中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2014年
6期
524-527
,共4页
王宾%巩万坤%王峰%黄宏平%汪萌芽
王賓%鞏萬坤%王峰%黃宏平%汪萌芽
왕빈%공만곤%왕봉%황굉평%왕맹아
慢性不可预见性应激%大鼠%蓝斑%下丘脑%育亨宾
慢性不可預見性應激%大鼠%藍斑%下丘腦%育亨賓
만성불가예견성응격%대서%람반%하구뇌%육형빈
Chronic unpredictable stress%Rats%Locus coeruleus%Hypothalamus%Yohimbine
目的 在体电刺激蓝斑核,探讨抑郁样行为模型大鼠下丘脑内的去甲肾上腺素(NE)释放调控机制.方法 应用慢性不可预见性应激刺激结合孤养建立抑郁样行为大鼠模型,通过行为学检测观察造模是否成功.在体电刺激两组大鼠蓝斑核,应用碳纤维电极测量技术记录下丘脑内NE的释放量,分析两组大鼠NE释放信号的峰值、达峰时间和半衰期三个指标.结果 行为学检测表明模型组大鼠的体质量[(263.9±16.4)g]、旷场实验得分[(19.4±7.9)分]和糖水偏爱百分比[(44.3±10.8)％]低于对照组[(314.3±24.3)g,(53.3±19.0)分,(60.6±13.3)％](P＜0.01).电刺激蓝斑核,在下丘脑通过微碳纤电极检测NE释放,模型组大鼠NE释放的峰值[(176.9±50.4) pA]比对照组大鼠减少[(361.6±88.6) pA] (P＜0.01),达峰时间缩短(P＜0.05);在腹腔注射α2受体拮抗剂育亨宾(3 mg/kg)后,模型组大鼠NE释放的峰值和达峰时间均明显增大,差异有统计学意义.对照组大鼠用育亨宾(3 mg/kg)后NE信号的峰值和达峰时间虽然有所增加,但差异无统计学意义.结论 慢性不可预见性应激导致的抑郁样行为模型大鼠的蓝斑核投射到下丘脑的神经末梢释放的NE减少;育亨宾可以通过抑制突触前α2受体增加模型组大鼠蓝斑核投射至下丘脑NE的释放.结果提示蓝斑投射到下丘脑的去甲肾上腺素能通路可能参与到抑郁症的发生.
目的 在體電刺激藍斑覈,探討抑鬱樣行為模型大鼠下丘腦內的去甲腎上腺素(NE)釋放調控機製.方法 應用慢性不可預見性應激刺激結閤孤養建立抑鬱樣行為大鼠模型,通過行為學檢測觀察造模是否成功.在體電刺激兩組大鼠藍斑覈,應用碳纖維電極測量技術記錄下丘腦內NE的釋放量,分析兩組大鼠NE釋放信號的峰值、達峰時間和半衰期三箇指標.結果 行為學檢測錶明模型組大鼠的體質量[(263.9±16.4)g]、曠場實驗得分[(19.4±7.9)分]和糖水偏愛百分比[(44.3±10.8)％]低于對照組[(314.3±24.3)g,(53.3±19.0)分,(60.6±13.3)％](P＜0.01).電刺激藍斑覈,在下丘腦通過微碳纖電極檢測NE釋放,模型組大鼠NE釋放的峰值[(176.9±50.4) pA]比對照組大鼠減少[(361.6±88.6) pA] (P＜0.01),達峰時間縮短(P＜0.05);在腹腔註射α2受體拮抗劑育亨賓(3 mg/kg)後,模型組大鼠NE釋放的峰值和達峰時間均明顯增大,差異有統計學意義.對照組大鼠用育亨賓(3 mg/kg)後NE信號的峰值和達峰時間雖然有所增加,但差異無統計學意義.結論 慢性不可預見性應激導緻的抑鬱樣行為模型大鼠的藍斑覈投射到下丘腦的神經末梢釋放的NE減少;育亨賓可以通過抑製突觸前α2受體增加模型組大鼠藍斑覈投射至下丘腦NE的釋放.結果提示藍斑投射到下丘腦的去甲腎上腺素能通路可能參與到抑鬱癥的髮生.
목적 재체전자격람반핵,탐토억욱양행위모형대서하구뇌내적거갑신상선소(NE)석방조공궤제.방법 응용만성불가예견성응격자격결합고양건립억욱양행위대서모형,통과행위학검측관찰조모시부성공.재체전자격량조대서람반핵,응용탄섬유전겁측량기술기록하구뇌내NE적석방량,분석량조대서NE석방신호적봉치、체봉시간화반쇠기삼개지표.결과 행위학검측표명모형조대서적체질량[(263.9±16.4)g]、광장실험득분[(19.4±7.9)분]화당수편애백분비[(44.3±10.8)％]저우대조조[(314.3±24.3)g,(53.3±19.0)분,(60.6±13.3)％](P＜0.01).전자격람반핵,재하구뇌통과미탄섬전겁검측NE석방,모형조대서NE석방적봉치[(176.9±50.4) pA]비대조조대서감소[(361.6±88.6) pA] (P＜0.01),체봉시간축단(P＜0.05);재복강주사α2수체길항제육형빈(3 mg/kg)후,모형조대서NE석방적봉치화체봉시간균명현증대,차이유통계학의의.대조조대서용육형빈(3 mg/kg)후NE신호적봉치화체봉시간수연유소증가,단차이무통계학의의.결론 만성불가예견성응격도치적억욱양행위모형대서적람반핵투사도하구뇌적신경말소석방적NE감소;육형빈가이통과억제돌촉전α2수체증가모형조대서람반핵투사지하구뇌NE적석방.결과제시람반투사도하구뇌적거갑신상선소능통로가능삼여도억욱증적발생.
Objective To study the cellular and molecular modulation mechanisms of norepinephrine (NE) release in hypothalamus induced by electrical stimulation in locus coeruleus (LC) in the rat model of depression-like behavior.Methods The depression-like behavior model was established by combining separation and chronic unpredictable stress stimulations.After the model establishment,behavior tests were used to verify the success of modeling.NE release in the hypothalamus induced by electrical stimulation in LC was studied in real time and NE signal was recorded with carbon fiber electrode.The peak value,the time to peak and half-life period of NE signal in both group rats were measured and analysed.Results The bodyweight,score of open-field test,percentage of sucrose preference of model group rats ((263.9± 16.4) g,(19.4±7.9),(44.3± 10.8) ％) were significantly lower than those((314.3±24.3) g,(53.3± 19.0),(60.6± 13.3) ％) of control group(P＜0.01).The peak value of NE signal in the hypothalamus induced by electrical stimulation in locus coeruleus in depression-like behavior model rats((176.9±50.4) pA)was less than that((361.6±88.6) pA)in control group(P＜0.01),and the time to peak of NE signal was also shortened in depression-like behavior model group rats (P＜0.05).Intraperitoneal injection of yohimbine (3 mg/kg) potentiated electrical stimulation induced NE release in depression-like behavior model rats but not in control group.Conclusion The chronic unpredictable stresses attenuate the secretion of NE in the hypothalamus induced by electrical stimulation in LC in rats.Yohimbine,a presynaptic α2 receptor antagonist,increased NE release in hypothalamus in depression-like behavior model rats.These findings suggest that the LC projects functionally to the hypothalamus and the projection may contribute to the pathogenesis of depression.
