14 d、21 d检测痛行为学,并于相应时间点痛行为学检测后,分别随机选取3只小鼠处死,取脊髓L3-L5节段,采用Western Blot法检测CRTC1的蛋白表达水平.结果 与Sham组比较,Tumor组接种后各时间点机械缩足阈值[(1.35±0.14)g,(1.10±0.28)g,(0.78±0.25)g,(0.47±0.16)g,(0.34±0.16)g]明显降低(P＜0.05),自发抬足次数[(3.12±0.74)次,(6.02±0.67)次,(7.42±1.22)次,(10.82±0.98)次,(12.48±1.06)次]明显增加(P＜0.05).与基础值相比,Sham组和Tumor组脊髓水平CRTC1的表达在术后4d升高(P＜0.05);与基础值和Sham组相比,Tumor组脊髓水平CRTC1的表达在术后7d、10d、14d、21 d均升高(P＜0.05).结论 骨癌痛小鼠脊髓水平CRTC1的蛋白表达上调,该变化可能参与了骨癌痛的发生和维持.
目的 探討骨癌痛小鼠脊髓水平CREB轉錄共激活因子1(CRTC1)錶達的變化.方法 採用隨機數字錶法,46隻C3H/HeJ雄性小鼠隨機分為假手術組(Sham組,n=23)和腫瘤組(Tumor組,n=23),Tumor組小鼠在右側股骨遠耑骨髓腔接種溶于α-MEM的NCTC2472纖維肉瘤細胞,建立骨癌痛模型,Sham組小鼠接種不含腫瘤細胞的α-MEM.兩組在術前1d、術後4d、7d、10 d、14 d、21 d檢測痛行為學,併于相應時間點痛行為學檢測後,分彆隨機選取3隻小鼠處死,取脊髓L3-L5節段,採用Western Blot法檢測CRTC1的蛋白錶達水平.結果 與Sham組比較,Tumor組接種後各時間點機械縮足閾值[(1.35±0.14)g,(1.10±0.28)g,(0.78±0.25)g,(0.47±0.16)g,(0.34±0.16)g]明顯降低(P＜0.05),自髮抬足次數[(3.12±0.74)次,(6.02±0.67)次,(7.42±1.22)次,(10.82±0.98)次,(12.48±1.06)次]明顯增加(P＜0.05).與基礎值相比,Sham組和Tumor組脊髓水平CRTC1的錶達在術後4d升高(P＜0.05);與基礎值和Sham組相比,Tumor組脊髓水平CRTC1的錶達在術後7d、10d、14d、21 d均升高(P＜0.05).結論 骨癌痛小鼠脊髓水平CRTC1的蛋白錶達上調,該變化可能參與瞭骨癌痛的髮生和維持.
목적 탐토골암통소서척수수평CREB전록공격활인자1(CRTC1)표체적변화.방법 채용수궤수자표법,46지C3H/HeJ웅성소서수궤분위가수술조(Sham조,n=23)화종류조(Tumor조,n=23),Tumor조소서재우측고골원단골수강접충용우α-MEM적NCTC2472섬유육류세포,건립골암통모형,Sham조소서접충불함종류세포적α-MEM.량조재술전1d、술후4d、7d、10 d、14 d、21 d검측통행위학,병우상응시간점통행위학검측후,분별수궤선취3지소서처사,취척수L3-L5절단,채용Western Blot법검측CRTC1적단백표체수평.결과 여Sham조비교,Tumor조접충후각시간점궤계축족역치[(1.35±0.14)g,(1.10±0.28)g,(0.78±0.25)g,(0.47±0.16)g,(0.34±0.16)g]명현강저(P＜0.05),자발태족차수[(3.12±0.74)차,(6.02±0.67)차,(7.42±1.22)차,(10.82±0.98)차,(12.48±1.06)차]명현증가(P＜0.05).여기출치상비,Sham조화Tumor조척수수평CRTC1적표체재술후4d승고(P＜0.05);여기출치화Sham조상비,Tumor조척수수평CRTC1적표체재술후7d、10d、14d、21 d균승고(P＜0.05).결론 골암통소서척수수평CRTC1적단백표체상조,해변화가능삼여료골암통적발생화유지.
Objective To investigate the changes in the expression of CREB-regulated transcription coactivator 1 (CRTC1) in spinal cord during the development and maintenance of bone cancer pain in mice.Methods Forty six male C3H/HeJ mice were randomly divided into Sham group (n=23) and Tumor group (n=23).The mouse model of bone cancer pain was developed by intra-femur inoculation of α-minimal essence media (α-MEM) with osteolytic NCTC 2472 cells.The mice in sham group were inoculated with α-MEM without NCTC 2472 cells.Pain behaviors such as the paw withdrawal mechanical threshold (PWMT) and the number of spontaneous flinches (NSF) were assessed on 1 d before inoculation and on 4 d,7 d,10 d,14 d,21 d after inoculation.At each corresponding time point,three mice of each group were killed just after the pain behaviors assessment and the samples of spinal cord lumbar segment were obtained to detect the expression of CRTC1 using Western Blot.Results Compared with Sham group,PWMT((1.35±0.14) g,(1.10±0.28) g,(0.78±0.25) g,(0.47±0.16) g,(0.34±0.16)g) was significantly decreased (P＜0.05) and NSF((3.12±0.74),(6.02±0.67),(7.42± 1.22),(10.824±0.98),(12.48±1.06)) was significantly increased (P＜0.05) on 7 d,10 d,14 d and 21 d aftcr inoculation in Tumor group.Compared with baseline level,significant increase in the expression of CRTC1 in spinal cord was observed on 4 d in both Sham group and Tumor group (P＜0.05).Compared with baseline level and Sham group,significant increase in the expression of CRTC1 in spinal cord was observed on 7 d,10 d,14 d and 21 d in Tumor group (P＜0.05).Conclusion Expression of CRTC1 in spinal cord is up-regulated in mice with bone cancer pain,and this change may be involved in the development and maintenance of bone cancer pain.
