中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2014年
5期
394-397
,共4页
母爱剥夺%抑郁行为%儿茶酚胺
母愛剝奪%抑鬱行為%兒茶酚胺
모애박탈%억욱행위%인다분알
Maternal deprivation%Depression behavior%Catecholamine
目的 探讨不同时段哺乳期母爱剥夺对子代小鼠成年后抑郁情绪的影响.方法 新生SD大鼠随机分为5个不同时段母爱剥夺组(n=5×5)和对照组(n=7).母爱剥夺组在不同的年龄阶段进行连续5d母爱剥夺(出生当天为p0,出生后第5天为p5,出生后第10天为p10,出生后第15天为p15,出生后第20天为p20),剥夺期间每天进行6h的母婴分离.当这些大鼠长到10周通过强迫性游泳训练、毛发梳理、葡萄糖消耗实验来评定抑郁行为,同时通过高效液相色谱法测定大脑中儿茶酚胺类神经调质的改变.结果 母爱剥夺组大鼠表现出明显的主动游泳降低,静止漂浮时间从对照组的(58.5±7.5)s,增加到p0:(75.6±9.4) s,p5:(150.4±12.5) s,p10:(174.5±14.3) s,p15:(155.4±11.4) s,p20:(112.4±10.3)s(均P＜0,01,n=5～7).与对照组(0.87±0.1)比较,母爱剥夺组糖水消耗降低[p0(0.73±0.12),p5(0.53±0.09),p10(0.5±0.06),p15(0.54±0.07),p20(0.67±0.05)].毛发梳理得分从对照组的(4.5±0.6)分,降低到p0:(3.3±0.6)分,p5:(2.3±0.4)分,p10:(1.9±0.3)分,p15:(2.4±0.4)分,p20:(4.4±0.4)分.与对照组相比,母爱剥夺组大鼠脑内儿茶酚胺水平降低(分别是p0:(94.5±11.5)％;p5:(78.5±9.6)％;p10:(67.6±7.4)％;p15:(64.5±6.6)％;p20:(88.5±7.5)％,均P＜0.01,n=5～7).结论 出生后哺乳期内10 d左右的母爱剥夺可以明显引起抑郁行为,导致大脑内儿茶酚胺类神经调质的改变.
目的 探討不同時段哺乳期母愛剝奪對子代小鼠成年後抑鬱情緒的影響.方法 新生SD大鼠隨機分為5箇不同時段母愛剝奪組(n=5×5)和對照組(n=7).母愛剝奪組在不同的年齡階段進行連續5d母愛剝奪(齣生噹天為p0,齣生後第5天為p5,齣生後第10天為p10,齣生後第15天為p15,齣生後第20天為p20),剝奪期間每天進行6h的母嬰分離.噹這些大鼠長到10週通過彊迫性遊泳訓練、毛髮梳理、葡萄糖消耗實驗來評定抑鬱行為,同時通過高效液相色譜法測定大腦中兒茶酚胺類神經調質的改變.結果 母愛剝奪組大鼠錶現齣明顯的主動遊泳降低,靜止漂浮時間從對照組的(58.5±7.5)s,增加到p0:(75.6±9.4) s,p5:(150.4±12.5) s,p10:(174.5±14.3) s,p15:(155.4±11.4) s,p20:(112.4±10.3)s(均P＜0,01,n=5～7).與對照組(0.87±0.1)比較,母愛剝奪組糖水消耗降低[p0(0.73±0.12),p5(0.53±0.09),p10(0.5±0.06),p15(0.54±0.07),p20(0.67±0.05)].毛髮梳理得分從對照組的(4.5±0.6)分,降低到p0:(3.3±0.6)分,p5:(2.3±0.4)分,p10:(1.9±0.3)分,p15:(2.4±0.4)分,p20:(4.4±0.4)分.與對照組相比,母愛剝奪組大鼠腦內兒茶酚胺水平降低(分彆是p0:(94.5±11.5)％;p5:(78.5±9.6)％;p10:(67.6±7.4)％;p15:(64.5±6.6)％;p20:(88.5±7.5)％,均P＜0.01,n=5～7).結論 齣生後哺乳期內10 d左右的母愛剝奪可以明顯引起抑鬱行為,導緻大腦內兒茶酚胺類神經調質的改變.
목적 탐토불동시단포유기모애박탈대자대소서성년후억욱정서적영향.방법 신생SD대서수궤분위5개불동시단모애박탈조(n=5×5)화대조조(n=7).모애박탈조재불동적년령계단진행련속5d모애박탈(출생당천위p0,출생후제5천위p5,출생후제10천위p10,출생후제15천위p15,출생후제20천위p20),박탈기간매천진행6h적모영분리.당저사대서장도10주통과강박성유영훈련、모발소리、포도당소모실험래평정억욱행위,동시통과고효액상색보법측정대뇌중인다분알류신경조질적개변.결과 모애박탈조대서표현출명현적주동유영강저,정지표부시간종대조조적(58.5±7.5)s,증가도p0:(75.6±9.4) s,p5:(150.4±12.5) s,p10:(174.5±14.3) s,p15:(155.4±11.4) s,p20:(112.4±10.3)s(균P＜0,01,n=5～7).여대조조(0.87±0.1)비교,모애박탈조당수소모강저[p0(0.73±0.12),p5(0.53±0.09),p10(0.5±0.06),p15(0.54±0.07),p20(0.67±0.05)].모발소리득분종대조조적(4.5±0.6)분,강저도p0:(3.3±0.6)분,p5:(2.3±0.4)분,p10:(1.9±0.3)분,p15:(2.4±0.4)분,p20:(4.4±0.4)분.여대조조상비,모애박탈조대서뇌내인다분알수평강저(분별시p0:(94.5±11.5)％;p5:(78.5±9.6)％;p10:(67.6±7.4)％;p15:(64.5±6.6)％;p20:(88.5±7.5)％,균P＜0.01,n=5～7).결론 출생후포유기내10 d좌우적모애박탈가이명현인기억욱행위,도치대뇌내인다분알류신경조질적개변.
Objective To investigate the effects of maternal deprivation at different time periods in rat pup development on depressions in adults.Methods Newborn SD rat pups were randomly divided into 5 maternal deprivation groups(n=5 in each group) and one control group (n=7).The rat pups in the maternal deprivation groups were separated from their mother for 6 hours in one day,at p0,p5,p 10,p15,p20 respectively,lasting for five days.Then the rats will be screened for depression at the age of 10 weeks,using different behavioral tests,which included forced swim,coat score,sucrose preference test.Norepinephrine concentration in the cerebral spinal fluid was also measured through high performance liquid chromatography (HPLC).Results Maternal deprivation for rat pups induced depression like behavior in adults.The floating time during forced swim test increased from(58.5 ±7.5) s in control group to(p0(75.6±9.4) s,p5(150.4±12.5) s,p10(174.5±14.3) s,p15(155.4±11.4)s,p20 (112.4±10.3) s respectively in maternal deprivation groups;and it was significant different among the groups(P＜ 0.01).Compared with the control group(0.87±0.1),the sucrose preference in maternal deprivation groups decreased(p0 (0.73±0.12),p5 (0.53±0.09),p10 (0.5±0.06),p15 (0.54±0.07),p20 (0.67±0.05)).And the coating scores in maternal deprivation groups decreased from 4.5±0.6 in control group to p0(3.3±0.6),p5 (2.3±0.4),p10 (1.9±0.3),p15 (2.4±0.4),p20 (4.4± 0.4).Finally,maternal deprivation also induced low concentration of catecholamine in the cerebral spinal fluid compared with that in control group (p0 (94.5± 11.5) ％,p5 (78.5± 9.6) ％,p10(67.6±±7.4)％,p1S(64.5±6.6)％,p20(88.5±7.5)％,P＜0.01,n=5-7).Conclusion Maternal deprivation at different lactation periods may induce depression behaviors in rat offspring,and also lead to lower concentration of catecholamine in the cerebral spinal fluid.
